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Showing: 1801 - 1850 of 2174 results

  5. p2B-58

    Plasmid
    #177928
    Purpose
    Expresses yeGFP under control of doxycycline-responsive TET4 promoter (contains four rtTA binding sites)
    Depositor
    Vadim Gladyshev
    Article
    Barre et al (unpublished)
    Insert
    EGFP
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    TET4 (contains four rtTA binding sites)
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177928-plasmid-map-sequence-id-349996

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  6. p2B-57

    Plasmid
    #177927
    Purpose
    Expresses yeGFP under control of doxycycline-responsive TET3 promoter (contains three rtTA binding sites)
    Depositor
    Vadim Gladyshev
    Article
    Barre et al (unpublished)
    Insert
    EGFP
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    TET3 (contains three rtTA binding sites)
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177927-plasmid-map-sequence-id-349993

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  7. pLVTHM

    Plasmid
    #12247
    Purpose
    2nd gen lentivector expressing shRNA from H1 promoter and GFP. For direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from pSUPER. Dox-controllable if used with tTRKRAB expressing plasmid
    Depositor
    Didier Trono
    Article
    Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12247-plasmid-map-sequence-id-352425

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  8. pAX2

    Plasmid
    #117398
    Purpose
    allelic exchange vector with GFP merodiploid tracker, temperature-sensitive origin of replication, and TetR-controlled kill switch
    Depositor
    Karen Guillemin
    Article
    Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    117398-plasmid-map-sequence-id-230535

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  11. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

    Plasmid
    #217635
    Purpose
    AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.
    Depositor
    Martin Kampmann
    Insert
    mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    EF1alpha and mU6
    Available since
    Oct. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    217635-plasmid-map-sequence-id-449624

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  12. pDECKO_mCherry_AAVS1

    Plasmid
    #141212
    Purpose
    pDECKO_mCherry_AAVS1_186. Targeting, non-essential locus control
    Depositor
    Rory Johnson
    Insert
    gRNA
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    141212-plasmid-map-sequence-id-299605

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  17. AAV-∆Hapln1

    Plasmid
    #200778
    Purpose
    Expresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoter
    Depositor
    Alexander Dityatev
    Article
    Ramsaran et al Science. 2023 May 5;380(6644):543-551. d
    Insert
    Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
    Use
    AAV
    Tags
    cfGFP
    Expression
    Mutation
    deleted lectican binding domain (aminoacids 40-15…
    Promoter
    Synapsin
    Available since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    200778-plasmid-map-sequence-id-394043

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  19. pLY70

    Plasmid
    #130948
    Purpose
    A CRISPR activation device with the necessary genes (dxcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter PrhaB), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dxcas9 (3.7)
    tetR
    rhaS
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9 for dCas9; Sy…
    Promoter
    Pcon, PpspA-LEA3B3, PrhaB, and Ptet
    Available since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    130948-plasmid-map-sequence-id-257361

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  20. pLY61

    Plasmid
    #130928
    Purpose
    A CRISPR activation device with the necessary genes (dxcas9 3.7 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dxcas9 (3.7)
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9 for dCas9; Sy…
    Promoter
    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet
    Available since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    130928-plasmid-map-sequence-id-257360

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  23. MCP-MSN

    Plasmid
    #210704
    Purpose
    This plasmid express EF1a promoter driven MCP-MSN transactivation module
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210704-plasmid-map-sequence-id-416247

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  24. MCP-eN3x9

    Plasmid
    #210706
    Purpose
    This plasmid express EF1a promoter driven MCP-eN3x9 transactivation module
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to transactivation domain comprising eNRF2 and 3x 9aa Transactivation domain
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210706-plasmid-map-sequence-id-416266

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  25. MCP-NMS

    Plasmid
    #210705
    Purpose
    This plasmid express EF1a promoter driven MCP-NMS transactivation module
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to transactivation domain from Human transcription factor NRF2, MRTF-A and STAT1
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210705-plasmid-map-sequence-id-416249

