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Showing: 1751 - 1800 of 4184 results

  1. pET22b Ag43 160N 6H mCherry-R5 AmpR

    Plasmid
    #225141
    Purpose
    Recombinant Ag43 protein fused to silaffin-R5 and mCherry. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.
    Depositor
    Urartu Seker
    Article
    Kirpat Konak et al Mater Today Bio. 2022 Oct 10;17:100461.
    Insert
    Ag43 160N, mCherry
    Use
    Tags
    Ag43 protein fused to silaffin-R5 and mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    225141-plasmid-map-sequence-id-460500

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  2. pET28b-SUMO-FLAG-TeKaiA-FLAG

    Plasmid
    #229220
    Purpose
    For expression of T. elongatus KaiA protein
    Depositor
    Andy LiWang
    Insert
    kaiA (kaiA T. elongatus)
    Use
    Tags
    FLAG and SUMO-FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229220-plasmid-map-sequence-id-460016

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  3. pET28b-SUMO-FLAG-TeKaiB-FLAG

    Plasmid
    #229221
    Purpose
    For expression of T. elongatus KaiB protein
    Depositor
    Andy LiWang
    Insert
    kaiB (kaiB T. elongatus)
    Use
    Tags
    FLAG and SUMO-FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    229221-plasmid-map-sequence-id-457227

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  4. pET28b-SUMO-SeKaiB-K25C-FLAG

    Plasmid
    #229225
    Purpose
    For expression of S. elongatus KaiB protein mutant
    Depositor
    Andy LiWang
    Insert
    kaiB (kaiB S. elongatus)
    Use
    Tags
    FLAG and SUMO
    Expression
    Bacterial
    Mutation
    K25C
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    229225-plasmid-map-sequence-id-457071

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  8. pET28a-SdrG-B2-ELP-HIS-ybbR

    Plasmid
    #169134
    Purpose
    E. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.
    Depositor
    Michael Nash
    Article
    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi
    Insert
    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    Use
    Tags
    3xELP linker, 6x Histag, and ybbr tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    169134-plasmid-map-sequence-id-380322

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  11. pET28a-SdrG-B1-ELP-HIS-ybbR

    Plasmid
    #169133
    Purpose
    E. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.
    Depositor
    Michael Nash
    Article
    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi
    Insert
    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    Use
    Tags
    3xELP linker, 6x Histag, and ybbr tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    169133-plasmid-map-sequence-id-361153

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  16. pET28_6xHis-GAL4-PARP1-CAT-L713F

    Plasmid
    #175949
    Purpose
    Bacterial expression of a fusion of yeast GAL4 DNA binding domain and human PARP1 CAT domain containing L713F gain-of-function mutation
    Depositor
    Joan Conaway
    Article
    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118
    Insert
    GAL4 DNA binding domain (GAL4 Budding Yeast)
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    L713F, V762A for the 'PARP1-CAT-L713F' …
    Promoter
    T7
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    175949-plasmid-map-sequence-id-345352

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  18. pET28-H12TEV-hEIF5B-587~1220

    Plasmid
    #208637
    Purpose
    for E. coli expression truncated human eIF5B (ENST00000289371), aa587~1220
    Depositor
    Quanfu Ma
    Insert
    human eIF5B (ENST00000289371) aa 587~1220 (EIF5B Human)
    Use
    Tags
    6xhis
    Expression
    Bacterial
    Mutation
    contain SNP rs1376443, protein sequence not chang…
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208637-plasmid-map-sequence-id-433646

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  19. pET28-RPL3-1~41-mEGFP(A206K)-TwStrep

    Plasmid
    #208628
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa1~41, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 1~41 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 1~41, other residues not included
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208628-plasmid-map-sequence-id-433639

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  23. pET28-RPL3-Δ1~34,Δ215~284-TwStrep

    Plasmid
    #208630
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa35~398 with deletion of 215~284, fused with Twin Strep tag.
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) Δ1~34, Δ215~284 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    Δ1~34,Δ215~284
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208630-plasmid-map-sequence-id-418338

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  24. pET28-RPL3-53~77-mEGFP(A206K)-TwStrep

    Plasmid
    #208632
    Purpose
    for E. coli expression truncated human RPL3 (ENST00000216146), aa53~77, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 53~77 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 53~77, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208632-plasmid-map-sequence-id-418343

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  25. pET28-RPL3-1~77-mEGFP(A206K)-TwStrep

    Plasmid
    #208624
    Purpose
    for E. coli expression truncated human RPL3 (ENST00000216146), aa1~77, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 1~77 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 1~77, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208624-plasmid-map-sequence-id-418336

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  33. pET28a-FFSARG-ddFLN4-HIS-ybbr

    Plasmid
    #101240
    Purpose
    minimal SdrG binding peptide FFSARG with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces
    Depositor
    Hermann Gaub
    Article
    Milles et al Science. 2018 Mar 30;359(6383):1527-1533
    Insert
    FFSARG peptide, ddFLN4 fingerprint
    Use
    Tags
    HIS and ybbr
    Expression
    Bacterial
    Mutation
    C18S in ddFLN4
    Promoter
    T7
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    101240-plasmid-map-sequence-id-199090

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  37. pET28b(+)_S. cerevisiae Hbs1(162-611)

    Plasmid
    #39337
    Depositor
    SeWon Suh
    Insert
    Elongation factor 1 alpha-like protein
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 27, 2012
    Availability
    Academic Institutions and Nonprofits only

  45. pET28-SpRY-HF1-NLS-6xHis (gbr2102)

    Plasmid
    #181744
    Purpose
    bacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    bacterial codon optimized SpRY-HF1
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS(SV40)-6xHis and MKIEE MBP tag
    Expression
    Bacterial
    Mutation
    SpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…
    Promoter
    T7
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    181744-plasmid-map-sequence-id-373171

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  47. pET28a 6xHis PreScission SARS-CoV-2 nsp13

    Plasmid
    #159390
    Purpose
    Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.
    Depositor
    Tarun Kapoor
    Article
    Chen et al bioRxiv. 2020 Jul 13. doi: 10.1101/2020.
    Insert
    N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    159390-plasmid-map-sequence-id-310625

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Showing: 1751 - 1800 of 4184 results