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Plasmid#101003PurposeConstruct for fusion expression of GeNL calcium reporter and neomycin/G418 resistance gene by a bidirectional promoterDepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsExpressionMutationPromoterAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only
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tFucci(CA)5
Plasmid#153521PurposeNew color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(CA)5.DepositorInserttFucci(CA)5.
UseTagsAzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)ExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-mCherry-cNLS
Plasmid#138527PurposeInducible expression of mCherry with nuclear localization signalDepositorInsertmCherry
UseTags3x SV40 cNLSExpressionMammalianMutationPromoterTRE3GsAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
tFucci(SA)5
Plasmid#153520PurposeNew color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.DepositorInserttFucci(SA)5.
UseTagsAzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)ExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-tdTomato-cNLS
Plasmid#138529PurposeInducible expression of tdTomato with nuclear localization signalDepositorInserttdTomato
UseTags3x SV40 cNLSExpressionMammalianMutationPromoterTRE3GsAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pSEVA221pmCDA1-T7RNAP
Plasmid#167978PurposeExpresses fusion protein pmCDA1-T7RNAP in E. coliDepositorInsertpmCDA1-T7RNAP (T7p07 Synthetic)
UseTagsT7 tag and T7RNAPExpressionBacterialMutationPromoterTetR-PtetAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD_TypeIC_Cascade_cas3_invivo
Plasmid#196400Purposeexpresses CRISPR Cascade-Cas3 Type IC proteins for in vivo assayDepositorInsertsCas5c
Cas7c
Cas8c
Cas3c
UseTags6xHisExpressionBacterialMutationPromoteraraBADAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b PKA Cat
Plasmid#14921DepositorInsertPKA catalytic subunit alpha (Prkaca Mouse)
UseTags6xHis and Thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221rAPOBEC1-T7RNAP
Plasmid#167979PurposeExpresses fusion protein rAPOBEC1-T7RNAP in E. coliDepositorInsertrAPOBEC1-T7RNAP (T7p07 Synthetic)
UseTagsT7 tag and T7RNAPExpressionBacterialMutationPromoterTetR-PtetAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB402SC
Plasmid#224552PurposeEmpty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes C-terminal Twin-Strep-HA tags. Duplicated 35S promoter and 35S terminator.DepositorTypeEmpty backboneUseTagsTwin-Strep-HAExpressionPlantMutationPromoter2 X 35SAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB402SN
Plasmid#224551PurposeEmpty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.DepositorTypeEmpty backboneUseTagsHA-Twin-StrepExpressionPlantMutationPromoter2 X 35SAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEVA421-Cas9tr
Plasmid#138709PurposePlasmid constitutively expressing the Cas9 nuclease and the non-coding tracrRNA from S. pyogenesDepositorInserttracrRNA-Cas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG017 (AVAST type 1)
Plasmid#157895PurposeExpresses the AVAST type 1 defense systemDepositorInsertAVAST type 1 (STAND ATPase with N-terminal serine protease)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRenilla
Plasmid#118059PurposeGBPart - Renilla Luciferase CDSDepositorInsertRenilla
UseLuciferase and Synthetic Biology; GbpartTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET11a-4SS-HEWL
Plasmid#39233DepositorInsertHen egg white lysozyme (LYZ Chicken)
UseTagsExpressionBacterialMutationN-terminal MetPromoterT7Available sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-N1-ChloC
Plasmid#85461PurposeHistorical plasmid. For inhibition of neuronal activity, #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)DepositorInsertChloC
UseTagsmCherryExpressionMammalianMutationE90R, T159CPromoterCMV (+enhancer)Available sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLucia
Plasmid#135287PurposeGBPart - Lucia Luciferase CDSDepositorInsertLucia Luciferase
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTE1062
Plasmid#172356PurposeDetection of Streptomyces coelicolor butyrolactones (SCB) by kanamycin bioassay. Streptomyces genomic integrative vector.DepositorInsertsUseSynthetic Biology; Integrative into streptomyces …TagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pELuc
Plasmid#118056PurposeGBPart - ELuc Luciferase CDSDepositorInsertELuc
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only