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  28. pCIBN-tagBFP-hTRF2

    Plasmid
    #103803
    Purpose
    BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-tagBFP-hTRF2 (CIB1, TERF2 Human, Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hTRF2: deletion of amino acids 1-42 and 476 compa…
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103803-plasmid-map-sequence-id-199226

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  30. MCP-3x9aaTAD

    Plasmid
    #210707
    Purpose
    This Plasmid express EF1a promoter driven MCP-3x 9aa transactivation module
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to 3x 9aa transactivation domain derived from MRTF-B and MYOCD1
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210707-plasmid-map-sequence-id-416252

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  31. pCIBN-EYFP-hTRF2

    Plasmid
    #103804
    Purpose
    BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-EYFP-hTRF2 (CIB1, TERF2 Human, Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hTRF2: deletion of amino acids 1-42 and 476 compa…
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103804-plasmid-map-sequence-id-199232

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  34. pCIBN-TetR-tagRFP-T

    Plasmid
    #103809
    Purpose
    'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-TetR-tagRFP-T (CIB1, tetR Mustard Weed, E. coli)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    TetR: A4G (M2V)
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103809-plasmid-map-sequence-id-199261

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  35. pTon1(il11)

    Plasmid
    #233647
    Purpose
    Expression vector of X. laevis il11.L-P2A-AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.
    Depositor
    Takeo Kubo
    Article
    Tsujioka et al Nat Commun. 2017 Sep 8;8(1):495. doi: 10
    Inserts
    interleukin-11.L (350-913) (il11.L Frog)
    target sequence of guide RNA #tyr (3905-3927) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
    Use
    Amphibian expression, tet-on
    Tags
    Expression
    Mutation
    Some synonymous substitutions were introduced in…
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233647-plasmid-map-sequence-id-468199

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  37. pEB1N-LZ-mCherry-LOV2(wt)

    Plasmid
    #107693
    Purpose
    N-terminal half of π-EB1 with wild-type LOV2, mCherry-tagged to track microtubule ends
    Depositor
    Torsten Wittmann
    Article
    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038
    Insert
    MAPRE1 (MAPRE1 Human)
    Use
    Tags
    A. sativa phototropin 1 LOV2 domain, GCN4 leucine…
    Expression
    Mammalian
    Mutation
    EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…
    Promoter
    CMV
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    107693-plasmid-map-sequence-id-209378

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  41. pCIBN-hTRF2-tagRFP-T

    Plasmid
    #103812
    Purpose
    BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-hTRF2-tagRFP-T (CIB1, TERF2 Human, Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hTRF2: deletion of amino acids 1-42 and 476 compa…
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103812-plasmid-map-sequence-id-199277

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  42. pCIBN-tagBFP-hNCL

    Plasmid
    #103805
    Purpose
    BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-tagBFP-hNCL (CIB1, NCL Human, Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103805-plasmid-map-sequence-id-199237

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  43. pCIBN-LacI

    Plasmid
    #103814
    Purpose
    BLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-LacI (CIB1, lacI Mustard Weed, E. coli)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    LacI: C19T (silent, L7L), T264C (silent, A88A), C…
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103814-plasmid-map-sequence-id-199324

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  44. pCIBN-EYFP-hNCL

    Plasmid
    #103806
    Purpose
    BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-EYFP-hNCL (CIB1, NCL Human, Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103806-plasmid-map-sequence-id-199240

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  48. pRYG

    Plasmid
    #113642
    Purpose
    Positive control: shows the ratio of GFP signal to RFP signal with a tyrosine residue in the linker that connects the two genes.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    mRFP1-linker-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    113642-plasmid-map-sequence-id-214560

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  50. DT037A

    Plasmid
    #159398
    Purpose
    Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23101 (strong) promoter
    Depositor
    Sheref Mansy
    Article
    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea
    Insert
    pr.J23101-sfGFP
    Use
    Synthetic Biology
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    J23101 (strong) promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    159398-plasmid-map-sequence-id-324298

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Showing: 1801 - 1850 of 2174 results