We narrowed to 8,688 results for: App

1. LCV2_mCherry_AAVS1_sgRNA_4

   Plasmid

   #155106

   Purpose

   lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLCKO_AAVS1_sgRNA_4

   Plasmid

   #155087

   Purpose

   lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LCV2_AAVS1_sgRNA_4

   Plasmid

   #155090

   Purpose

   lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-Syn-FLEX-rc [ArchT-tdTomato]

   Plasmid

   #123607

   Purpose

   AAV mediated expression of ArchT-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. tdTomato has codons varied to reduce recombination. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Han et al Front Syst Neurosci. 2011;5:18. Epub 201

   Insert

   ArchT-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   Syn

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-P70C

   Plasmid

   #114629

   Purpose

   Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 49 polyQ repeat

   Depositor

   Hilal Lashuel

   Article

   Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

   Insert

   Huntingtin Exon1 fragment (HTT Human)
   

   Use

   Tags

   His6-Ssp DnaB intein

   Expression

   Bacterial

   Mutation

   A2C, P70C; 49 polyQ repeat

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTWIN1-His6-Ssp-Httex1-23Q-A2C-P90C

   Plasmid

   #114615

   Purpose

   Bacterial expression of Htt fragment containing exon 1, with A2C & P90C cysteine mutations and a 23 polyQ repeat

   Depositor

   Hilal Lashuel

   Article

   Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

   Insert

   Huntingtin Exon1 (HTT Human)
   

   Use

   Tags

   His6-Ssp DnaB intein

   Expression

   Bacterial

   Mutation

   A2C, P90C; 23 polyQ repeat

   Promoter

   Available since

   Oct. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-A60C

   Plasmid

   #114628

   Purpose

   Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 49 polyQ repeat

   Depositor

   Hilal Lashuel

   Article

   Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

   Insert

   Huntingtin Exon1 fragment (HTT Human)
   

   Use

   Tags

   His6-Ssp DnaB intein

   Expression

   Bacterial

   Mutation

   A2C, A60C; 49 polyQ repeat

   Promoter

   Available since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTWIN1-His6-Ssp-Htt2-90-37Q-P90C

   Plasmid

   #114621

   Purpose

   Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 37 polyQ repeat

   Depositor

   Hilal Lashuel

   Article

   Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

   Insert

   Huntingtin Exon1 fragment (HTT Human)
   

   Use

   Tags

   His6-Ssp DnaB intein

   Expression

   Bacterial

   Mutation

   P90C; 37 polyQ repeat

   Promoter

   Available since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTWIN1-His6-Ssp-Htt2-90(A2C)-23Q-P80C

   Plasmid

   #114614

   Purpose

   Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 23 polyQ repeat

   Depositor

   Hilal Lashuel

   Article

   Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

   Insert

   Huntingtin Exon1 fragment (HTT Human)
   

   Use

   Tags

   His6-Ssp DnaB intein

   Expression

   Bacterial

   Mutation

   A2C, P80C; 23 polyQ repeat

   Promoter

   Available since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTWIN1-His6-Ssp-Htt2-90(A2C)-43Q-P70C

    Plasmid

    #114624

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 43 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, P70C; 43 polyQ repeat

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTWIN1-His6-Ssp-Httex1-43Q-A2C-P90C

    Plasmid

    #114625

    Purpose

    Bacterial expression of Htt fragment containing exon 1, with A2C & P90C cysteine mutations and a 43 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, P90C; 43 polyQ repeat

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-P90C

    Plasmid

    #114630

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 49 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, P90C; 49 polyQ repeat

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P90C

    Plasmid

    #114610

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 15 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, P90C; 15 polyQ repeat

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P70C

    Plasmid

    #114609

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 15 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, P70C; 15 polyQ repeat

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-P90C

    Plasmid

    #114620

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 37 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, P90C; 37 polyQ repeat

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-P80C

    Plasmid

    #114619

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P80C cysteine mutations and a 37 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, P80C; 37 polyQ repeat

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-A60C

    Plasmid

    #114618

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 37 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, A60C; 37 polyQ repeat

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-A60C

    Plasmid

    #114608

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 15 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, A60C; 15 polyQ repeat

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTWIN1-His6-Ssp-Htt2-90(A2C)-23Q

    Plasmid

    #114612

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 23 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C; 23 polyQ repeat

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-dCas9-p300-P2A-PuroR

    Plasmid

    #83889

    Purpose

    Lentiviral Sp dCas9-p300-P2A-PuroR

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    pac from Streptomyces alboniger
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EFS

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge