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pU6-Sp-gRNA-AAVS1_B3903c_attP

Plasmid

#182144

Purpose

To insert attP attachment site at AAVS1 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

AAVS1_B3903c_attP pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-AAVS1_A3786c_attP

Plasmid

#182143

Purpose

To insert attP attachment site at AAVS1 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

A3786c-attP pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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GFP shRNA tet pLKO puro

Plasmid

#163017

Purpose

Control tet-inducible shRNA targeting eGFP

Depositor

Mark Rubin

Article

Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

Insert

Enhanced Green Fluorescent Protein

Use

Lentiviral

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-LbNOX-FLAG

Plasmid

#218631

Purpose

This plasmid contains LbNOX under doxycycline inducible control.

Depositor

Valentin Cracan

Article

Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

Insert

LbNOX

Use

Lentiviral and Synthetic Biology

Tags

FLAG

Expression

Mammalian

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-CCR5_DF_B584b_attB

Plasmid

#182148

Purpose

To insert attB attachment site at CCR5 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

CCR5-B584b-attB pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-CCR5_DF_A509b_attB

Plasmid

#182147

Purpose

To insert attB attachment site at CCR5 in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

CCR5-A509b-attB pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_CalR-SNAP-tag2-KDEL

Plasmid

#226517

Purpose

Mamalian cell expression of SNAP-tag2 in endoplasmic reticulum

Depositor

Kai Johnsson

Article

Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

Insert

CalR-SNAP-tag2-KDEL

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pFUSE-DEST_R1R4

Plasmid

#97186

Purpose

For fusion gene cloning. Recombine Gene A + Gene B into this Gateway Destination Vector

Depositor

Kenneth Scott

Article

Lu et al Cancer Res. 2017 May 16. pii: canres.274

Type

Empty backbone

Use

Lentiviral; Gateway

Tags

V5

Expression

Mammalian

Promoter

EF1 alpha

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET-Cre-LPETG-6xHis

Plasmid

#237224

Purpose

Expresses LPETG-tagged Cre recombinase in bacteria

Depositor

Elliot Chaikof

Article

Eweje et al Nat Biotechnol. 2025 May 15. doi: 10.103

Insert

Cre-LPETG (cre Synthetic)

Tags

6xHis

Expression

Bacterial

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Glt1-eGFP

Plasmid

#176862

Purpose

Expresses Glt-1 fused with GFP at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Glt1-eGFP

Use

AAV

Tags

GFP

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-PELP1

Plasmid

#65208

Purpose

Full length mammalian expression vector for hPELP1

Depositor

Ratna Vadlamudi

Article

Nagpal et al Mol Cancer Res. 2008 May;6(5):851-61. do

Insert

PELP1 (PELP1 Human)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only
ShadowG-SUMO1

Plasmid

#175239

Purpose

Bacterial expression and purification, conjugatable SUMO for SUMOylation

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

SUMO1 (SUMO1 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV_SynP_DIO_tAKARα- T391A_WPRE

Plasmid

#196676

Purpose

AAV plasmid for mammalian Cre-dependent expression of the T391A mutant of tAKARα (abolished sensor phosphorylation site).

Depositor

Haining Zhong

Article

Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

Insert

tAKARα-T391A

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCarCBA

Plasmid

#117754

Purpose

Codon optimized CarCBA enzymes from Pectobacterium carotovorum in pBbaA5k backbone

Depositor

Greg Bokinsky

Article

Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

Insert

Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA)

Expression

Bacterial

Promoter

PLac(UV5)

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCarCBADE

Plasmid

#117757

Purpose

Codon optimized CarCBADE enzymes from Pectobacterium carotovorum in pBbaA5k backbone

Depositor

Greg Bokinsky

Article

Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

Insert

Codon optimised CarCBA, Protein CarD, Ferredoxin CarE

Expression

Bacterial

Promoter

PLac(UV5)

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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CP1028-ABE8e

Plasmid

#138492

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSpCas9 (CP1028)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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U6-5' MS2-CircRNA

Plasmid

#213750

Purpose

For circular RNA-mediated prime editor using U6-5' MS2-CircRNA in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

5' ribozyme, 5' ligation sequences, 5' MS2, 3' ligation sequences, 3' ribozyme

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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Mammalian expression: Z-lock cofilin dark state mutant

Plasmid

#141136

Purpose

a control for optogenetic cofilin analog

Depositor

Klaus Hahn

Article

Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

Insert

Zdk2, Cofilin, LOV2

Expression

Mammalian

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-SlugCas9-NNG-P2A-puro

Plasmid

#214352

Purpose

Expresses SlugCas9-NNG and puromycin resistance genes

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugCas9-NNG and puromycin resistance genes

Expression

Mammalian

Mutation

Q782R/S888R/L906R/N984S/E1012K/K1016I

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pNP2270

Plasmid

#222733

Purpose

pNP1950 - J23119-IS621 bRNA1 TBL-lacZ-1 (Extended RTG), DBL-WT, HDV; T7-IS621 Recombinase

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Insert

IS621 Helper (lacZ)

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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