We narrowed to 7,531 results for: Ski;

1. pMET17p-CCG 316

   Plasmid

   #131166

   Purpose

   For ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant

   Depositor

   Nils Johnsson

   Article

   Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:

   Type

   Empty backbone

   Tags

   CCG

   Expression

   Yeast

   Available Since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. LLhE_AW-pHG165 H118R

   Plasmid

   #90063

   Purpose

   Constitutive expression of a chimeric LacI:CelR transcription repressor

   Depositor

   Liskin Swint-Kruse

   Article

   Sousa et al J Mol Biol. 2016 Feb 22;428(4):671-8. do

   Insert

   LLhE_AW/H118R
   

   Expression

   Bacterial

   Mutation

   I48V/Q55A/Q60R/H118R

   Promoter

   Iq

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. LLhG-pHG165a

   Plasmid

   #90039

   Purpose

   Constitutive expression of a chimeric LacI:GalR transcription repressor

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Proteins. 2008 Dec;73(4):941-57. doi: 10

   Insert

   LLhG
   

   Expression

   Bacterial

   Promoter

   Iq

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. LGhG-pHG165a

   Plasmid

   #90048

   Purpose

   Constitutive expression of a chimeric LacI:GalR transcription repressor

   Depositor

   Liskin Swint-Kruse

   Article

   Tungtur et al J Mol Biol. 2010 Jan 29;395(4):785-802.

   Insert

   LGhG
   

   Expression

   Bacterial

   Promoter

   Iq

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. SMN2E7-Fluc-Nluc-reporter

   Plasmid

   #221005

   Purpose

   Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.

   Depositor

   Benjamin Blencowe, Mikko Taipale

   Article

   Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

   Insert

   Fluc-SMN2E7-STOP-Nluc
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. shh10

   Plasmid

   #64867

   Purpose

   This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

   Depositor

   John Flannery, David Schaffer

   Article

   Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

   Insert

   shh10 cap
   

   Use

   AAV

   Mutation

   I319V, N451D, D532N

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLJM1 EGFP-Tau

   Plasmid

   #108868

   Purpose

   Expresses EGFP linked to human Tau

   Depositor

   Gabriele Kaminski Schierle

   Insert

   Tau (MAPT Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   human four-repeat tau lacking the N-terminal sequ…

   Promoter

   CMV

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pV1382

   Plasmid

   #111436

   Purpose

   S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCSC-ASCL1-IRES-GFP-T2A-Sox11

   Plasmid

   #182233

   Purpose

   To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with LHX8, GBX1, FGF2 and two small molecules, forskolin and LDN-193189

   Depositor

   Chun-Li Zhang

   Article

   Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.

   Insert

   ASCL1-IRES-GFP-T2A-Sox11
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pV1326

    Plasmid

    #111435

    Purpose

    S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. GST-TACC3-His6

    Plasmid

    #69106

    Purpose

    For bacterial expression of recombinant human TACC3 tagged with GST and His.

    Depositor

    Stephen Royle

    Article

    Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

    Insert

    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    

    Tags

    GST and His6

    Expression

    Bacterial

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. PDX1-2A-eGFP

    Plasmid

    #66964

    Purpose

    PDX1-eGFP mini-vector donor for integration of the eGFP reporter into the PDX1 locus

    Depositor

    Danwei Huangfu

    Article

    Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

    Insert

    pancreatic and duodenal homeobox 1 (PDX1 Human)
    

    Use

    Unspecified

    Tags

    2A-eGFP

    Available Since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVG1

    Plasmid

    #111444

    Purpose

    Unified Solo vector pV1382 + sgScADE2 + ScADE2 stop codon repair tempate

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgScADE2/stop-codon repair
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3 T7 Jip1

    Plasmid

    #51699

    Purpose

    JIP-1 may suppress signal transduction by the JNK pathway"

    Depositor

    Roger Davis

    Article

    Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

    Insert

    Jnk interacting protein 1 (Mapk8ip1 Mouse)
    

    Tags

    T7

    Expression

    Mammalian

    Available Since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pSBtet-2xMARS-nGFP-puro

    Plasmid

    #205239

    Purpose

    DOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon system

    Depositor

    Jeremy Baskin

    Insert

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    

    Use

    Transposon

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    tight TRE promoter

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-2xMARS-nGFP-puro

    Plasmid

    #205238

    Purpose

    Lentiviral vector encoding two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody

    Depositor

    Jeremy Baskin

    Insert

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    

    Use

    Lentiviral

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV Syn ChR E90R-T159C 2A tDimer

    Plasmid

    #52495

    Purpose

    Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

    Depositor

    Thomas Oertner

    Article

    Wietek et al Science. 2014 Mar 27.

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    E90R, T159C

    Promoter

    human synapsin-1 and ribosomal skip sequence

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pV1464

    Plasmid

    #111440

    Purpose

    N. castellii Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMXs-Trib3

    Plasmid

    #131606

    Purpose

    expression of mouse Trib3 in mammalian cells

    Depositor

    Kateri Moore, Filipe Pereira

    Article

    Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

    Insert

    Trib3 (Trib3 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCSC-ASCL1-IRES-Sox11

    Plasmid

    #182234

    Purpose

    To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with LHX8, GBX1, FGF2 and two small molecules, forskolin and LDN-193189

    Depositor

    Chun-Li Zhang

    Article

    Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.

    Insert

    ASCL1-IRES-Sox11
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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