We narrowed to 4,275 results for: Pet2

1. pET28a-Cys-ELP(120nm)-LPETGG

   Plasmid

   #90472

   Purpose

   Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   Cys-ELP(120nm)-LPETGG
   

   Use

   Tags

   Cysteine and Sortase-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a-Fgß-ddFLN4-HIS-ybbr

   Plasmid

   #101239

   Purpose

   wildtype Fgß peptide with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

   Depositor

   Hermann Gaub

   Article

   Milles et al Science. 2018 Mar 30;359(6383):1527-1533

   Insert

   Fgß peptide, ddFLN4 fingerprint (FGB Human, D. discoideum)
   

   Use

   Tags

   HIS and ybbr

   Expression

   Bacterial

   Mutation

   C18S in ddFLN4

   Promoter

   T7

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-CohIII-CBM-HIS-LPETGG

   Plasmid

   #91697

   Purpose

   CohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGG

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   CohIII-CBM-HIS-LPETGG
   

   Use

   Tags

   HIS-Tag and Sortase-Tag

   Expression

   Bacterial

   Mutation

   Cysteine deletion in CBM C63S

   Promoter

   T7

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a-mScarlet-TEV-Strep-His

   Plasmid

   #210823

   Purpose

   Bacterial overexpression vector for C-terminally Strep and His tagged mScarlet fluorescent protein

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   mScarlet
   

   Use

   Tags

   TwinStrep, 10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAM354 pET28a-GGG-Titin[V15P]-23-K-19-C-15

   Plasmid

   #226350

   Purpose

   Expression of proteasome substrate with tail for molecular ruler

   Depositor

   Andreas Martin

   Article

   Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

   Insert

   titin (TTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V15P

   Promoter

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-His10-SHP1_PTPase (243-541aa)

   Plasmid

   #180812

   Purpose

   Expression of 10*His tagged human SHP1 phosphatase domain (243-541aa)

   Depositor

   Enfu Hui

   Article

   Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   SHP1_PTPase (243-541aa) (PTPN6 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET21-Streptavidin-Dead Aspartate Loop

   Plasmid

   #46368

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

   Insert

   Streptavidin-Dead Aspartate Loop
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

8. Ana GvpC-SpyTag-His in pET28a

   Plasmid

   #85737

   Purpose

   Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

   Insert

   Gas vesicle protein C (fused to SpyTag and His-tagged)
   

   Use

   Tags

   His-tag and SpyTag

   Expression

   Bacterial

   Mutation

   Added an extra glycine after the methionine at th…

   Promoter

   T7

   Available Since

   March 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET29b-eGFP-dspB(E184Q)-6xHis

   Plasmid

   #175783

   Purpose

   Plasmid for overexpression of recombinant His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

   Insert

   Dispersin B
   

   Use

   Tags

   Hexahistidine tag and eGFP

   Expression

   Bacterial

   Mutation

   E184Q

   Promoter

   T7

   Available Since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET29b-mVenus-dspB(E184Q)-6xHis

    Plasmid

    #176576

    Purpose

    Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Use

    Tags

    Hexahistidine tag and mVenus

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-ybbR-HIS-Xyl-DocI

    Plasmid

    #58711

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    Xyl-DocI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    changed Threonin 120 to Cysteine in Xylanase Doma…

    Promoter

    T7

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-ybbR-HIS-sfGFP-DocI

    Plasmid

    #58708

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    sfGFP-DocI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pF712-pET28a-Hs-delta44-GCDH-NHis wt

    Plasmid

    #85113

    Purpose

    expression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta44) GCDH (GCDH Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    silent SNP: G1041T

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21-10XHis-GST-HRV-dHer1cys

    Plasmid

    #73218

    Purpose

    Bacterial expression vector for Z1907 affibody protein (A) with N-terminal His10, GST and HRV3C cleavage site (cysteine tag)

    Depositor

    Marcel Bruchez

    Article

    Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44

    Insert

    10XHis-GST-HRV-dHer1cys
    

    Use

    Tags

    10XHis-GST-HRV and The dimer of the Her1 Affibody…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET29-hMIGA2(170-593)-6xhis

    Plasmid

    #192834

    Purpose

    Express codon-optimized human MIGA2 fragment 170-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-RBP-GFP(S45C)-ZdK1

    Plasmid

    #185791

    Purpose

    Encodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linker

    Depositor

    Lizhong He

    Article

    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

    Insert

    RBP-sfGFP(S45C)-ZdK1
    

    Use

    Tags

    10xHis, TEV protease cleavage site after His Tag

    Expression

    Bacterial

    Mutation

    Ser to Cys mutation at start of GFP, residue 45 i…

    Promoter

    T7

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28-His8-aCat(660-906)

