We narrowed to 7,669 results for: CCH

1. pIRHDGAL3C

   Plasmid

   #218276

   Purpose

   Introduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
   

   Expression

   Yeast

   Mutation

   GAL3 (S509P), DHFR (P66L)

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGG-GST-TEV-mCherry-NAP1 (WT)

   Plasmid

   #198036

   Purpose

   Used for the expression and purification of mCherry NAP1 in a mammalian expression system (SMC Internal No. 2065)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   NAP1 (AZI2 Human)
   

   Tags

   GST-TEV and mCherry

   Expression

   Mammalian

   Available Since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. YIp128-CUP1-His6-CUbo(K48R/K63R)-GFP

   Plasmid

   #212795

   Purpose

   Copper-inducible expression of the artificial test substrate His6-CUbo(K48R/K63R)-GFP in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   His6-CUbo(K48R/K63R)-GFP
   

   Use

   Integrative vector

   Expression

   Yeast

   Mutation

   CUb(K48R/K63R)

   Promoter

   CUP1

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas-Cas12m-ΔZF

   Plasmid

   #192276

   Purpose

   Encodes MmCas12m ΔZF (H549A, C552A) under a constitutive promoter

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   Cas12m ΔZF
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   H459A, C552A

   Promoter

   PJ23108

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGG-GST-TEV-NAP1 (WT)

   Plasmid

   #198034

   Purpose

   Used for the expression and purification of NAP1 in a mammalian expression system (SMC Internal No. 2064)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   NAP1 (AZI2 Human)
   

   Tags

   GST-TEV

   Expression

   Mammalian

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)

   Plasmid

   #198072

   Purpose

   Used for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Tags

   GST-TEV-EGFP

   Expression

   Insect

   Mutation

   TBK1ΔCTD (res 1-666)

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pROS_phleo-Tps1/Gsy2

   Plasmid

   #196612

   Purpose

   Encoding guide RNAs for the knock out of TPS1 and GSY2 genes

   Depositor

   Matthias Heinemann

   Article

   Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

   Insert

   TPS1, GSY2 (TPS1, GSY2 Budding Yeast)
   

   Expression

   Yeast

   Promoter

   SNR52

   Available Since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pROS13-Tps2/Gsy1

   Plasmid

   #196613

   Purpose

   Encoding guide RNAs for the knock out of TPS2 and GSY1 genes

   Depositor

   Matthias Heinemann

   Article

   Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

   Insert

   S000001911 (TPS2, GSY1 Budding Yeast)
   

   Expression

   Yeast

   Promoter

   SNR52

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUGP1.1

   Plasmid

   #196615

   Purpose

   Plasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeast

   Depositor

   Matthias Heinemann

   Article

   Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

   Insert

   UGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)
   

   Tags

   AID(71-114) and mCherry

   Expression

   Yeast

   Promoter

   Native promoter

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Coexpressed VLP NES

    Plasmid

    #182433

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20 (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS
    deltaVP1
    VP2C-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1, GAL10, and GAL7

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Coexpressed VLP NES (M)

    Plasmid

    #182435

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96W-N127W) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS(M)
    deltaVP1
    VP2C-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1, GAL10, and GAL7

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Coexpressed VLP NES (G)

    Plasmid

    #182477

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96C) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS(G)
    deltaVP1
    VP2C-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1, GAL10, and GAL7

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Coexpressed Free PcPTS

    Plasmid

    #182483

    Purpose

    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and patchoulol synthase from Pogostemon cablin (PcPTS; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Inserts

    FPPS
    PTS
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL10 and GAL7

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Linked Free NES (AG4TGGA)2

    Plasmid

    #182488

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence (AG4TGGA)2 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Insert

    FPPS-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL10

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Linked Free (PT)4P

    Plasmid

    #182490

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence PTPTPTPTP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Insert

    FPPS-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL10

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGST2-GST-TEV-OPTN S177D S473D

    Plasmid

    #187827

    Purpose

    Plasmid for the expression and purification of GST-TEV-OPTN S177D S473D. Internal Ref: SMC1588

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    OPTN (OPTN Human)
    

    Tags

    GST-TEV

    Expression

    Bacterial

    Mutation

    S177D S473D

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAP14

    Plasmid

    #186262

    Purpose

    wgaA (a.k.a expA1) and wgaB (a.k.a expA23), bicistronic, under light light responsive PEL222 promoter and EL222 under constitutively active LacIq promoter

    Depositor

    Yongku Cho

    Article

    Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

    Inserts

    wgaAB bicistronic
    EL222 (ELI_04755 Erythrobacter litoralis)
    

    Expression

    Bacterial

    Promoter

    LacIq (CGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCC…

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLEX307_PDHA2S291D

    Plasmid

    #115193

    Purpose

    Lentiviral transduction and expression of PDHA2S291D into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    PDHA2S291D (PDHA2 Human)
    

    Use

    Lentiviral

    Mutation

    p.S291D

    Promoter

    EF1alpha

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLEX307_PDHA2S293D

    Plasmid

    #115194

    Purpose

    Lentiviral transduction and expression of PDHA2S293D into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    PDHA2S293D (PDHA2 Human)
    

    Use

    Lentiviral

    Mutation

    p.S293D

    Promoter

    EF1alpha

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLEX307_PDHA2S291D/S293D

    Plasmid

    #115195

    Purpose

    Lentiviral transduction and expression of PDHA2S291D/S293D into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    PDHA2S291D/S293D (PDHA2 Human)
    

    Use

    Lentiviral

    Mutation

    p.S291D/S293D

    Promoter

    EF1alpha

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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