We narrowed to 747 results for: PI

1. pET41a iGlul

   Plasmid

   #119832

   Purpose

   Production of recombinant iGluSnFR T92A low affinity variant, a single-wavelength glutamate indicator, in E. coli

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGlul
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   T92A

   Promoter

   T7

   Available since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET41a iGluSnFR E25R

   Plasmid

   #119831

   Purpose

   Production of recombinant iGluSnFR E25R variant, a single-wavelength glutamate indicator, in E. coli

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluSnFR E25R
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   E25R

   Promoter

   T7

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pZS1[GalS-L,LacI-L]

   Plasmid

   #60759

   Purpose

   Contains PIq driving expression GalS-L, and PI driving expression of LacI-L.

   Depositor

   Matthew Bennett

   Article

   Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

   Inserts

   GalS-L
   wt LacI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   LacI/GalR repressor chimera. LacI DBD with GalS L…

   Promoter

   Available since

   July 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET41a iGlum

   Plasmid

   #119829

   Purpose

   Production of recombinant iGluSnFR R24K medium affinity variant, a single-wavelength glutamate indicator, in E. coli

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGlum
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   R24K

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PDIA3-bio-His

   Plasmid

   #52070

   Purpose

   Expresses full-length Protein disulfide-isomerase A3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PDIA3 (PDIA3 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pATZ-HA-GFP11

   Plasmid

   #177720

   Purpose

   ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to an HA tag and the eleventh β-strand of GFP

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   alpha-1-antitrypsin (SERPINA1 Human)
   

   Use

   Tags

   GFP11 and HA

   Expression

   Bacterial and Mammalian

   Mutation

   E342K (ATZ mutant)

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAT-WT-HA-GFP11

   Plasmid

   #177719

   Purpose

   ER protein alpha-1-antitrypsin (WT) fused to an HA tag and the eleventh β-strand of GFP

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   alpha-1-antitrypsin (SERPINA1 Human)
   

   Use

   Tags

   GFP11 and HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mKate2-P2A-APEX2-ML1N*2

   Plasmid

   #67665

   Purpose

   Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain, [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   mKate2-P2A-APEX2-ML1N*2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV iGluu

   Plasmid

   #105832

   Purpose

   Fast indicator for extracellular glutamate imaging

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluSnFR S72T variant
   

   Use

   Tags

   Myc and PDGFR

   Expression

   Mammalian

   Mutation

   Changed Ser 72 to Thr

   Promoter

   CMV

   Available since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV iGluf

    Plasmid

    #105833

    Purpose

    Fast indicator for extracellular glutamate imaging

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR E25D variant
    

    Use

    Tags

    Myc and PDGFR

    Expression

    Mammalian

    Mutation

    Changed Glu 25 to Asp

    Promoter

    CMV

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. BTK-APEX2-P2A-mKate2

    Plasmid

    #67653

    Purpose

    Mammalian expression vector driving BTK-APEX2 (Human BTK-PH domain, [marks PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    BTK-PH-APEX2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pegAAT K342E correction

    Plasmid

    #169841

    Purpose

    Express a pegRNA used for correction (via A•T-to-G•C) of the E342K mutation

    Depositor

    Wen Xue

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    pegAAT K342E correction (SERPINA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRSFDuet-1-Cdc45

    Plasmid

    #208796

    Purpose

    Xenopus laevis FLAG5-His10-TEV-Cdc45

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    Cdc45 (cdc45.L Frog)
    

    Use

    Tags

    FLAG and His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSFDuet-1-Cdc45-6His

    Plasmid

    #211379

    Purpose

    bacterial expression of human Cdc45 C-terminally fused to 6xHis-Tag

    Depositor

    Helmut Pospiech

    Article

    Szambowska et al Nucleic Acids Res. 2014 Feb;42(4):2308-1

    Insert

    Cdc45 (CDC45 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. sgAAT Nicking

    Plasmid

    #169842

    Purpose

    Express a nicking sgRNA used for correction (via A•T-to-G•C) of the E342K mutation

    Depositor

    Wen Xue

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    sgAAT Nicking (SERPINA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pZS1[RbsR-L,LacI-L]

    Plasmid

    #60757

    Purpose

    Contains PIq driving expression RbsR-L, and PI driving expression of LacI-L.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Inserts

    RbsR-L
    Dimeric LacI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    LacI/GalR repressor chimera. LacI DBD with RbsR L…

    Promoter

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV-P(Cry1)-DIO-intron336-dLUC

    Plasmid

    #110056

    Purpose

    Cry1 transcription luciferase reporter

    Depositor

    Eric Erquan Zhang

    Article

    Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

    Insert

    Cry1 promoter and luciferase (Cry1 Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cry1

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-P(Cry1)-forward-intron336-Venus-NLS-D2

    Plasmid

    #110054

    Purpose

    Cry1 transcription fluorescence reporter

    Depositor

    Eric Erquan Zhang

    Article

    Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

    Insert

    Cry1 promoter and Venus (Cry1 Mouse)
    

    Use

    AAV

    Tags

    NLS-D2

    Expression

    Mammalian

    Mutation

    Promoter

    Cry1

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mKate2-P2A-APEX2-TAPP1-PH

    Plasmid

    #67662

    Purpose

    Mammalian expression vector driving APEX2-TAPP1 (Human TAPP1-PH domain, [marks PI(3,4)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-TAPP1-PH
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA_MET_D14_3Flag

    Plasmid

    #182495

    Purpose

    Contains cDNA of the MET gene without the exon 14

    Depositor

    Paolo Comoglio

    Article

    Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

    Insert

    MET (MET Human)
    

    Use

    Tags

    C-terminal 3xFlag tag

    Expression

    Mammalian

    Mutation

    Exon 14 deleted

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge