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pLenti PGK Blast DEST (w524-1)

Plasmid

#19065

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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AbVec1.1-IGKC

Plasmid

#80796

Purpose

Expression of immunoglobulin light chains in mammalian cells, human kappa constant region

Depositor

Hedda Wardemann

Publication

Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

Type

Empty backbone

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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1GFP/RNase H1 D210N

Plasmid

#174448

Purpose

Express GFP-tagged RNase H1 D210N in E. coli.

Depositor

Karlene Cimprich

Publication

Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

RNase H1 (RNASEH1 Human)

Tags

His-tag, GFP

Expression

Bacterial

Mutation

D210N

Promoter

T7 promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pBABEpuro-gateway

Plasmid

#51070

Purpose

gateway adapted mammalian expression vector

Depositor

Matthew Meyerson

Publication

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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Cbh_v5 AAV-ABE C-terminal

Plasmid

#137178

Purpose

AAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNA

Depositor

David Liu

Publication

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-CBE C-terminal; U6-protospacer

Use

AAV

Promoter

CMV

Available since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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FU-tetO-Gateway

Plasmid

#43914

Depositor

John Gearhart

Publication

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

6xHis and V5

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pCMV-hAR

Plasmid

#89078

Purpose

mammalian expression of human androgen receptor in pCMV5

Depositor

Elizabeth Wilson

Publication

Lubahn et al Science. 1988 Apr 15;240(4850):327-30.

Insert

human androgen receptor (hAR) full-length (AR Human)

Expression

Mammalian

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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MSCV PRDM16

Plasmid

#15504

Depositor

Bruce Spiegelman

Publication

Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

Insert

PRDM16 (isoform 4) (Prdm16 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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E[beta]C

Plasmid

#24312

Depositor

Roel Nusse

Publication

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

EF1alpha-betacatenin(deltaGSK) // SV40-mCherry (Ctnnb1 Mouse)

Use

Lentiviral

Mutation

Sites phosphorylated by GSK-3beta have been mutat…

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCDH-EF1-FHC

Plasmid

#64874

Purpose

lentiviral vector with Flag, HA tags

Depositor

Richard Wood

Publication

Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

Has service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

35 Articles

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pDONR207 SARS-CoV-2 M

Plasmid

#141274

Purpose

Gateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Has service

Cloning Grade DNA

Insert

M (M SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

6 Articles

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Flag-HA-USP22

Plasmid

#22575

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP22 (USP22 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDEST-CMV-N-EGFP

Plasmid

#122842

Purpose

N-terminal EGFP tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Publication

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway Cloning

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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GLUT1-eGFP/pcDNA-DEST47

Plasmid

#18729

Depositor

Wolf Frommer

Publication

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

pcDNA-DEST47 /hGLUT1-eGFP

Tags

GFP

Expression

Mammalian

Mutation

deleted TAA

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

Plasmid

#101060

Purpose

AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Publication

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Has service

AAV1

Insert

CaMPARI2

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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LentiV_Cas9_puro

Plasmid

#108100

Purpose

Lentiviral expression plasmid of spCas9 with puromycin resistance gene

Depositor

Christopher Vakoc

Publication

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS promoter

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

59 Articles

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pCMV-15F11-HA-mCh

Plasmid

#129591

Purpose

The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.

Depositor

Tim Stasevich

Publication

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-mCherry (15F11-HA scFv-mCh)

Expression

Mammalian

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pB-GLUT4-7myc-GFP

Plasmid

#52872

Purpose

detection and measurement of GLUT4 translocation

Depositor

Jonathan Bogan

Publication

Bogan et al Mol Cell Biol. 2001 Jul;21(14):4785-806.

Insert

GLUT4 (Slc2a4 Rat, with human c-terminus)

Use

Retroviral

Tags

7x-myc (internal) and GFP

Expression

Mammalian

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)

Plasmid

#101139

Purpose

Luciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.

Depositor

William Kaelin

Publication

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLVX-UbC-rtTA-Ngn2:2A:EGFP

Plasmid

#127288

Purpose

all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)

Depositor

Fred Gage

Publication

Schafer et al Nat Neurosci. 2019 Feb;22(2):243-255. do

Insert

Ngn2:2A:Ascl1 (NEUROG2 Human)

Use

Lentiviral

Tags

Ngn2 and EGFP linked via 2A

Expression

Mammalian

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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