We narrowed to 9,829 results for: pho;

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Sema3a(L, del3)-AP-His

Plasmid

#72010

Purpose

Expresses the Sema3A protein (truncated at cleavage site P3; ie, long and missing exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3a (Sema3a Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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SFG.CNa22_opt.IRES.eGFP

Plasmid

#22491

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

Insert

Codon optimised Calcineurin A mutant 22 (PPP3CA Human)

Use

Retroviral

Mutation

V314R; Y341F

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pLC-Flag-UBE2G1 sg2R-P2A-Hygro

Plasmid

#124299

Purpose

Lentiviral vector for expression of Flag tagged UBE2G1-P2A-Hygro casette from a CMV promoter. UBE2G1 cDNA contains silent mutations to disrupt a sgRNA binding site.

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

UBE2G1 (UBE2G1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

several silent mutations to disrupt sgRNA targeti…

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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mCerulean3-EB3-C-20

Plasmid

#55417

Purpose

Localization: MT End Binding Protein, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

EB3 (MAPRE3 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pBabe puro H-Ras 12V (40C)

Plasmid

#12590

Depositor

Channing Der

Article

McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

Insert

HRas 12V 40C (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

12V, 40C

Available Since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only
MSCV-Fbxo7(ΔF-box)-IRES-GFP

Plasmid

#171787

Purpose

retrovirus production

Depositor

Heike Laman

Article

Lomonosov et al PLoS One. 2011;6(6):e21165. doi: 10.1371

Insert

Fbxo7 isoform 1 (FBXO7 Human)

Use

Retroviral

Mutation

deletion of aa335-367

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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823 pGEX2T PTEN G129R

Plasmid

#10749

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Tags

GST

Expression

Bacterial

Mutation

G129R

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pT2EFCRET(#324)

Plasmid

#184059

Purpose

cyclofen-inducible CRE activation in zebrafish transient transgenic

Depositor

Sophie Vriz

Article

Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1

Insert

CRE-ERT2

Use

Zebrafish transient transgenesis

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Xl-EF1a

Available Since

July 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1a_ TET1 CXXC only

Plasmid

#124084

Purpose

Expression of CXXC domain only form of TET1

Depositor

Silvia Monticelli

Article

Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

Insert

TET1 - CXXC domain (TET1 Human)

Tags

Flag and HA

Expression

Mammalian

Promoter

EF1 alpha

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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p13xLexAOP2-IVS-Syn21-Voltron-p10

Plasmid

#119042

Purpose

LexAop2-driven expression of Voltron in Drosophila

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron

Expression

Insect

Promoter

hsp70

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe puro H-Ras 12V (35S)

Plasmid

#12588

Depositor

Channing Der

Article

McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

Insert

HRas 12V 35S (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

12V, 35S

Available Since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only
Sema3a(S)-AP-His

Plasmid

#72012

Purpose

Expresses the Sema3A protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3a (Sema3a Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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821 pGEX2T PTEN 1-274

Plasmid

#10741

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Tags

GST

Expression

Bacterial

Mutation

Amino acids 1-274.

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
Sema3b(L)-AP-His

Plasmid

#72013

Purpose

Expresses the Sema3B protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3b (Sema3b Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-HDAC4 C-terminus

Plasmid

#71400

Purpose

CAG promoter, mammalian expression

Depositor

Bo Chen

Article

Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

Insert

HDAC4 C-terminus (HDAC4 Human)

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 29, 2015

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1L16A/L17A

Plasmid

#13478

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1L16A/L17A (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Changed Leucine 16 to Alanine; changed Leucine 1…

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pPB-MPP8-1–729_Flag2-Blast

Plasmid

#175040

Purpose

Mammalian expression of mMPP8 (M1 to E729) with C-terminal Flag2 tag

Depositor

Kristian Helin

Article

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

Mphosph8 (Mphosph8 Mouse)

Use

Piggybac

Tags

Flag2

Expression

Mammalian

Mutation

Expression of MPP8 protein from amino acid positi…

Promoter

CAG

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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814 pSG5L HA PTEN 1-336

Plasmid

#10742

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

Amino acids 1-336.

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pSpCas9_BB_2A-GFP_MAPRE1-gRNA#3

Plasmid

#107728

Purpose

Knock out of EB1 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 gRNA #3 (targets Exon 1) (MAPRE1 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSBbi-Pur-cpVenus[E172]

Plasmid

#125202

Purpose

SB-transposon plasmid for stable expression of cpVenus[E172] cDNA as a FRET single color control

Depositor

Eric Davis

Article

Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

Insert

cpVenus[E172]

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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