    Plasmid

    #203333

    Purpose

    Bacterial expression of alpha-catenin F-actin binding domain

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Use

    Tags

    8xHis with precision protease cleavage site

    Expression

    Bacterial

    Mutation

    aa 660-906 only

    Promoter

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

    Plasmid

    #124660

    Purpose

    reporter protein which emits bright blue fluorescence

    Depositor

    Yutaka Kuroda

    Article

    Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1

    Insert

    Gaussia luciferase-C9D-TGinsert
    

    Use

    Tags

    IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…

    Expression

    Bacterial

    Mutation

    changed glutamic acid 100 to alanine, and glycine…

    Promoter

    T7 promoter

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-ybbr-HIS-ddFLN4-K10

    Plasmid

    #101718

    Purpose

    K10 peptide with ddFLN4 fingerprint domain and N-terminal ybbr tag for covalent immobilization - resulting in high forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    ddFLN4 fingerprint, K10 peptide (KRT10 Human, Dictyostelium discoideum)
    

    Use

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Arabidopsis thaliana Atm3 d80 pET21a+

    Plasmid

    #173045

    Purpose

    Expresses AtAtm3 in E.coli

    Depositor

    Douglas Rees

    Article

    Fan et al Elife. 2022 Mar 25;11. pii: 76140. doi:

    Insert

    Arabidopsis thaliana Atm3 (ABCB25 Mustard Weed)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    with N-terminal 80 amino acids deletion

    Promoter

    Available Since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET23A-Fucosidase-E1_10125-Ruminococcus_gnavus-His

    Plasmid

    #207665

    Purpose

    Expresses fucosidase E1_10125 from Ruminococcus gnavus in BL21 cells, purified using His-tag

    Depositor

    Robert de Vries

    Article

    Spruit et al bioRxiv 2023.12.15.571923

    Insert

    Fucosidase E1_10125
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    amino acids 34-584 were used

    Promoter

    T7 promoter

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pF711-pET28a-Hs-delta25-MCAD-NHis wt

    Plasmid

    #85112

    Purpose

    expression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta25) MCAD (ACADM Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pF708-pET28a-Hs-delta38-METTL20-NHis wt

    Plasmid

    #85109

    Purpose

    expression of recombinant human mature (delta38) METTL20 wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta38) METTL20 (METTL20 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET21b-LOV-ipaA L514K L531E

    Plasmid

    #40253

    Depositor

    Brian Kuhlman

    Article

    Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.

    Insert

    LOV-ipaA L514K L531E
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L514K; L531E; Jα helix region hybridized (AsLOV2 …

    Promoter

    T7

    Available Since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pET28a-ybbr-HIS-ddFLN4-Fgß

    Plasmid

    #101719

    Purpose

    wildtype Fgß peptide with ddFLN4 fingerprint domain with N-terminal ybbr tag for covalent immobilization - resulting in weak forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    ddFLN4 fingerprint, Fgß peptide (FGB Human, Dictyostelium discoideum)
    

    Use

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. BPV00605 pET21GG2-His(6)-BECN(1-265)

    Plasmid

    #136686

    Purpose

    Expresses the Beclin-1 IDR+CC domains (a.a. 1-265) with a N-terminal hexa-Histidine tag

    Depositor

    Colin Garvie

    Article

    Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

    Insert

    Beclin-1 IDR + CC (BECN1 Human)
    

    Use

    Tags

    Hexa-Histadine

    Expression

    Bacterial

    Mutation

    DNA optimized for E. coli expression

    Promoter

    T7

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FL MALT1 Catalytic Mutant in pET29b-Strep

    Plasmid

    #48969

    Purpose

    Expresses Full length catalytic mutant MALT1 in e.coli

    Depositor

    Guy Salvesen

    Article

    Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

    Insert

    MALT1 isoform A (MALT1 Human)
    

    Use

    Tags

    His and Strep

    Expression

    Bacterial

    Mutation

    Catalytic Mutant C464A

    Promoter

    T7

    Available Since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

28. pET29-hMIGA2(306-593)-6xhis

    Plasmid

    #192837

    Purpose

    Express codon-optimized human MIGA2 fragment 306-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET22b-ecDHFR-N23PP/L28F/G51PEKN

    Plasmid

    #118585

    Purpose

    Bacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Insert

    DHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N23 mutated to P23, L28 mutated to F28, G51 mutat…

    Promoter

    T7

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28a SUMO Nup53xl 162-267

    Plasmid

    #86002

    Purpose

    bacterial expression of his6 and SUMO tagged RRM motif of Xenopus laevis Nup53

    Depositor

    Wolfram Antonin

    Type

    Empty backbone

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28a-FgßF3-ddFLN4-HIS-ybbr

    Plasmid

    #101743

    Purpose

    mutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    FgßF3 peptide, ddFLN4 fingerprint (FGB Human, Dictyostelium discoideum)
    

    Use

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4, added 9F in FgßF3

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. delNC C-His Ana GvpC in pET28a

    Plasmid

    #85736

    Purpose

    Expresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

    Insert

    Gas vesicle protein C (truncated and his-tagged)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Deletion of N and C terminal regions, addition of…

    Promoter

    T7

    Available Since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET28a-ybbR-HIS-CBM-CohI

    Plasmid

    #58709

    Purpose

    Probing molecule to perform single molecule force spectroscopy experiments

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    CBM-CohI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    changed Alanine 2 to Cysteine in CBM domain

    Promoter

    T7

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28b-NmCas9-Flag D16A+H588A

    Plasmid

    #109033

    Purpose

    D16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Use

    Tags

    Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET28a-Hook3 I136A, I139A, M142A

    Plasmid

    #74617

    Purpose

    Expresses a triple mutant truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    D102A, E108A

    Promoter

    T7

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET28b(+)_S. cerevisiae Hbs1(166-611)

    Plasmid

    #39338

    Depositor

    SeWon Suh

    Insert

    Elongation factor 1 alpha-like protein
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

37. pET28a-ybbR-HIS-10FNIII(x4)-DocI

    Plasmid

    #58712

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    10FNIII(x4)-DocI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28a-His6-GFP-UROD-N367A

    Plasmid

    #236742

    Purpose

    Protein overexpression of eGFP-human uroporphyrinogen decarboxylase N367A

    Depositor

    Christina Woo

    Article

    Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

    Insert

    eGFP-Uroporphyrinogen decarboxylase human N367A (UROD Human, Synthetic)
    

    Use

    Tags

    His6 and eGFP

    Expression

    Bacterial

    Mutation

    N367A

    Promoter

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET21a asyn(Δ41-54/Δ103-130)

    Plasmid

    #242236

    Purpose

    Expresses alpha synuclein (missing exon 3 and 5, residues 41-54 and 103-130) in bacteria

    Depositor

    Jennifer Lee

    Article

    SanGiovanni et al Protein Sci. 2025 Jul;34(7):e70195. doi:

    Insert

    asyn(Δ41-54/Δ103-130)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    deleted amino acids 41-54 and 103-130

    Promoter

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET21a asyn(66-140) K96A/K97A

    Plasmid

    #240590

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, K96A, K97A

    Promoter

    T7

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET21a asyn(66-140) E104A/E105A

    Plasmid

    #240589

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, E104A, E105A

    Promoter

    T7

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET21a asyn(66-140) E83K/K97E

    Plasmid

    #240591

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, E83K, K97E

    Promoter

    T7

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET21a asyn(66-140) K80E/E83K

    Plasmid

    #240593

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, E83K, K80E

    Promoter

    T7

    Available Since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET21a asyn(66-140) E83K/K96E

    Plasmid

    #240592

    Purpose

    Overexpress truncated alpha-Synuclein protein

    Depositor

    Jennifer Lee

    Article

    McGlinchey et al Nat Commun. 2025 Apr 23;16(1):3825. doi:

    Insert

    aSyn (SNCA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 66-140 only, E83K, K96E

    Promoter

    T7

    Available Since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pET21b(+)_SARS-CoV-2_3CLpro_Q306A-HIS

    Plasmid

    #224697

    Purpose

    Bacterial Expression and Purification of SARS-CoV-2 3CLpro active protease with His-tag

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    

    Use

    Tags

    Gly-Gly-6xHis

    Expression

    Bacterial

    Mutation

    silent mutation C to T at position 10546 (elimina…

    Promoter

    T7

    Available Since

    July 2, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

46. pET21b(+)_SARS-CoV-2_3CLpro_C145A-HIS

    Plasmid

    #224698

    Purpose

    Bacterial Expression and Purification of SARS-CoV-2 3CLpro inactive protease with His-tag

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    

    Use

    Tags

    Gly-Gly-6xHis

    Expression

    Bacterial

    Mutation

    silent mutation C to T at position 10546 (elimina…

    Promoter

    T7

    Available Since

    July 2, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

47. ECMAB - pET22b Ag43 160N 6H Mms6

    Plasmid

    #225539

    Purpose

    Construction of extracellular magnetite accumulating bacterial systems.

    Depositor

    Urartu Seker

    Article

    Yavuz et al Small. 2022 Jul;18(26):e2200537. doi: 10

    Inserts

    Iron binding protein
    Autotransporter protein
    

    Use

    Tags

    no

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pET22b Ag43 160N 6H mCherry-R5 AmpR

    Plasmid

    #225141

    Purpose

    Recombinant Ag43 protein fused to silaffin-R5 and mCherry. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.

    Depositor

    Urartu Seker

    Article

    Kirpat Konak et al Mater Today Bio. 2022 Oct 10;17:100461.

    Insert

    Ag43 160N, mCherry
    

    Use

    Tags

    Ag43 protein fused to silaffin-R5 and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pET28b-SUMO-FLAG-TeKaiA-FLAG

    Plasmid

    #229220

    Purpose

    For expression of T. elongatus KaiA protein

    Depositor

    Andy LiWang

    Insert

    kaiA (kaiA T. elongatus)
    

    Use

    Tags

    FLAG and SUMO-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pET28b-SUMO-FLAG-TeKaiB-FLAG

    Plasmid

    #229221

    Purpose

    For expression of T. elongatus KaiB protein

    Depositor

    Andy LiWang

    Insert

    kaiB (kaiB T. elongatus)
    

    Use

    Tags

    FLAG and SUMO-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge