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We narrowed to 2,572 results for: GEM

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Showing: 1441 - 1480 of 2572 results

  4. pCEP4-HA-Flag-natT1R2 RR837-8GG

    Plasmid
    #113958
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    residues 22-839, R837G and R838G substitution
    Promoter
    cmv
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113958-plasmid-map-sequence-id-215287

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  13. p2R3a-GUS-OcsT

    Plasmid
    #71267
    Purpose
    Entry clone containing the GUS enzyme. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Article
    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246
    Insert
    Beta-glucuronidase
    Use
    Gateway
    Tags
    2xGly linker and octaline synthase terminator
    Available Since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    71267-plasmid-map-sequence-id-129805

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  14. MultiMate-Rainbow

    Plasmid
    #206252
    Purpose
    Expression of 7 fluorescently labelled proteins in mammalian cells. Can be used to generate recombinant baculovirus particles.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    H2B (human); Actin, Tubulin (B. taurus); mito mCherry, GST mTagBFP1 (Synthetic); CyOFP1 (E. quadricolor); Ctnnb1 (mouse)
    Use
    Recombinant baculovirus production (bac-to-bac)
    Tags
    iRFP713
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206252-plasmid-map-sequence-id-406651

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  16. pcDNA3.1(+)-HLA-cmyc-optiT1R3 a21-852

    Plasmid
    #113949
    Purpose
    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R3 (TAS1R3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    residues 21-852
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113949-plasmid-map-sequence-id-224194

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  19. pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry

    Plasmid
    #35498
    Purpose
    AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.
    Depositor
    Karl Deisseroth
    Article
    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi
    Insert
    ChR1-VChR1 Chimera
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    E122T and E162T
    Promoter
    Ef1a
    Available Since
    April 18, 2012
    Availability
    Academic Institutions and Nonprofits only
    35498-plasmid-map-sequence-id-199355

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  20. pcDNA3-HLA-cMyc-EcopT1R1

    Plasmid
    #113962
    Purpose
    mammalian expression plasmid for c-myc-tagged E. coli codon-optimized human T1R1 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R1 (TAS1R1 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    truncate N-terminal 24 residues
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113962-plasmid-map-sequence-id-223994

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  21. pAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP

    Plasmid
    #35499
    Purpose
    AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.
    Depositor
    Karl Deisseroth
    Article
    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi
    Has Service
    AAV9
    Insert
    ChR1-VChR1 Chimera
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    E122T and E162T
    Promoter
    CaMKIIa
    Available Since
    April 18, 2012
    Availability
    Academic Institutions and Nonprofits only
    35499-plasmid-map-sequence-id-199323

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  22. pAAV-Ef1a-DIO C1V1 (t/t)-TS-EYFP

    Plasmid
    #35497
    Purpose
    AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.
    Depositor
    Karl Deisseroth
    Article
    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi
    Has Service
    AAV9
    Insert
    ChR1-VChR1 Chimera
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    E122T and E162T
    Promoter
    Ef1a
    Available Since
    April 18, 2012
    Availability
    Academic Institutions and Nonprofits only
    35497-plasmid-map-sequence-id-196845

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  28. pFrt-invCAG-ires-Luc

    Plasmid
    #63576
    Purpose
    Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional cDNA and Luc expression upon integration. The vector contains an FRT site and two mut loxP sites.
    Depositor
    Ivo Huijbers
    Article
    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi
    Insert
    Stop-Lox71-IRES-Luciferase-pA-FRT-Lox66-reverseCAG
    Use
    Cre/Lox; Frt/flpe recombination mediated insertion
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    63576-plasmid-map-sequence-id-105724

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  29. pCEP4-HA-Flag-natT1R2 ECD-MHC

    Plasmid
    #113957
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 ECD with signal peptide of influenza hemagglutinin fused to a canonical transmembrane domain from MHC class I
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Tags
    FLAG, Signal/leader sequence from influenza hemag…
    Expression
    Mammalian
    Mutation
    residues 22-568 fused to a transmembrane tether
    Promoter
    cmv
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113957-plasmid-map-sequence-id-215285

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  30. pCMV_NFL(K363TAG)-FLAG

    Plasmid
    #182656
    Purpose
    Expresses mouse neurofilament light chain with a TAG codon at the position K363 & C-terminal FLAG tag (DYKDDDDK) in mammalian cells. Can be used for amber codon suppression & click labeling of NFL.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse neurofilament light chain with a K363TAG mutation (Nefl Mouse)
    Tags
    FLAG tag (DYKDDDDK)
    Expression
    Mammalian
    Mutation
    K363TAG
    Promoter
    CMV
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182656-plasmid-map-sequence-id-363073

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  31. pCEP4-HLA-c-myc-optiT1R3 ECD

    Plasmid
    #113960
    Purpose
    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R3 (TAS1R3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    residues 21-563
    Promoter
    cmv
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113960-plasmid-map-sequence-id-215290

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  33. pAAV hSyn1 CaRhGC wt T2A tDimer

    Plasmid
    #101720
    Purpose
    humanized, Neuron-specific promoter driven expression of the rhodopsin guanylyl cyclase from Catenaria anguillulae with a neuron-specific promoter. Useful for raising intracellular cAMP close to the m
    Depositor
    Thomas Oertner
    Article
    Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:
    Inserts
    CatRhGC
    red fluorescent protein
    Use
    AAV
    Expression
    Mammalian
    Promoter
    hSyn1 and ribosomal skip sequence T2A
    Available Since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    101720-plasmid-map-sequence-id-201303

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  35. pCol1a1-frt (FVB)

    Plasmid
    #63575
    Purpose
    Targeting vector for the Col1a1 locus that contains a Neo resistance cassette flanked by FRT sites, followed by an ATG-less Hygromycine resistance gene. The homology arms match the sequence of FVB.
    Depositor
    Ivo Huijbers
    Article
    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi
    Insert
    Col1A-frt-hygro-pA
    Use
    Mouse Targeting; Frt/flpe
    Expression
    Bacterial and Mammalian
    Promoter
    None
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63575-plasmid-map-sequence-id-105719

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  40. pMMK ENTR 3 CMV mito mCherry

    Plasmid
    #206256
    Purpose
    ENTR Vector 3 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mitochondrially localised mCherry under the control of CMV promoter.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    mito mCherry
    Use
    Multimate/gateway entr 3
    Tags
    COX8 mitochondrial tag
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206256-plasmid-map-sequence-id-406392

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Showing: 1441 - 1480 of 2572 results