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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 59,739 results for: Gene

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Showing: 11191 - 11220 of 59739 results

  14. pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139093
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dxCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139093-plasmid-map-sequence-id-273890

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  19. pcDNA3 mRuby2 LIC cloning vector (6H)

    Plasmid
    #86965
    Purpose
    Empty LIC vector; adds an mRuby2 gene to the C-terminus of your protein of interest.
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    mRuby2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86965-plasmid-map-sequence-id-174749

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  24. pEE531

    Plasmid
    #149290
    Purpose
    T-DNA encoding TRV2 with spacer multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2
    Depositor
    Daniel Voytas
    Article
    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41
    Insert
    p35S:TRV2_Spacer_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    Expression
    Plant
    Promoter
    35S
    Available Since
    July 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    149290-plasmid-map-sequence-id-305881

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  25. pEE491

    Plasmid
    #149292
    Purpose
    T-DNA encoding TRV2 with tRNA multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2
    Depositor
    Daniel Voytas
    Article
    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41
    Insert
    p35S:TRV2_tRNA_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    Expression
    Plant
    Promoter
    35S
    Available Since
    July 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    149292-plasmid-map-sequence-id-305826

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  27. pRAN473

    Plasmid
    #120813
    Purpose
    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target protein
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1
    Insert
    red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
    Expression
    Bacterial
    Promoter
    Ptet
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120813-plasmid-map-sequence-id-235308

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Showing: 11191 - 11220 of 59739 results