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We narrowed to 15,952 results for: grna

Showing: 7441 - 7460 of 15952 results

  1. lentiCRISPRv2_PROX1_1+4

    Plasmid
    #121177
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Available Since
    March 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121177-plasmid-map-sequence-id-237896

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  2. pCASCADE-gltA2-udhA

    Plasmid
    #65819
    Purpose
    gltA-udhA targeting guide RNA array E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gltA2-udhA guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induc…
    Available Since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    65819-plasmid-map-sequence-id-111979

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  3. pX552-GFP

    Plasmid
    #107023
    Purpose
    pX552-CMV-GFP. AAV plasmid for sgRNA cloning, with GFP fusion facilitating FACS sorting of cells. Replaced the hSyn promoter in pX552 from Zhang lab with a CMV promoter.
    Depositor
    Alex Hewitt
    Insert
    GFP
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available Since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107023-plasmid-map-sequence-id-208521

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  4. pRDA_852

    Plasmid
    #185527
    Purpose
    Lentiviral vector that enables Cre-mediated expression of two sgRNAs. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR, Cre/Lox, and Lentiviral ; Flp/frt
    Expression
    Mammalian
    Promoter
    Human U6 and Mouse U6
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    185527-plasmid-map-sequence-id-366261

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  5. pCASCADE-gltA1-gltA2-GapAP1

    Plasmid
    #87150
    Purpose
    gltA1-gltA2-GapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gltA1-gltA2-gapAP1 guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87150-plasmid-map-sequence-id-174939

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  6. PB-UniSAM

    Plasmid
    #99866
    Purpose
    Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0
    Depositor
    Lesley Forrester
    Article
    Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.
    Insert
    UniSAM-mCherry + U6-gRNA2.0
    Use
    CRISPR and Synthetic Biology; Dcas9-sam activation
    Expression
    Mammalian
    Mutation
    BbsI sites in CDS were ablated by consensus mutat…
    Promoter
    EF1a
    Available Since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    99866-plasmid-map-sequence-id-192399

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  8. pHSE401

    Plasmid
    #62201
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    35S and AtU6-26p
    Available Since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62201-plasmid-map-sequence-id-228092

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  10. pHUE411

    Plasmid
    #62203
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    OsU3 and Ubi
    Available Since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62203-plasmid-map-sequence-id-467234

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  14. pBUE411

    Plasmid
    #62200
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    OsU3 and Ubi
    Available Since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62200-plasmid-map-sequence-id-395472

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  15. pBUN421

    Plasmid
    #62204
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    TaU3 and Ubi
    Available Since
    Feb. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    62204-plasmid-map-sequence-id-101817

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  20. pMOD_B2301

    Plasmid
    #91067
    Purpose
    Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    Use
    CRISPR
    Promoter
    35S
    Available Since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91067-plasmid-map-sequence-id-181873

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Showing: 7441 - 7460 of 15952 results