19,660 results

1. pLenti CMV/TO Puro DEST (670-1)

   Plasmid

   #17293

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Puro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PB-gRNA-Puro

   Plasmid

   #121121

   Purpose

   Expression of a gRNA together with a Puromycin resistance

   Depositor

   Pablo Navarro

   Article

   Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

   Insert

   gRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3 LIC cloning vector (6A)

   Plasmid

   #30124

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Oct. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. c-myc promoter (Del 1)

   Plasmid

   #16601

   Depositor

   Bert Vogelstein

   Article

   He et al Science. 1998 Sep 4. 281(5382):1509-12.

   Insert

   c-myc promoter (Del 1) (MYC Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Del-1 (XmnI->PvuII)

   Available Since

   March 24, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLentiCMV Puro DEST ERKKTRClover

   Plasmid

   #59150

   Purpose

   Lentiviral vector to express ERK KTR mClover under CMV promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   ERK Kinase Translocation Reporter
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCR3.1-mGM-CSF

   Plasmid

   #74465

   Purpose

   expression of mGM-CSF in mammalian cells

   Depositor

   Loree Heller

   Article

   Heller et al Melanoma Res. 2000 Dec;10(6):577-83.

   Insert

   granulocyte-monocyte colony-stimulating factor (Csf2 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRSET FLIPPi-30m

   Plasmid

   #13556

   Depositor

   Wolf Frommer

   Article

   Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

   Insert

   FLIPPi T22A
   

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   T22A in binding protein

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKLV2-EF1a-BsdCas9-W

   Plasmid

   #67978

   Purpose

   Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   EF1a-Cas9 cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Cas9 fused with Flag-tag and a NLS at the N termi…

   Promoter

   EF1a

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBbS5a-Opto-Cre-Vvd-2

   Plasmid

   #160400

   Purpose

   Updated light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.

   Depositor

   Mary Dunlop

   Article

   Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

   Insert

   Opto-Cre-Vvd-2
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Bacterial

   Promoter

   lacUV5

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMXs-Tcl1

    Plasmid

    #13364

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    T-cell lymphoma breakpoint 1 (Tcl1 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pRDA_426

    Plasmid

    #179097

    Purpose

    BE Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    ABE8e
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-C1-TBP

    Plasmid

    #26674

    Depositor

    Sui Huang

    Article

    Chen et al Mol Biol Cell. 2002 Jan . 13(1):276-84.

    Insert

    TBP (TBP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTH2

    Plasmid

    #84452

    Purpose

    shuttle plasmid for sarA P1-CFP expression

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    CFP Cerulean
    

    Use

    Shuttle vector e.coli-s.aureus, expression of sgf…

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMRBad-Z-CspGFP

    Plasmid

    #40730

    Depositor

    Brian McNaughton

    Article

    Blakeley et al Mol Biosyst. 2012 Aug;8(8):2036-40. Epub

    Insert

    Leucine Zipper prime - C super positive Green Fluorescent Protein
    

    Expression

    Bacterial

    Promoter

    pBad

    Available Since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-Roxed-Cre

    Plasmid

    #51273

    Purpose

    Component of the Co-Driver binary site-specific recombinase system, generates functional rox60-71Cre upon removal of an internal STOP cassette by Dre

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    Roxed-Cre
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mApple-LAMP1-phLuorin-N-8

    Plasmid

    #54918

    Purpose

    Localization: Lysosome Membrane, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    LAMP1 phLuorin
    

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Lysosomes-20 double labeled with phLuorin

    Promoter

    CMV

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Beta-2-adrenergic receptor-CFP

    Plasmid

    #55794

    Purpose

    Beta-2-adrenergic receptor tagged with ECFP on carboxyl terminus

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    Beta-2-adrenergic receptor-ECFP (ADRB2 Aequorea victoria, Human)
    

    Tags

    ECFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRF-TetR

    Plasmid

    #49374

    Depositor

    Christopher Voigt

    Article

    Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

    Insert

    TetR repressor
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid

    #139996

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHBS1389 IBB-GFP-mCherry3E

    Plasmid

    #118803

    Purpose

    Fluorescence-based TDP43-dependent splicing reporter

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    Synthetic
    

    Expression

    Mammalian

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge