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pLenti-EF1a-Ly6G-P2A-PuroR-HA-miR30

Plasmid

#163359

Purpose

Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a Ly6G surface marker and a puromycin selection marker.

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

Ly-6G (Ly6g Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

EF1

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pET-22HT-MSI1(7-103)

Plasmid

#60357

Purpose

Express the RRM1 of mouse MSI1 in bacterial cells for protein purification

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-103) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

contains amino acids 7-103 of Msi1

Promoter

T7

Available Since

Nov. 24, 2014

Availability

Academic Institutions and Nonprofits only
attB-mCh-Dnmt3b1-Poly(A)-NeoR

Plasmid

#65553

Purpose

Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Neomycin selection

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Dnmt3b (Dnmt3b Mouse)

Use

Mouse Targeting; Bxb1

Tags

mCherry

Expression

Mammalian

Mutation

isoform 1

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pCAGGS/Daxx myc S502A

Plasmid

#19281

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

Daxx myc (Daxx Mouse)

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pBS-TRE-cofilin1(S3A)-P2A-mTurquoise2

Plasmid

#163610

Purpose

Expresses constitutively active cofilin and mTurquoise2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

cofilin1 (Cfl1 Mouse)

Tags

mTurquoise2

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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mGFAP R236H

Plasmid

#169475

Purpose

Single p.Arg236His(R236H) mutation was individually inserted by site-directed mutagenesis into the wild-type mGFAP

Depositor

Federico Herrera

Article

Letra-Vilela et al Experimental Results (2020), Volume 1, e

Insert

HaloTAG mGFAP (R236H) (Gfap Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Changed R 236 to H

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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ERAS

Plasmid

#55656

Purpose

expression of mouse ERAS

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

ERAS (Eras Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
JARID2-KO-gRNA-1

Plasmid

#131328

Purpose

gRNA to knockout JARID2. Use with JARID2-KO-gRNA-2

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

JARID2-KO-gRNA-1

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only
JARID2-KO-gRNA-2

Plasmid

#131327

Purpose

gRNA to knockout JARID2. Use with JARID2-KO-gRNA-1

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

JARID2-KO-gRNA-2

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only
TET-pLKO.1 PURO shId2 #1

Plasmid

#83088

Purpose

Lentiviral shRNA vector for inducible knockdown of mouse Id2

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shId2 (Id2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pFUSEss-CHIg-mG3_Lys322Arg

Plasmid

#105862

Purpose

Expression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Lys322Arg (EU numbering).

Depositor

Joanna Bereta

Article

Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

Insert

IgG3 heavy chain (Ighg3 Mouse)

Expression

Mammalian

Mutation

Lys322Arg in constant region of IgG1 heavy chain

Promoter

hEF1-HTLV prom

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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GEM

Plasmid

#55680

Purpose

expression of mouse GEM

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

GEM (Gem Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pmirGLO-Bmpr2-Δ19

Plasmid

#49381

Purpose

Insert is a fragment of the Bmpr2 3'-UTR that contains a mutated miR-19 binding site. To be used for 3'UTR assay.

Depositor

Martine Roussel

Article

Murphy et al Cancer Res. 2013 Oct 21.

Insert

Bmpr2 (BMPR2 Mouse, Human)

Use

Luciferase

Mutation

changed nucleotides TG to AC at bp 7344-7345

Promoter

Human phosphoglycerate kinase (PGK) promoter

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Pde2a

Plasmid

#105462

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2015 Mar;65:114-24. d

Insert

Pde2a (Pde2a Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1mLCAD_K42R_FLAG

Plasmid

#24486

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Long chain acyl-CoA dehydrogenase (Acadl Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Lys42Arg

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pLenti CRISPR V2 sgShmt2_1

Plasmid

#106314

Purpose

Express Cas9 and sgRNA targeting mShmt2

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting SHMT2 (Shmt1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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Dll3ICD-HPC4 pBacPAK8-3

Plasmid

#17334

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Delta-like 3 (Dll3 Mouse)

Tags

HPC4

Expression

Insect

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
mTalin2_R10

Plasmid

#111043

Purpose

Plasmid expresses the talin2 rod domain R10 a 5 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R9

Plasmid

#111042

Purpose

Plasmid expresses the talin2 rod domain R9 a 5 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pGL-Rbl1UTR

Plasmid

#20884

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

Rbl1 (p107) 3'UTR (Rbl1 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only