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Showing: 1801 - 1850 of 17296 results

  5. beta catenin binding mutant of alpha catenin sensor

    Plasmid
    #71711
    Purpose
    This form of the alpha catenin sensor lacks the beta catenin binding site and does not localize to cadherin junctions
    Depositor
    Deborah Leckband
    Article
    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi
    Insert
    alpha-catenin Δβ-cat mutant FRET Sensor
    Tags
    ECFP and YPET
    Expression
    Mammalian
    Mutation
    Deleted amino acid residues 48- 163
    Promoter
    CMV
    Available Since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71711-plasmid-map-sequence-id-142367

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  6. VBS mutant of alpha catenin sensor

    Plasmid
    #71710
    Purpose
    This form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.
    Depositor
    Deborah Leckband
    Article
    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi
    Insert
    alpha-catenin ΔVBS FRET Sensor
    Tags
    ECFP and YPET
    Expression
    Mammalian
    Mutation
    Deleted amino acid residues 316-405 in α -catenin…
    Promoter
    CMV
    Available Since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71710-plasmid-map-sequence-id-142366

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  17. pJTB115

    Plasmid
    #248909
    Purpose
    Expresses yeast Smk1 and Ssp2 fused to glutathione-S-transferase in bacteria
    Depositor
    Edward Winter
    Article
    Tio et al Mol Cell Biol. 2017 May 2;37(10):e00607-
    Inserts
    SMK1 Mitogen-activated Protein Kinase (SMK1 Budding Yeast, Sequence is from the SK1 isolate of S. cerevisiae and will differ by 1 non-synonomous/several synonymous changes)
    Ssp2 (SSP2 Budding Yeast, Sequence is from the SK1 isolate of S. cerevisiae and may differ by synonymous changes from S288C form)
    Tags
    glutathione-S-transferase
    Expression
    Bacterial
    Mutation
    NdeI and NcoI sites were removed for cloning purp…
    Promoter
    T7
    Available Since
    Jan. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    248909-plasmid-map-sequence-id-497710

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  22. pJH063

    Plasmid
    #243723
    Purpose
    Expresses a chimeric form of SUPV3L1, where the region between residue 114 and 351 is replaced by a 250-residue XTEN linker
    Depositor
    Jonathan Weissman
    Article
    Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.
    Insert
    SUPV3L1(1-114aa)-XTEN250-SUPV3L1(351aa-stop) (SUPV3L1 Human)
    Use
    Lentiviral
    Mutation
    The region between residue 114 and 351 of SUPV3L1…
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    243723-plasmid-map-sequence-id-488887

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  23. pJH106

    Plasmid
    #243727
    Purpose
    Expresses a chimeric form of PMPCA, where the region between residue 100 and 351 is replaced by a 250-residue XTEN linker
    Depositor
    Jonathan Weissman
    Article
    Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.
    Insert
    PMPCA(1-100aa)-XTEN250-PMPCA(351aa-stop) (PMPCA Human)
    Use
    Lentiviral
    Mutation
    The region between residue 100 and 351 of PMPCA i…
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    243727-plasmid-map-sequence-id-488884

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  24. pLV-SFFV-CKAP4(1-131)-WPRE-UbC-Emerald

    Plasmid
    #225955
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutant lacking the luminal domain under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Truncated CKAP4 (1-131) lacking the luminal domain
    Promoter
    SFFV and UbC
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225955-plasmid-map-sequence-id-446861

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  25. pLV-SFFV-RTN4-WPRE-UbC-Emerald

    Plasmid
    #225938
    Purpose
    Lentiviral vector plasmid expressing human reticulon 4 (RTN4) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    RTN4 (RTN4 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV and UbC
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225938-plasmid-map-sequence-id-446791

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  26. pcs2-cDELE1(142-515)-sumo

    Plasmid
    #226107
    Purpose
    cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) fused to sumo used for in-vitro translation by wheat germ extract in in-vitro ubiquitylation assays
    Depositor
    Michael Rape
    Article
    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:
    Insert
    DELE1 (DELE1 Human)
    Tags
    Sumo
    Expression
    Mammalian
    Mutation
    encodes only residues 142-515 of DELE1 (represent…
    Available Since
    Oct. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    226107-plasmid-map-sequence-id-449375

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  27. pCAG_SRGAP2A_GAP(R527L_K566A)

