9,012 results

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c-myc promoter (Del 4)

Plasmid

#16604

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (Del 4) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Del-4 (PvuII->PvuII). The first PvuII site is …

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only

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pLVCT-rtTR-KRAB-2SM2

Plasmid

#11779

Purpose

Tet-regulated (Tet-off) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

CAG promoters, GFP, rtTR-KRAB-2SM2, Tet-off

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk2a1

Plasmid

#13754

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2a1 (MAPK9 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pGEX5X.1 PINK1 WT

Plasmid

#13321

Depositor

Mark Cookson

Article

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 kinase domain (PINK1 Human)

Tags

GST

Expression

Bacterial

Mutation

Amino acids 112-496

Available Since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only

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pBABE puro SDF-1 alpha

Plasmid

#12270

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

SDF-1 alpha (CXCL12 Human)

Use

Retroviral

Expression

Mammalian

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mCherry-VPS4A

Plasmid

#115334

Purpose

expresses mCherry-VPS4A in mammalian cells

Depositor

Sanford Simon

Article

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

VPS4A (VPS4A Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

silent mutation in position 4050 C to T

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pRK7-FLAG-TSC1

Plasmid

#8995

Depositor

John Blenis

Article

Tee et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

Insert

TSC1 (TSC1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 11, 2005

Availability

Academic Institutions and Nonprofits only

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N-Myc-53BP1 WT pLPC-Puro

Plasmid

#19836

Depositor

Titia de Lange

Article

Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

Insert

p53 Binding Protein 1 (TP53BP1 Human)

Use

Retroviral

Tags

Myc

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-PCP-GFPnls

Plasmid

#121938

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

PCP-GFPnls

Use

Lentiviral

Tags

sfGFP

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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STAT3(Y705F)-TAL-Luc

Plasmid

#46933

Purpose

Luciferase Reporter for mutant STAT3

Depositor

Afshin Dowlati

Article

Dabir et al Mol Cancer Res. 2009 Nov;7(11):1854-60.

Insert

STAT3(Y705F) (STAT3 Human)

Use

Luciferase

Tags

FLAG

Mutation

Tyrosine 705 to Phenylalanine

Promoter

TA Luciferase

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only
pLVX-EF1alpha-SARS-CoV-2-orf10-2xStrep-IRES-Puro

Plasmid

#141394

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf10 (ORF10 SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Adeno Cas9

Plasmid

#64072

Purpose

Adenoviral vector for the expression of the Cas9

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Type

Empty backbone

Use

Adenoviral

Tags

FLAG

Promoter

U6 and CBh

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pmCherry-ATG5-K130R

Plasmid

#13096

Depositor

Roberta Gottlieb

Article

Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

Insert

ATG5-K130R (Atg5 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

K130R

Available Since

Nov. 15, 2006

Availability

Academic Institutions and Nonprofits only

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CX-nestinGFPas

Plasmid

#32401

Depositor

Patrick Salmon, Henryk Zulewski

Article

Eberhardt et al Biochem Biophys Res Commun. 2006 Jul 7;3

Insert

nestin promoter/enhancer GFP

Use

Lentiviral

Available Since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

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pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42337

Purpose

Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pMB80 (R26-CreER)

Plasmid

#12168

Depositor

Tyler Jacks

Article

McLaughlin et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

Rosa26 CreER^T2

Use

Cre/Lox

Expression

Mammalian

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Cdc42-T17N

Plasmid

#12973

Depositor

Gary Bokoch

Insert

Cdc42 DN (CDC42 Human)

Tags

Myc

Expression

Mammalian

Mutation

T17N Dominant negative

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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p52 cFlag pcDNA3

Plasmid

#20019

Depositor

Stephen Smale

Insert

Nfkb2 (Nfkb2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Truncated at the p100 ligation site. It codes for…

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pZS157 CRISPEY RT/Cas9

Plasmid

#114454

Purpose

Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expression

Depositor

Hunter Fraser

Article

Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

Insert

SpCas9, Ec86-RT

Use

CRISPR

Tags

SV40-NLS

Expression

Yeast

Mutation

S. cerevisiae codon optimized

Promoter

GAL1-GAL10

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ350 - MCS(ttTi5605, II)

Plasmid

#34866

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

ttTi5605 targeting

Use

Worm targeting

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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