We narrowed to 13,684 results for: Egf

1. 2xMyc-tdMCP-SNAP (tdMCP standard)

   Plasmid

   #226166

   Purpose

   For transient expression of the tdMCP-protein standard. Used in LABEL-seq abundance assays.

   Depositor

   Dustin Maly

   Article

   Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

   Insert

   2xMyc-tdMCP-SNAPtag
   

   Use

   Lentiviral

   Tags

   2xMyc-tdMCP and EGFP-IRES-BlastR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmTurquoise2-C1

   Plasmid

   #60560

   Purpose

   Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. N-WASP(CRIB)-mScarlet-I-N-WASP(CRIB)

   Plasmid

   #191451

   Purpose

   Visualizing endogenous & active Cdc42

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

   Insert

   N-WASP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. RNA-Protein Landing Pad

   Plasmid

   #226162

   Purpose

   Used to generate a landing pad in Flp-In cells containing promoters for protein and MS2-circRNA barcode expression

   Depositor

   Dustin Maly

   Article

   Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

   Inserts

   mTagBFP2-P2A-iCasp9
   Reverse tetracycline transactivator 3
   Hygromycin-IRES-EGFP
   

   Use

   Synthetic Biology

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. LifeAct-GFP-cytoB5RR

   Plasmid

   #182577

   Purpose

   Expresses LifeAct-GFP targeted to the ER via the CytB5RR tail anchor sequence

   Depositor

   Uri Manor

   Article

   Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

   Insert

   LifeAct-GFP-cytoB5RR
   

   Use

   Tags

   LifeAct and cytoB5RR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmTurquoise2-N1

   Plasmid

   #60561

   Purpose

   Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pmyc-GFP-TNRC6A-(925-1709)

   Plasmid

   #42004

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   contain aa925-1709

   Promoter

   CMV

   Available since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pmyc-GFP-TNRC6A-(1-924)

   Plasmid

   #42003

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   contain aa1-924

   Promoter

   CMV

   Available since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMA2640

   Plasmid

   #25434

   Purpose

   Retroviral Tet-On vector for CMV-driven rtTA with EGFP and Blasticidin selection

   Depositor

   Mikhail Alexeyev

   Article

   Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

   Insert

   reverse tetracycline-regulated transactivator Advanced
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Dimer cleaved

    Plasmid

    #198199

    Purpose

    FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavage

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    mVenus-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A206K both

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmyc-GFP-TNRC6A-(457-924)

    Plasmid

    #42006

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa457-924

    Promoter

    CMV

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pmyc-GFP-TNRC6A-(1-456)

    Plasmid

    #42005

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa1-456

    Promoter

    CMV

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSP64-GFP-ePDZ-CAAX

    Plasmid

    #135603

    Purpose

    Expression of GFP-ePDZ-CAAX mRNA for microinjection to be used for optogenetics in S. purpuratus

    Depositor

    Mamiko Yajima

    Article

    Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

    Insert

    Lifeact-GFP-ePDZ-CAAX
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    CAAX, EGFP, and ePDZ

    Expression

    Mutation

    Promoter

    Unknown

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmGFP-P2A-K(AAG)20-P2A-RFP

    Plasmid

    #105689

    Purpose

    Double fluorescent stalling reporter K(AAG)20

    Depositor

    Ramanujan Hegde

    Article

    Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

    Inserts

    P2A-3xFLAG-VHPbeta
    K(AAG)20
    P2A-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (from vector)

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mCherry-PAK2 binding domain

    Plasmid

    #83951

    Purpose

    FLIM acceptor for mEGFP Rac1

    Depositor

    Ryohei Yasuda

    Article

    Hedrick et al Nature. 2016 Sep 28;538(7623):104-108. d

    Insert

    Pak2 (PAK2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    aa60-113 only; S74A F84A

    Promoter

    Available since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-REPORT (PGK) NanoLuc

