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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,757 results for: sequence

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Showing: 1051 - 1080 of 13757 results

  2. pMM441

    Plasmid
    #61271
    Purpose
    Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.
    Depositor
    Timothy Lu
    Article
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Available Since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    61271-plasmid-map-sequence-id-99582

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  4. FRB_LD 0_CS G_tTA-BFP

    Plasmid
    #58876
    Purpose
    MESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    58876-plasmid-map-sequence-id-99612

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  6. pBlueScript-humanAPOBEC1

    Plasmid
    #134606
    Purpose
    Plasmid bearing APOBEC1 coding sequence. In presence of its cofactor and overexpressing an artificial reporter with editable CAA codon in APOB sequence we can visualize its editing activity
    Depositor
    Silvestro Conticello
    Article
    Chieca et al bioRxiv 2021
    Insert
    humanAPOBEC1 (APOBEC1 Human)
    Expression
    Mammalian
    Promoter
    Beta actin
    Available Since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    134606-plasmid-map-sequence-id-258758

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  7. dTomato_LD 0_CS M_tTA-BFP

    Plasmid
    #58875
    Purpose
    MESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58875-plasmid-map-sequence-id-94236

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  11. pUC_sgRNA

    Plasmid
    #68710
    Purpose
    Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Available Since
    Sept. 14, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    68710-plasmid-map-sequence-id-123992

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  27. dTomato_LD 0_CS G_tTA-BFP

    Plasmid
    #58871
    Purpose
    MESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58871-plasmid-map-sequence-id-94232

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  29. mCherry_LD 0_CS G_tTA-BFP

    Plasmid
    #58856
    Purpose
    MESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58856-plasmid-map-sequence-id-94228

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Showing: 1051 - 1080 of 13757 results