    Plasmid
    #222617
    Purpose
    Expresses full length SRGAP2 with R527L, K566A mt in the Rac1-specific GAP domain that abrogates the GTPase activating function of SRGAP2 without locking the GAP-domain in a form bound to Rac1-GDP.
    Depositor
    Franck Polleux
    Article
    Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1
    Insert
    SRGAP2 (SRGAP2 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Point mutations R527L and K566A (Rac1-specific GA…
    Available Since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    222617-plasmid-map-sequence-id-448408

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  32. T40A+Q54A

    Plasmid
    #217913
    Purpose
    A plasmid encoding the hydrogel-forming protein PXP with both the T40A and Q54A coil mutations
    Depositor
    Cole DeForest
    Article
    Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d
    Insert
    PXP T40A+Q54A
    Tags
    6x His (N and C term)
    Expression
    Bacterial
    Mutation
    Threonine at position 41 and 253 mutated to Alani…
    Promoter
    T5
    Available Since
    June 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    217913-plasmid-map-sequence-id-437015

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  33. pCMV6-AN-DDK_WT RAD23B_aa 80-409 (delta UBL domain)

    Plasmid
    #201545
    Purpose
    Expresses a mutant form of human RAD23B that lacks the N-terminal UBL domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    UV excision repair protein RAD23 homolog B (RAD23B Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Lacks N-terminal residues 1-79
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201545-plasmid-map-sequence-id-395054

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  34. pCMV6-AN-DDK_WT RAD23B_delta aa 188-228 (delta UBA1 domain)

    Plasmid
    #201546
    Purpose
    Expresses a mutant form of human RAD23B that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    UV excision repair protein RAD23 homolog B (RAD23B Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Lacks residues 188-228
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201546-plasmid-map-sequence-id-395053

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  35. pCMV6-AN-DDK_WT RAD23B_delta aa 276-339 (delta XPC-binding domain)

    Plasmid
    #201547
    Purpose
    Expresses a mutant form of human RAD23B that lacks the XPC-binding domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    UV excision repair protein RAD23 homolog B (RAD23B Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Lacks residues 276-339
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201547-plasmid-map-sequence-id-395051

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  36. pCMV6-AN-DDK_aa 1-363 RAD23B (delta UBA2 domain)

    Plasmid
    #201548
    Purpose
    Expresses a mutant form of human RAD23B that lacks the UBA2 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    UV excision repair protein RAD23 homolog B (RAD23B Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Lacks C-terminal residues 364-409
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201548-plasmid-map-sequence-id-395045

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  37. pHW539

    Plasmid
    #198811
    Purpose
    Dominant negative form of Protein Kinase A (PKA). Lowers PKA activity in targeted neurons.
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::PKA(DN)-SL2-GFP::let-858 3'UTR
    Expression
    Worm
    Mutation
    G310D mutation to abolish the binding of cAMP of …
    Available Since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    198811-plasmid-map-sequence-id-391700

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  38. rd

    Plasmid
    #112912
    Purpose
    Express the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tag
    Depositor
    C. James McKnight
    Article
    Chen et al J Biol Chem. 2013 Mar 22;288(12):8313-20
    Insert
    dematin (DMTN Human)
    Tags
    Glutatione-S-Tranferase and precision protease si…
    Expression
    Bacterial
    Mutation
    PEST sequence near the N-terminal removed. 5′-GCG…
    Promoter
    T7
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112912-plasmid-map-sequence-id-220213

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  48. pMA_Int

    Plasmid
    #110096
    Purpose
    E. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Insert
    mycophage integrase
    Use
    Required for transformation of attb integrating v…
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110096-plasmid-map-sequence-id-211299

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  49. pH3mCHSH2

    Plasmid
    #101053
    Purpose
    This is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.
    Depositor
    Jennifer Lodge
    Article
    Upadhya et al Fungal Genet Biol. 2017 Sep 1. pii: S108
    Insert
    Cryptococcus mCherry expression plasmid
    Expression
    Yeast
    Promoter
    Cryptococcus Histone3 promoter
    Available Since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    101053-plasmid-map-sequence-id-192942

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  50. pGH00.0126

    Plasmid
    #64257
    Purpose
    Binary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformation
    Depositor
    Mark Guiltinan, Siela Maximova
    Article
    Maximova et al Plant Cell Rep. 2003 Jun;21(9):872-83. E
    Inserts
    EGFP
    neomycin phosphotransferase II
    Use
    Binary agrobacterium vector for plant transformat…
    Expression
    Plant
    Promoter
    E12-omega
    Available Since
    June 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    64257-plasmid-map-sequence-id-115529

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Showing: 1801 - 1850 of 17296 results