165,688 results

1. pcDNA -TMEM9B-3xHA

   Plasmid

   #225509

   Purpose

   TMEM9B expression vector

   Depositor

   Wade Harper

   Article

   Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

   Insert

   TMEM9B (TMEM9B Human)
   

   Tags

   3xHA

   Expression

   Mammalian

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EF1a-mCHERRY

   Plasmid

   #224298

   Purpose

   Expresses mCherry under EF1a promoter (CTRL vector)

   Depositor

   Nikhil Joshi

   Article

   Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

   Insert

   mCherry
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. DEXsynth:ccdb-eGFP + RUBY

   Plasmid

   #233317

   Purpose

   GATEWAY compatible vector with a dexamethasone (DEX)-inducible promoter driving in frame epitope fusion of eGFP paired with ccdb selection toxin and RUBY marker

   Depositor

   Thomas Baum

   Insert

   DEXsynth:ccdb-eGFP + RUBY
   

   Use

   Gateway destination

   Tags

   eGFP

   Available Since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAIP4_2ESK

   Plasmid

   #234232

   Purpose

   Heterologous protein expression of ubiquitin-conjugating enzyme E2 D2 in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   2ESK (UBE2D2 Human)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. DEXsynth:eGFP-ccdb + mcherry

   Plasmid

   #233311

   Purpose

   GATEWAY compatible vector with a dexamethasone (DEX)-inducible promoter driving in frame epitope fusion of eGFP paired with ccdb selection toxin and mCherry marker

   Depositor

   Thomas Baum

   Insert

   DEXsynth:eGFP-ccdb + mcherry
   

   Use

   Gateway destination

   Tags

   eGFP

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Tom20-ECFP-FRB

   Plasmid

   #210289

   Purpose

   mitochondrial OM marker and "anchor" in FKBP-FRB CID

   Depositor

   Takanari Inoue

   Article

   Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

   Insert

   Tom20-ECFP-FRB
   

   Tags

   ECFP and FRB

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEL1029

   Plasmid

   #225600

   Purpose

   The STU backbone vector for tomato genome editing is based on Mb3Cas12a-RRR (D172R/N573R/K579R)

   Depositor

   Yong Zhang

   Article

   Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA-pDEST_BirA_Flag

   Plasmid

   #227960

   Purpose

   BioID experiment

   Depositor

   Alessandro Ori

   Insert

   BirA
   

   Expression

   Mammalian

   Mutation

   R118G

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET26b_AT118-H-V5-His

   Plasmid

   #222850

   Purpose

   E. coli periplasmic expression plasmid encoding Nb. AT118-A, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-H
   

   Tags

   V5-His

   Expression

   Bacterial

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti puro HA-H2BC21

    Plasmid

    #227589

    Purpose

    Lentiviral plasmid expressing human H2BC21 protein

    Depositor

    Addgene Research Program, Melina Fan

    Insert

    H2BC21 (H2BC21 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    CMV

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti_CMV:Flag-CeNL

    Plasmid

    #208839

    Purpose

    Expresses FLAG epitope-tagged CeNL "Blue" BRET reporter in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

    Insert

    CeNL Nanolantern
    

    Use

    Lentiviral

    Tags

    1xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AA012

    Plasmid

    #215974

    Purpose

    Fragmid fragment: (N' terminus) Bipartite Nuclear Localization Signal

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Start; BPNLS_v1.1
    

    Use

    Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GRM6-DuET

    Plasmid

    #213304

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GRM6 (GRM6 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL0_29 [aph(3')-I EZ-Tn5]

    Plasmid

    #198942

    Purpose

    Level 0 part

    Depositor

    Cultivarium Tools, Nili Ostrov, Charlie Gilbert

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    aph(3')-I EZ-Tn5
    

    Use

    Synthetic Biology

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCBP60g-NLS-3xmCitrine

    Plasmid

    #192521

    Purpose

    Fluorescent reporter for plant responses to bacterial infection

    Depositor

    Gitta Coaker

    Article

    Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

    Insert

    CALMODULIN BINDING PROTEIN 60g
    

    Tags

    3xmCitrine and NLS

    Expression

    Plant

    Promoter

    CBP60g

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBAD33-Gm

    Plasmid

    #65098

    Purpose

    bacterial expression vector with Gentamycin selection, based on pBAD33

    Depositor

    Juan Tomas

    Article

    Jimenez et al J Bacteriol. 2009 Apr;191(7):2228-36. do

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmCherry-ATG5

    Plasmid

    #13095

    Depositor

    Roberta Gottlieb

    Article

    Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

    Insert

    ATG5 (Atg5 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Nov. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Circular-oc-EMCV-GFP

    Plasmid

    #226261

    Purpose

    Production of in vitro circularizable RNA via IVT

    Depositor

    Prashant Mali

    Article

    Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

    Insert

    EGFP
    

    Use

    In vitro transcription

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAM5188

    Plasmid

    #120087

    Purpose

    Cyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.

    Depositor

    Susan Golden

    Article

    Chen et al Microbiology. 2016 Dec;162(12):2029-2041

    Insert

    pAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
    

    Use

    Other

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTRIP-SFFV-EGFP-NLS

    Plasmid

    #86677

    Purpose

    Expresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events.

    Depositor

    Nicolas Manel

    Article

    Raab et al Science. 2016 Apr 15;352(6283):359-62. d

    Insert

    EGFP-NLS
    

    Use

    Lentiviral

    Available Since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDNA3.1-YY1

    Plasmid

    #169018

    Purpose

    Expression of YY1 driven by CMV promoter

    Depositor

    Claes Wadelius

    Article

    Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

    Insert

    human YY1 (YY1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCEP4-myc-HLA-A2

    Plasmid

    #135504

    Purpose

    Mammalian expression of myc-tagged HLA-A*02:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*02:01 (HLA-A Human)
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDB6 (CK2alpha')

    Plasmid

    #27084

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2alpha' (CSNK2A2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mScarlet-PPM1H

    Plasmid

    #233585

    Purpose

    Expresses PPM1H in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

    Insert

    PPM1H (PPM1H Human)
    

    Tags

    mScarlet

    Expression

    Mammalian

    Available Since

    June 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)

    Plasmid

    #31476

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV d14 GFP donor EF1s I-SceI
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Mutation

    GFP has 14 amino acids deleted from c terminus

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TRE-KRAB-dCas9-IRES-GFP

    Plasmid

    #85556

    Purpose

    2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFP

    Depositor

    Eric Lander

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Insert

    KRAB-dCas9-IRES-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA Tag, and KRAB domain

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti-HIFR

    Plasmid

    #192946

    Purpose

    Lentiviral production of HIF-1alpha reporter

    Depositor

    Markus Covert

    Article

    Jeknic et al J Biol Chem. 2023 Mar 10:104599. doi: 10

    Insert

    HIF-1alpha reporter
    

    Use

    Lentiviral

    Promoter

    mCMV

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AAV pCAG-FLEX-tdTomato-WPRE

    Plasmid

    #51503

    Purpose

    Can be used to generate AAV virus that will express tdTomato in the presence of Cre

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Insert

    tdTomato
    

    Use

    AAV

    Promoter

    pCAG

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. plenti-CAG-IKZF3-FLAG-IRES-GFP

    Plasmid

    #107388

    Purpose

    Lentiviral expression of IKZF3-FLAG

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF3 (IKZF3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mKeima-Red-C1

    Plasmid

    #54546

    Purpose

    Localization: C1 Cloning Vector, Excitation: 440, Emission: 620

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    mKeima-Red

    Expression

    Mammalian

    Available Since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge