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pAAV-hsyn-GRAB_Ado1.0mut

Plasmid

#153287

Purpose

Express the adenosine-insensitive control sensor GRAB_Ado1.0mut in neurons

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Insert

Adenosine-insensitive sensor GRAB_Ado1.0mut (control sensor)

Use

AAV

Promoter

hSyn

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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Pol

Plasmid

#65520

Purpose

Expresses HBV polymerase in mammalian cells

Depositor

Wang-Shick Ryu

Article

Ryu et al Virology. 2008 Mar 30;373(1):112-23. Epu

Insert

HBV polymerase protein

Tags

Flag

Expression

Mammalian

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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MS2-p65-HSF1-P2A-mCherry

Plasmid

#184440

Purpose

MS2 coat protein fused with p65 and hsf1

Depositor

Rhys Allan

Article

Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

Insert

MS2-p65-hsf1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCGN-MEF2B

Plasmid

#32959

Purpose

For mammalian expression of human MEF2B with an HA tag

Depositor

Ron Prywes

Article

Han et al Mol Cell Biol. 1995 Jun;15(6):2907-15.

Insert

MEF2B (MEF2B Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pKO (Hyg)

Plasmid

#110087

Purpose

Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Tat101(AD8)-Flag

Plasmid

#115777

Purpose

pCMV-Tat101AD8-Flag allows the expression of Tat101(AD8) protein with a C-terminal flag-tag.

Depositor

Damian Purcell

Article

Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

Insert

HIV-1 Tat protein (AD8)

Tags

Flag

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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Rab5a-pmCherryC1

Plasmid

#27679

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Rab5a (Rab5a Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control

Plasmid

#128749

Purpose

Expresses control gRNA

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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LYS-Leu2

Plasmid

#218114

Purpose

Integration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGY199

Plasmid

#239801

Purpose

Express EGFP in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

EGFP

Use

Fungal expression

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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Integrin-β6 Headpiece I270C

Plasmid

#159638

Purpose

Contains integrin beta 6 residues 1–474 with I270C mutation

Depositor

Timothy Springer

Article

Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

Insert

Integrin beta 6 headpiece I270C

Tags

6x His

Expression

Mammalian

Mutation

I270C

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pTK_Sox10_enh_10E2_Cherry

Plasmid

#130590

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox10_enh_10E2

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Sox10_enh_84_Citrine

Plasmid

#130584

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox10_enh_84

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pJAT10

Plasmid

#204293

Purpose

For marking CRISPR HDR integrant with DsRed expressed in flight muscles.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-DsRed

Use

CRISPR

Promoter

MHC

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroSuper-shYes

Plasmid

#26987

Depositor

Joan Massague

Article

Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

Insert

shRNA Yes (YES1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pFRT_TO_FlagHA_TIAL1

Plasmid

#106090

Purpose

gene expression of FlagHA-TIAL1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

TIAL1 (TIAL1 Human)

Tags

FlagHA

Expression

Mammalian

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6c-IVT

Plasmid

#215835

Purpose

Template for in vitro transcription of CBE6c (with SpCas9)

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6c-SpCas9-2xUGI

Use

In vitro transcription template

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_mutCKAR3

Plasmid

#238414

Purpose

AAV construct with CKAR3 mutant expression under a human synapsin promotor and WPRE3 translation enhancer.

Depositor

Haining Zhong

Article

Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

Insert

phosphorylation deficient mutant of CKAR3 (T to A)

Use

AAV

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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BIN1-pmCherryN1

Plasmid

#27693

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

BIN1 (Bin1 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
WT WWP1

Plasmid

#200085

Purpose

WWP1 in pQCXIN vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

WT WWP1 (WWP1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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MPZ(P0)promoter pGL3-TATA

Plasmid

#21259

Depositor

Jerry Crabtree

Article

Kao et al Science. 2009 Jan 30. 323(5914):651-4.

Insert

myelin protein zero promoter (Mpz Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only

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Keratin 18 (pcDNA3)

Plasmid

#18064

Depositor

M Bishr Omary

Article

Ku et al J Cell Biol. 1994 Oct . 127(1):161-71.

Insert

Keratin 18 (KRT18 Human)

Expression

Mammalian

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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UIM-ECFP

Plasmid

#21067

Depositor

Xiaowei Zhuang

Article

Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

Insert

UIM (Epn1 Rat)

Tags

ECFP

Expression

Mammalian

Available Since

May 22, 2009

Availability

Academic Institutions and Nonprofits only
FCHo2-pmCherryC1

Plasmid

#27686

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

FCHo2 (Fcho2 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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DYRK1A

Plasmid

#38913

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

DYRK1A (DYRK1A Human)

Tags

His Tev

Expression

Bacterial

Mutation

codon-optimized

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
mg180

Plasmid

#34660

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A2;B0;C1) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pLVX_Venus

Plasmid

#247758

Purpose

Lentiviral expression of Venus in mammalian cells

Depositor

Alan Ashworth

Article

Abrams et al Cell Chem Biol. 2025 Nov 10:S2451-9456(2

Insert

Venus

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE50

Plasmid

#90389

Purpose

PPARg/RARa - PPARg gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

ACO1

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST/LIfeAct-mCherry-N1

Plasmid

#40908

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

LifeAct

Tags

mCherry

Expression

Mammalian

Mutation

No stop codon to create c-terminal fusion

Promoter

CMV

Available Since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA5_FRT_mCH

Plasmid

#127109

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

mCherry

Expression

Mammalian

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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