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pBluescript GPX-1

Plasmid

#13038

Depositor

Peter Newburger

Article

Shen et al J Biol Chem. 1993 May 25. 268(15):11463-

Insert

GPX-1 (GPX1 Human)

Expression

Bacterial

Mutation

Additional GCG upstream of codon 11. Codon 92 is …

Available Since

Nov. 7, 2006

Availability

Academic Institutions and Nonprofits only
pFUW-TetO-mCherry-cNLS

Plasmid

#138531

Purpose

Lentiviral inducible expression of mCherry with nuclear localization signal

Depositor

Christopher Grigsby, Ana Teixeira

Article

Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

mCherry

Use

Lentiviral

Tags

3x SV40 cNLS

Expression

Mammalian

Promoter

TRE-CMV

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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CYFIP2.mCherry

Plasmid

#122052

Purpose

Expresses human CYFIP2 in mammalian cells.

Depositor

Josef Kittler

Article

Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

Insert

CYFIP2 (CYFIP2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_OTmut

Plasmid

#185389

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin control sensor GRAB_OTmut

Use

AAV

Promoter

EFS

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pOP/SSAT

Plasmid

#140291

Purpose

Expresses human SSAT in bacterial cells

Depositor

Saverio Bettuzzi, Gaetano Marverti

Article

Marverti et al Carcinogenesis. 2005 Oct;26(10):1677-86.

Insert

SSAT1 (SAT1 Human)

Expression

Bacterial

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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FKBP-alpha(740-977)-GFP

Plasmid

#100731

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis (negative control)

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2A1 (Ap2a1 Mouse)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Long isoform

Promoter

CMV

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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BII-ChPtW-EGFP

Plasmid

#133400

Purpose

Piggybac vector for constitutive expression of EGFP

Depositor

Blair Madison

Article

Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

Insert

EGFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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PB-Gata2

Plasmid

#60665

Purpose

PiggyBac expression vector encoding rat Gata2 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Gata2 (Gata2 Rat)

Use

Piggybac

Expression

Mammalian

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1_UQCRC2

Plasmid

#177982

Purpose

lentiviral vector expressing Cas9 and a sgRNA targeting UQCRC2

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

sgRNA targeting UQCRC2 (UQCRC2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

U6

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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moPrP(3F4) D177N

Plasmid

#1319

Depositor

Susan Lindquist

Article

Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

Insert

PrP D177N (Prnp Mouse)

Expression

Mammalian

Mutation

D177N point mutation

Available Since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only
pMXs_FLAG-MCART2

Plasmid

#133250

Purpose

Express FLAG-tagged MCART2 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

MCART2 (SLC25A52 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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mSecPrP-pCB6+

Plasmid

#13917

Depositor

Susan Lindquist

Article

Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

Insert

PrP (Prnp Mouse)

Expression

Mammalian

Mutation

3F4 epitag

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pFGL1252_TetOFF(Hyg)

Plasmid

#118992

Purpose

TetOFF (promoter-less) cassette with Hygromycin selection

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Inserts

tTA+Tnos
Hygromycin cassette
Ttub+PtetO

Use

Fungal expression (in magnaporthe oryzae)

Mutation

Codon optimized for Magnaporthe oryzae

Promoter

Aspergillus nidulans TrpC promoter, Ustilago mayd…

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ptdTomato-N1-FLCN

Plasmid

#49174

Purpose

Mammalian expression of mouse folliculin (FLCN) fused to tdTomato

Depositor

Shawn Ferguson

Article

Petit et al J Cell Biol. 2013 Sep 30;202(7):1107-112

Insert

Folliculin (Flcn Mouse)

Tags

tdTomato

Expression

Mammalian

Mutation

Stop codon deleted

Promoter

CMV

Available Since

Nov. 26, 2013

Availability

Academic Institutions and Nonprofits only

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CALID

Plasmid

#169708

Purpose

Cre-activated light-inducible Dre recombinase

Depositor

Dali Li

Article

Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

Insert

a double-floxed inverted Dre246-nMag-T2A-pMag-Dre247

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-FNIP1

Plasmid

#49175

Purpose

Mammalian expression of mouse folliculin interacting protein 1 (FNIP1) fused to GFP

Depositor

Shawn Ferguson

Article

Petit et al J Cell Biol. 2013 Sep 30;202(7):1107-112

Insert

Folliculin Interacting Protein 1 (Fnip1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Stop codon deleted

Promoter

CMV

Available Since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_09

Plasmid

#194670

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_09 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_09

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-MCS-hPOT1_IRES-blast

Plasmid

#166411

Purpose

express hPOT1 in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

hPOT1 (POT1 Human)

Use

Lentiviral

Tags

myc

Expression

Mammalian

Promoter

ef-1a

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCW57.1_V5_mNeonGreen_FLAG_C

Plasmid

#218073

Purpose

RESOLUTE cloning plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Type

Empty backbone

Use

Lentiviral

Tags

FLAG-mNeonGreen-V5

Available Since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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pECFP-C1-hSERT

Plasmid

#70104

Purpose

mammalian expression of hSERT with N-terminal CFP fusion

Depositor

Harald Sitte

Article

Sucic et al J Biol Chem. 2010 Apr 2;285(14):10924-38

Insert

SERT (SLC6A4 Human)

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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