We narrowed to 24,925 results for: crispr

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pET-Himar-dCas9-mammalian

Plasmid

#138414

Purpose

Protein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tag

Depositor

Harris Wang

Publication

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Tags

3xFLAG, 6xHis, and SV40 NLS

Expression

Bacterial

Promoter

T7

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-BsCas12b-2AeGFP

Plasmid

#121948

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Publication

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BsCas12b

Tags

3xHA tag

Expression

Mammalian

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos3_AmpR

Plasmid

#119146

Purpose

Encodes sgRNA targeting position 3 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Publication

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA targeting position 3 in pColE1_70a_deGFP_KanR

Use

Crispr

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos7_AmpR

Plasmid

#119150

Purpose

Encodes sgRNA targeting position 7 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Publication

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA targeting position 7 in pColE1_70a_deGFP_KanR

Use

Crispr

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos5_AmpR

Plasmid

#119148

Purpose

Encodes sgRNA targeting position 5 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Publication

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA targeting position 5 in pColE1_70a_deGFP_KanR

Use

Crispr

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos2_AmpR

Plasmid

#119145

Purpose

Encodes sgRNA targeting position 2 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Publication

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA targeting position 2 in pColE1_70a_deGFP_KanR

Use

Crispr

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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PX458_NFIA_iso1_2

Plasmid

#104049

Purpose

Encodes gRNA for 3' target of human NFIA_iso1 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NFIA_iso1 gRNA (NFIA Human)

Use

CRISPR

Expression

Mammalian

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_NFIA_iso1_1

Plasmid

#104048

Purpose

Encodes gRNA for 3' target of human NFIA_iso1 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NFIA_iso1 gRNA (NFIA Human)

Use

CRISPR

Expression

Mammalian

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_RXRB_1

Plasmid

#104050

Purpose

Encodes gRNA for 3' target of human RXRB along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RXRB gRNA (RXRB Human)

Use

CRISPR

Expression

Mammalian

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pC026 - PpCas13a from Paludibacter propionicigenes WB4

Plasmid

#91916

Purpose

Expresses PpCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Publication

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

PpCas13a and guide expression cassette

Expression

Bacterial

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pC024 - CgCas13a from Carnobacterium gallinarum DSM 4847

Plasmid

#91914

Purpose

Expresses CgCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Publication

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

CgCas13a and guide expression cassette

Expression

Bacterial

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pC022 - LbnCas13a from Lachnospiraceae bacterium NK4A179

Plasmid

#91912

Purpose

Expresses LbnCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Publication

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LbnCas13a and guide expression cassette

Expression

Bacterial

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pC023 - CaCas13a from [Clostridium] aminophilum DSM 10710

Plasmid

#91913

Purpose

Expresses CaCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Publication

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

CaCas13a and guide expression cassette

Expression

Bacterial

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pC021 - LbmCas13a from Lachnospiraceae bacterium MA2020

Plasmid

#91911

Purpose

Expresses LbmCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Publication

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LbmCas13a and guide expression cassette

Expression

Bacterial

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_K1082A

Plasmid

#91863

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Publication

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) K1082A

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

K1082A

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_KLF9_2

Plasmid

#86347

Purpose

Encodes gRNA for 3' target of human KLF9

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA against KLF9 (KLF9 Human)

Use

CRISPR

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_KLF9_1

Plasmid

#86346

Purpose

Encodes gRNA for 3' target of human KLF9

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA against KLF9 (KLF9 Human)

Use

CRISPR

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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D. vulgaris Cas5c/pHMGWA

Plasmid

#81182

Purpose

Expresses Desulfovibrio vulgaris Cas5c in E. coli with TEV-cleavable N-terminal His-MBP tag

Depositor

Jennifer Doudna

Publication

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Insert

Cas5c

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Promoter

T7

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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D. vulgaris Cas8c/pHMGWA

Plasmid

#81183

Purpose

Expresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable N-terminal His-MBP tag

Depositor

Jennifer Doudna

Publication

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Insert

Cas8c

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Promoter

T7

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pX330.iGFP5

Plasmid

#66584

Purpose

sgRNA for iGFP

Depositor

Wen Xue

Publication

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

iGFP

Expression

Mammalian

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only