    Plasmid

    #208576

    Purpose

    Empty NanoLuc Lentiviral reporter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    NanoLuciferase
    d2eGFP
    Empty response element
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Tags

    3x SV40 NLS and PEST

    Expression

    Mammalian

    Mutation

    Promoter

    PGK and miniP

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHJ421

    Plasmid

    #20680

    Depositor

    Stephen Shaw

    Article

    Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

    Insert

    Ezrin (EZR Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. px458_2A_GFP_sgRNA_TIAL1

    Plasmid

    #106096

    Purpose

    CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIAL1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    gRNA TIAL1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pACT5FVMF(f)

    Plasmid

    #110549

    Purpose

    Control plasmid for titering FV by qPCR to determine the genome-containing particle number. This qPCR protocol is described in Hendrie et al., 2008

    Depositor

    David Russell

    Article

    Hendrie et al Mol Ther. 2008 Mar;16(3):534-40. doi: 10

    Insert

    EGFP, FV LTRs
    

    Use

    Qpcr control for titering foamy virus

    Tags

    Expression

    Mutation

    Promoter

    promoter: MLV LTR

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA5/FRT-ChemoG5

    Plasmid

    #193807

    Purpose

    CMV overexpression of ChemoG5 in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG5
    

    Use

    Tags

    10xHis

    Expression

    Mammalian

    Mutation

    EGFP:A206K, T225R; HT7: E143R, E147R, L271E

    Promoter

    CMV

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GFP-rab9 WT

    Plasmid

    #12663

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB9 (Homo sapiens) (RAB9A Human)
    

    Use

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pmCherry-C1-p63RhoGEF

    Plasmid

    #67896

    Purpose

    Expresses a fusion of mCherry with full-length p63RhoGEF in mammalian cells

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    mCherry-p63RhoGEF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. eTC GFP beta-actin full length

    Plasmid

    #27123

    Depositor

    Robert Singer

    Article

    Rodriguez et al J Cell Biol. 2006 Oct 9. 175(1):67-76.

    Insert

    beta-actin (ACTB Human)
    

    Use

    Tags

    EGFP and TC tag

    Expression

    Mammalian

    Mutation

    E237A

    Promoter

    Available since

    Jan. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pPB-CAG-Brainbow3.2-PGK-PuroTK

    Plasmid

    #159928

    Purpose

    Brainbow transgenesis

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Res. 2021 Jan 8;51:102164. doi

    Inserts

    NFPnls
    mOrange2
    EGFP
    mKate2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. NLS-SuperNova

    Plasmid

    #131858

    Purpose

    Expresses SuperNova fused to nuclear localization signal (NLS)

    Depositor

    Geert van den Bogaart

    Article

    Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

    Insert

    SuperNova
    

    Use

    Tags

    Myc tag and c-myc nuclear localization signal (NL…

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pmGFP-P2A-K(AAA)12-P2A-RFP

    Plasmid

    #105687

    Purpose

    Double fluorescent stalling reporter K(AAA)12

    Depositor

    Ramanujan Hegde

    Article

    Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

    Inserts

    P2A-3xFLAG-VHPbeta
    K(AAA)12
    P2A-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (from vector)

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAG-hCas9

    Plasmid

    #51142

    Purpose

    Expresses hCas9 under the CAG promoter for CRISPR

    Depositor

    Izuho Hatada

    Article

    Horii et al Int J Mol Sci. 2013 Sep 30;14(10):19774-

    Insert

    hCas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

28. 3xnls-G-Ca-FLITS

    Plasmid

    #191463

    Purpose

    Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    G-Ca-FLITS
    

    Use

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. SarI T39N YFP

    Plasmid

    #128155

    Purpose

    Expression of Sar1 with T39N dominant negative mutation

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    Sar1
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    T39N

    Promoter

    Available since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCoofy57

    Plasmid

    #122009

    Purpose

    Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His, PreScission Cleavage site and different C-tag options

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    His8 and Multi1: depending on the LP2 primer, eit…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge