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lenti-SpCas9 hygro
Plasmid#104995PurposeThis lentiviral construct delivers hSpCas9 and hygromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-eGFP-Z1
Plasmid#135049PurposeEnhanced Green fluorescent protein expression for mammalian cells under constitutive CMV Promoter in minimal backbone plasmid.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV PromoterAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
EFS-SpdCas9-Dnmt3A/3L-WT
Plasmid#222497PurposeExpresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherryDepositorInsertS. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
UseLentiviralTagsFLAG TagExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterEFSAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE pGK LC3Flux (HaloTag7-mGFP-LC3-STOP)
Plasmid#215015PurposeExpress an autophagy (LC3) reporter in mammalian cells (untested)DepositorInsertMAP1LC3B (Map1lc3b Rat)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHCAG-L2EOP
Plasmid#51783PurposeThe oriP/EBV nuclear antigen (EBNA)-1 elements of Epstein-Barr virus is fused in frame with the luciferase in the herpes simplex virus type-1 amplicon vector, provides prolonged transgene expressionDepositorInsertsLuciferase-2A-EBNA-1
OriP
UseHerpes simplex virus type-1 amplicon vectorTagsExpressionMutationPromoterCAGAvailable sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmIL-6promoterEGFP
Plasmid#112896PurposeTo monitor the activation of the murine IL-6 promoter by eGFP expressionDepositorInsertmurine IL-6 promoter (Il6 Mouse)
UseTagsExpressionMammalianMutationPromoterIL-6 promoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB6K-mcherry-iATPsnFR1.1
Plasmid#227239PurposeInducible ATP sensor for Pseudomonas putidaDepositorInsertiATPsnFR1.1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-Talin rod-mCherry
Plasmid#133567PurposeExpresses SpyTag003-Talin rod-mCherry in mammalian cellsDepositorInsertSpyTag003-Talin rod-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF2841
Plasmid#143380PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertIKZF2 (IKZF2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 c-SRC (WT)
Plasmid#42202DepositorInsertc-SRC (SRC Human)
UseTagsExpressionMammalianMutationL176F mutation compared to reference sequence NM_…PromoterCMVAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) Flag-His-ATM kd
Plasmid#31986DepositorInsertATM kd (ATM Human)
UseTagsFlag and HisExpressionMammalianMutation3'UTRf 9 nucl/D2870A N2875k. catalytically …PromoterAvailable sinceMarch 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE-zCas9
Plasmid#101739Purposeexpression of an optimized Cas9 for base-editing in zebrafishDepositorInsertrAPOBEC1-XTEN-zCas9-UGI-NLS
UseCRISPRTagsExpressionMammalianMutationD10APromoterCMVAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
DNA-PKcs ABCDE>A
Plasmid#83318Purposehuman DNA-PKcs/ABCDE>alaDepositorInserthuman DNA-PKcs (PRKDC Human)
UseTagsExpressionMammalianMutation6 phosphorylation sites in the ABCDE cluster subs…PromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
MGAT2-RpHLuorin2
Plasmid#171718PurposeCis-/medial-Golgi expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 fused to MGAT2 (MGAT2 Human)
UseTagsratiometric pHluorin2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TLR2-YFP
Plasmid#13016DepositorInsertToll-Like Receptor 2 (TLR2 Human)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-2A-MCS2
Plasmid#71782PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
PGAL
Plasmid#60337PurposeContains full length native galactose promoterDepositorInsertspromoter
yECitrine
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
FLX1.8NFATGFPpd2HS4
Plasmid#169096PurposeLentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activationDepositorInsertsNFAT enhancer (from IL-4 promoter)
Destabilized green fluorescent protein
UseLentiviralTagsExpressionMutationPromoterNFAT enhancer + synthetic TATA boxAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
ERK-KTR-mStrawberry reporter
Plasmid#158686PurposeThis plasmid is a ERK pathway reporter. It has CMV promoter driving the expression of ERK-KTR fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only
