4,183 results

1. pMS2-YFP

   Plasmid

   #27122

   Depositor

   Robert Singer

   Article

   Fusco et al Curr Biol. 2003 Jan 21. 13(2):161-7.

   Insert

   MS2
   

   Tags

   EYFP, HA, and SV40 NLS

   Expression

   Mammalian

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-myc-Rac1-Q61L

   Plasmid

   #12983

   Depositor

   Gary Bokoch

   Insert

   Rac1 constitutively active (RAC1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Q61L Constitutively active

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. GST-Pin1

   Plasmid

   #19027

   Depositor

   Michael Yaffe

   Article

   Yaffe et al Science. 1997 Dec 12. 278(5345):1957-60.

   Insert

   Pin1 (PIN1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Aug. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO GFP HSPA8

   Plasmid

   #19487

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   HSPA8 (HSPA8 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

   Plasmid

   #42337

   Purpose

   Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmiRFP703-N1

   Plasmid

   #79988

   Purpose

   Monomeric near-infrared fluorescent protein miRFP703 in mammalian plasmid

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

   Insert

   miRFP703
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223 SARS-CoV-2 NSP16

   Plasmid

   #141269

   Purpose

   Gateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   April 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAAV-hDlx-GqDREADD-dTomato-Fishell-4

   Plasmid

   #83897

   Purpose

   Gq-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element

   Depositor

   Gordon Fishell

   Article

   Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   hM3Dq
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hDlx

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG414GPD-dCas9-VPR

   Plasmid

   #63801

   Purpose

   pAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vector

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Yeast

   Promoter

   GPD

   Available Since

   April 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSicoR-Ef1a-mCh-Puro

    Plasmid

    #31845

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression. Includes puromycin selection.

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available Since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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