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Plasmid#215342PurposeRecombineering plasmid with constitutively expressed cas9 and the araBAD promoter expressing αβγ on a BBR1 backboneDepositorInsertCas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23105Available sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Plac-dCas9 (Plac)
Plasmid#105235PurposeTn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Plac promoter for expression in P. aeruginosaDepositorInsertS. pasteurianus dCas9
UseCRISPR and Synthetic BiologyTagsHAExpressionBacterialMutationD10A, H599APromoterAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB229
Plasmid#68620PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagstdTomatoExpressionPlantMutationPromoterAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-XRCC6
Plasmid#192302PurposeGateway entry vector for an inducible 3xFLAG-XRCC6DepositorInsertXRCC6 (XRCC6 Human)
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1 PIK3R1
Plasmid#17743DepositorInsertPIK3R1 (PIK3R1 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKanB alpha
Plasmid#223524PurposeUsed for expression of secreted recombinant protein in Pichia pastoris; with G418 resistance gene as selectable markerDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAOX1Available sinceSept. 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBIG2abc
Plasmid#80617PurposepBIG2abc - Step2 vector abc (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBJ1
Plasmid#167130PurposeA gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoterDepositorInsertluxCDABE
UseLuciferaseTagsExpressionBacterialMutationPromoterFrr promoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG2abcde
Plasmid#80619PurposepBIG2abcde - Step2 vector abcde (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG2abcd
Plasmid#80618PurposepBIG2abcd - Step2 vector abcd (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG1c
Plasmid#80613PurposepBIG1c - Step1 vector c (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG1d
Plasmid#80614PurposepBIG1d - Step1 vector d (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEx-deletion-tet
Plasmid#172214PurposeFor allelic exchange in tetracyclin-sensitive Prevotella copri strainsDepositorTypeEmpty backboneUseAllelic exchange in prevotella copriTagsExpressionMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBIG1e
Plasmid#80615PurposepBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
CyTERM-mGFP
Plasmid#62237PurposeER targeted transmembrane domain with monomeric EGFP oriented towards the cytoplasm. Used for assessing fluorescent protein oligomerization.DepositorInsertCyTERM-mGFP
UseTagsmGFPExpressionMammalianMutationsignal anchor of rabbit cytochrome p450 and monom…PromoterCMVAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBJ6
Plasmid#167135PurposeA gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoterDepositorInsertluxCDABE
UseLuciferaseTagsExpressionBacterialMutationPromoterKanamycin promoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBJ5
Plasmid#167134PurposeA gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoterDepositorInsertluxCDABE
UseLuciferaseTagsExpressionBacterialMutationPromoterFrr promoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJB158
Plasmid#202601PurposeRP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual
Plasmid#216377PurposeBaculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)DepositorInsertLDL receptor related protein 6 (LRP6 Human)
UseBaculovirusTags9xHis and HA signal sequence-FLAG-3C cleavage sit…ExpressionInsectMutationPromoterPolyhedrin (LRP6)/p10 (MESD)Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
MX01
Plasmid#158624PurposeGATEWAY and BacMam compatible mammalian expression vector with eGFP expression reporter (eGFP is expressed by same promotor but as a separate polypeptide chain).DepositorTypeEmpty backboneUseGateway destination vector ; bacmam acceptor vect…TagsP2AExpressionMammalianMutationPromoterCMV-IEAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only