We narrowed to 22,696 results for: Sis;

1. UKNP3.2.1

   Plasmid

   #98222

   Purpose

   Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

   Depositor

   Jonathan Ball

   Article

   Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

   Insert

   HCV E1E2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. SFG.CNa22_opt.IRES.eGFP

   Plasmid

   #22491

   Depositor

   Martin Pule

   Article

   Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

   Insert

   Codon optimised Calcineurin A mutant 22 (PPP3CA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   V314R; Y341F

   Promoter

   Available Since

   Dec. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEPQD1CB0108

   Plasmid

   #177041

   Purpose

   MoClo Level 1, position 2, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies driven by 35S promoter

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   geranyl pyrophosphate synthase (PaGPPS1) from Picea abies
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEPQD1CB0817

   Plasmid

   #177039

   Purpose

   MoClo Level 1, position 1, transcriptional unit for transient expression of truncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase (AstHMGR1) from Avena strigosa driven by 35S promoter

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   truncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase (AstHMGR1) from Avena strigosa
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHH0103_NCK1-2/3

   Plasmid

   #91420

   Purpose

   Protein expression and purification of human SH3 domain construct NCK1-2/3

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   NCK1-2/3 (NCK1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. optoPKAW196R/F327A

   Plasmid

   #104547

   Purpose

   Expresses Cry2-mCherry-PKA W196R/F327A in mammalian cells.

   Depositor

   David Lawrence

   Article

   O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

   Insert

   Cry2-mCherry-PKA W196R/F327A
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   PKA W196R and Y204A

   Promoter

   CMV

   Available Since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKF-D2irTNP2AG-T2APuroR-5kwh

   Plasmid

   #128255

   Purpose

   Mammalian linearizer gene circuit based on negative feedback in a Flp-In expression system controlling the PuroR gene.

   Depositor

   Gabor Balazsi

   Article

   Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

   Insert

   hTetR::P2A::EGFP::T2A::PuroR
   

   Use

   Synthetic Biology; Flp-in expression vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-D2ir promoter

   Available Since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-ERBB4 (R544W)

   Plasmid

   #29531

   Depositor

   Yardena Samuels

   Article

   Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

   Insert

   ERBB4 (ERBB4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed arginine 544 to tryptophan

   Promoter

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

9. UKNP6.1.2

   Plasmid

   #98388

   Purpose

   Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

   Depositor

   Jonathan Ball

   Article

   Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

   Insert

   HCV E1E2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-kanMX6-3nmt1-mMaple3

    Plasmid

    #105167

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Liu et al Mol Biol Cell. 2016 Aug 15;27(16):2528-4

    Insert

    kanMX6-3nmt1-mMaple3
    

    Use

    Tags

    3nmt1-mMaple3

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    3nmt1

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_UBASH3A-1/1

    Plasmid

    #91478

    Purpose

    Protein expression and purification of human SH3 domain construct UBASH3A-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    UBASH3A-1/1 (UBASH3A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. SIN40C.SFFV.dTomato.miR-ctrl

    Plasmid

    #169303

    Purpose

    Constitutive overexpression of miR-ctrl with dTomato as a reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    miR-ctrl
    

    Use

    Lentiviral

    Tags

    dTomato

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. UKNP2.2.1

    Plasmid

    #98209

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. miR-144 Trap

    Plasmid

    #60934

    Purpose

    to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of complementary miR-144-binding sites cloned 3' to GFP under the control of a biPGK promoter

    Depositor

    Curt Civin

    Article

    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

    Insert

    miR-144 Trap
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    bidirectional phosphoglycerate kinase promoter (b…

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pBGC155-MXMT

    Plasmid

    #79818

    Purpose

    GFP(S65T)-based BiFC vector for plants (Positive control: MXMT)

    Depositor

    Masamitsu Wada, Yutaka Kodama

    Article

    Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

    Insert

    CaMXMT1
    

    Use

    Reporter plasmid

    Tags

    GC155/GFP (155–239)

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter and CaMV35S

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPK1E-1+t

    Plasmid

    #48147

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-h-mmPtch1-B

    Plasmid

    #120888

    Purpose

    Expresses Ptch1-B for function assays

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    Ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXIP TGBRI K to R

    Plasmid

    #19150

    Depositor

    Joan Massague

    Article

    Padua et al Cell. 2008 Apr 4. 133(1):66-77.

    Insert

    Transforming growth factor, beta receptor I (TGFBR2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K420R mutation

    Promoter

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pCRISPR01-FnCpf1

    Plasmid

    #176529

    Purpose

    Nuclease Cpf1 adapted for Aspergillus species

    Depositor

    Kusol Pootanakit

    Article

    Abdulrachman et al BMC Biotechnol. 2021 Feb 11;21(1):15. do

    Insert

    FnCpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    Expression

    Mutation

    Promoter

    TEF1

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. 2Bc-T MBP_BoMoC(ed)_I770A_6xH

    Plasmid

    #185711

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

    Insert

    B. mori truncated R2 RT
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Changed I770 to A

    Promoter

    T7

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCEP4-SERTS585P-myc

    Plasmid

    #107498

    Purpose

    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S585P conferring gain of function phenotype

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S585P

    Promoter

    CMV

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAC-mWasabi-G4

    Plasmid

    #164168

    Purpose

    Expresses mWasabi under the control of CMV promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mWasabi
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCarCBA

    Plasmid

    #117754

    Purpose

    Codon optimized CarCBA enzymes from Pectobacterium carotovorum in pBbaA5k backbone

    Depositor

    Greg Bokinsky

    Article

    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

    Insert

    Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLac(UV5)

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-M2-TLR2-V660N

    Plasmid

    #12292

    Depositor

    Bruce Beutler

    Article

    Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    TLR2 (Tlr2 Mouse)
    

    Use

    Tags

    FLAG (FLAG-M2)

    Expression

    Mammalian

    Mutation

    M82I, H202Y and V660N; signal peptide (aa1-23) re…

    Promoter

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

25. SaABE8e-dimer

    Plasmid

    #138501

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-nSaCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p5B3-T

    Plasmid

    #92021

    Purpose

    Expresses TALE targeted to lysA promoter with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lysA with N-terminal TEV protease cleavage site
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Expression

    Mutation

    S219V

    Promoter

    Available Since

    June 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGWB427

    Plasmid

    #74821

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with T7 (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGWB426

    Plasmid

    #74820

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with T7 (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Flag-ATXN1_L588D

    Plasmid

    #48194

    Purpose

    Expresses L588D mutated Ataxin1

    Depositor

    Huda Zoghbi

    Article

    Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

    Insert

    Ataxin1 (ATXN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Flag-ATXN1_L588A

    Plasmid

    #48193

    Purpose

    Expresses L588A mutated Ataxin1

    Depositor

    Huda Zoghbi

    Article

    Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

    Insert

    Ataxin1 (ATXN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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