We narrowed to 26,489 results for: Vit;

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ABE8e(TadA-8e V82G)

Plasmid

#138494

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e V82G)-nSpCas9

Expression

Mammalian

Mutation

TadA-8e V82G

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-HA

Plasmid

#120902

Purpose

Expresses Ptch1-B for function assays

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

ptch1 (Ptch1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-hisG

Plasmid

#89954

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting hisG.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

hisG gRNA

Expression

Bacterial

Promoter

pTet

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 sgLacZ-1

Plasmid

#74968

Purpose

sgLacZ-1, puromycin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

LacZ

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 sgLacZ-2

Plasmid

#74969

Purpose

sgLacZ-2, puromycin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

LacZ

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pFLAG-4EHP(K121/130/134/222R)

Plasmid

#17353

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

4EHP (EIF4E2 Human)

Tags

Flag

Expression

Mammalian

Mutation

K121/130/134/222R

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only
pmCherry-Akt2(K14A)

Plasmid

#86624

Purpose

Expresses mCherry-tagged human Akt2(K14A) membrane binding mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2(K14A) (AKT2 )

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLLOXmPOLBeta

Plasmid

#24174

Depositor

Robert Sobol

Article

Jelezcova et al Mutat Res. 2010 Apr 1;686(1-2):57-67. do

Insert

pLLOXmPOLbeta (Polb Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

x

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only
pET-FLAG-eSpCas9

Plasmid

#126769

Purpose

Expression of increased fidelity eSpCas9 in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

eSpCas9

Use

CRISPR

Tags

3xFLAG, 6xHis, MBP, and NLS

Expression

Bacterial

Mutation

K848A, K1003A, R1060A

Promoter

T7

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)MBII-85-3'ss mut

Plasmid

#67644

Purpose

MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 3' splice site was mutated to consensus sequences.

Depositor

Stefan Stamm

Article

Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

Insert

MBII85 snoRNA

Expression

Mammalian

Mutation

3' splice site muattion

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR-GFP-Patronin CC 638–1457

Plasmid

#59051

Purpose

for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

eGFP

Expression

Bacterial and Insect

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pENTR-GFP-Patronin CC 776–1457

Plasmid

#59052

Purpose

for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

eGFP

Expression

Bacterial and Insect

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pKDsgRNA-trmI

Plasmid

#89955

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting trmI.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

trmI gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Shp2 SH2s

Plasmid

#67575

Purpose

Expresses GST-tagged Shp2 SH2 domains in bacteria

Depositor

Graeme Carnegie

Article

Burmeister et al J Biol Chem. 2015 May 8;290(19):12058-67

Insert

Src homology 2 domain-containing phosphatase 2 SH2s

Tags

GST

Expression

Bacterial

Mutation

Deleted amino acids 219-597

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-24-Decoy.hPGK.GFP.WPRE

Plasmid

#46602

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-24 Decoy (Mir24-1 Human, Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pSUPER.retro.puro-shMePCE

Plasmid

#113536

Purpose

Retroviral vector designed to knock down MePCE.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shMePCE (MEPCE Human)

Use

Retroviral

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-370-5p.sensor.PGK.dNGFR.WPRE

Plasmid

#85854

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-370-5p Sensor (MIR370 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pWJPrP5

Plasmid

#22106

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

mouse PrP ORF insert (Prnp Mouse)

Use

Construction of targeting vector

Mutation

mutated codon 177 (D to N)

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only
pKDsgRNA-pfkB

Plasmid

#102284

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting pfkB.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

pfkB gRNA

Use

CRISPR

Expression

Bacterial

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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vsv-Sgo1 N61I-GFP

Plasmid

#108497

Purpose

expression of vsv-Sgo1 N61I-EGFP (no PP2A binding)

Depositor

Susanne Lens

Article

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Insert

Shugoshin 1 (SGO1 Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

no PP2A binding N61I

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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