165,385 results

1. AAVS1-TRE-U2AF2-TurboID-HA-rtTA

   Plasmid

   #226997

   Purpose

   Integrative plasmid to express U2AF2-TurboID in mammalian cells

   Depositor

   Bruno Dallagiovanna

   Article

   Gomes-Junior et al PLoS One. 2025 May 30;20(5):e0324779. do

   Insert

   U2 small nuclear RNA auxiliary factor 2 (U2AF2 Human)
   

   Tags

   TurboID-HA

   Expression

   Mammalian

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUCmini-iCAP-AAV9.452sub.LUNG1

   Plasmid

   #184592

   Purpose

   non-standard AAV2 rep-AAV9.452sub.LUNG1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system for mouse lung transduction after intravenous injection

   Depositor

   Viviana Gradinaru

   Article

   Goertsen et al Mol Ther Methods Clin Dev. 2022 Aug 1;26

   Insert

   Synthetic construct isolate AAV9.452sub.LUNG1 VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   7 amino acid substitution between VP1 452 and VP1…

   Promoter

   p41

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. iF66 PB-LoxStopLox-Zim3-dCas9-mScarlet-hygro

   Plasmid

   #204731

   Purpose

   Cre-inducible CRISPRi by Zim3-dCas9

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Zim3-dCas9
   

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-SpRYc-ABE8e-EGFP

   Plasmid

   #208340

   Purpose

   Expresses SpRYc-ABE8e in mammalian cells

   Depositor

   Pranam Chatterjee

   Article

   Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

   Insert

   ecTadA(8e)-nSpRYc
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Sc++ (1-1119), SpRY (1111-1368), D10A

   Promoter

   CMV

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)

   Plasmid

   #199605

   Purpose

   Expression of cell permeable opAsCas12a in E. Coli.

   Depositor

   Junwei Shi

   Article

   Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

   Insert

   opCas12a-T8N
   

   Use

   CRISPR

   Tags

   6xHis, Twin-strep, SUMO

   Expression

   Bacterial

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-FLEX-EGFP-mir30(Scn9a-scrambled)

   Plasmid

   #79671

   Purpose

   Scrambled control for Cre-dependent knockdown of Scn9a (Nav1.7)

   Depositor

   Scott Sternson

   Article

   Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

   Insert

   EGFP
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 IKKe flag

   Plasmid

   #26201

   Depositor

   Tom Maniatis

   Article

   Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

   Insert

   I-kappa-B kinase epsilon (IKBKE Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. T-STOP sgRNA-1

   Plasmid

   #83346

   Purpose

   The sgRNA at the stop codon of human Brachyury(T) locus

   Depositor

   James Thomson

   Article

   Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

   Insert

   T-STOP sgRNA
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAM-AAV-hSyn1-EGFP

   Plasmid

   #153205

   Purpose

   Human synapsin-1 (hSyn1) promoter-mediates gene expression in retinal neurons with fluorescent reporter

   Depositor

   Yang Hu

   Article

   Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Available Since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Dendra2-CathespinB-6

    Plasmid

    #57703

    Purpose

    Localization: Lysosomes, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Insert

    CathespinB
    

    Tags

    Dendra2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pJV53

    Plasmid

    #26904

    Depositor

    Graham Hatfull

    Article

    van Kessel et al Nat Methods. 2007 Feb . 4(2):147-52.

    Insert

    Che9c genes 60-61
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-scFv-GCN4-StayGold-IRESv4-HaloTag-tdMCP-CAAX

    Plasmid

    #241144

    Purpose

    Expresses Anti-GCN4 scFv-StayGold, HaloTag-tdMCP, and HaloTag-tdMCP-CAAX to track mature and nascent HA-tagged proteins and track MS2-tagged mRNA anchored to the plasma membrane

    Depositor

    Tim Stasevich

    Article

    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

    Inserts

    Anti-GCN4 scFv
    tdMCP - CAAX
    

    Tags

    (n2)oxStayGold(c4) E147D and HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223 SARS-CoV-2 N

    Plasmid

    #149330

    Purpose

    Gateway-compatible Entry vector, with insert of the nucleocapsid (N) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    N (N SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pDONR221-MFSD6_STOP

    Plasmid

    #161119

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    MFSD6 (MFSD6 Human)
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pAAV-CAG-miRFP720

    Plasmid

    #197213

    Purpose

    Expresses the protein of miRFP720 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    miRFP720
    

    Use

    AAV

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. 4XCLEAR-luciferase reporter

    Plasmid

    #66800

    Purpose

    Reporter for activity of the CLEAR network

    Depositor

    Albert La Spada

    Article

    Cortes et al Nat Neurosci. 2014 Sep;17(9):1180-9. doi

    Insert

    4XCLEAR
    

    Use

    Luciferase

    Promoter

    Four CLEAR elements (GTCACGTGAC) in tandem derive…

    Available Since

    Nov. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. BS-His3

    Plasmid

    #69197

    Purpose

    complementation of yeast auxotrophic marker HIS3

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    HIS3
    

    Expression

    Yeast

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-EF1a-MinD-IRES-Puro

    Plasmid

    #213114

    Purpose

    Expression of untagged MinD in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    MinD (minD Escherichia coli)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRT043-CLYBL-DDdcas9-VPH

    Plasmid

    #158091

    Purpose

    Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

    Depositor

    Martin Kampmann

    Article

    Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

    Insert

    CLYBL-CAG-DDdCas9VPH-T2A-GFP
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. PIK3CA - Exon20 H1047R

    Plasmid

    #16639

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Science. 2004 Apr 23. 304(5670):554.

    Insert

    PIK3CA H1047R (PIK3CA Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    H1047R

    Available Since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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