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NC15 pGLUE CUL3

Plasmid

#36970

Depositor

Randall Moon

Article

Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

Insert

CUL3 (CUL3 Human)

Tags

CBP tag, HA, and streptag

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2012

Availability

Academic Institutions and Nonprofits only
mEmerald-Mito-7

Plasmid

#54160

Purpose

Localization: Mitochondria, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

Mito (COX8A Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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mRuby2-Farnesyl-5

Plasmid

#55896

Purpose

Localization: Membrane, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Farnesyl (HRAS Human)

Tags

mRuby2

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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PB-PE

Plasmid

#162796

Purpose

All-in-one prime editor piggyBac transposon

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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miniSOG-Mito-7

Plasmid

#57773

Purpose

Localization: Mitochondria, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Mito (COX8A Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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miniSOG-LaminB1-10

Plasmid

#57771

Purpose

Localization: Nuclear Envelope, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

LaminB1 (LMNB1 Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE

Plasmid

#168106

Purpose

PiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloning

Depositor

Erich Jarvis

Article

Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

Insert

mCherry-KASH

Use

CRISPR and Cre/Lox

Promoter

CAG

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pIG-602_NY-ESO-1_TCR_RFP

Plasmid

#207483

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

RFP, NY-ESO-1_TCR

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-TCF21

Plasmid

#192894

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of TCF21

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

TCF21 (TCF21 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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HIV-1-GFP ΔEnv CA Q67H/N74D

Plasmid

#217441

Purpose

HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef. Contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with Q67H,N74D mutations in CA).

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

gag/pol

Use

Lentiviral

Mutation

CA Q67H,N74D

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pMH0001

Plasmid

#85969

Purpose

UCOE-SFFV-dCas9-BFP-KRAB

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Insert

dCas9

Use

Lentiviral

Tags

BFP-KRAB

Expression

Mammalian

Mutation

D10A, H840A

Promoter

SFFV

Available Since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-GRET

Plasmid

#158222

Purpose

Control lentiviral plasmid encoding GFP-Nluc BRET reporter from Schaub et al., Cancer Research, 2015.

Depositor

Charles P. Lai

Article

Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

Insert

GFP-Nluc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-SaPE2

Plasmid

#174817

Purpose

Mammalian expression of SaCas9 prime editor 2

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

SaPE2

Tags

SV40 bpNLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGD-P1/HC-Pro

Plasmid

#196328

Purpose

35S promoter-driven expression of the tobacco etch virus P1/HC-Pro RNA silencing suppressor

Depositor

Zhenghe Li

Article

Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

Insert

tobacco etch virus P1/HC-Pro

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pVax1-hVEGF165

Plasmid

#74466

Purpose

expression of hVEGF165 in mammalian cells

Depositor

Loree Heller

Article

Marshall WG et al Gene Ther. 2010 Mar;17(3):419-23. doi: 1

Insert

vascular endothelial growth factor 165 (VEGFA Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-HA-NEK7

Plasmid

#75142

Purpose

Expresses N-terminal HA-tagged NEK7 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pET20b-His6-LONP1

Plasmid

#247937

Purpose

Expression of human LONP1 with methionine 115 as its first residue (mature mitochondrial-matrix localized form)

Depositor

Gabriel Lander

Article

Shin et al Nat Commun. 2021 May 28;12(1):3239. doi:

Insert

LONP1 (LONP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

encodes aa 115-959

Promoter

T7

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Talin-TS

Plasmid

#83376

Purpose

FRET probe for measuring tension on Talin molecule

Depositor

Martin Schwartz

Article

Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

Insert

Talin1 (Tln1 Mouse)

Use

Retroviral

Tags

EGFP-F40-tagRFP (tension sensor module)

Expression

Mammalian

Mutation

Tension sensor module is inserted between V447 an…

Promoter

CMV

Available Since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only

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miniSOG-Actin-7

Plasmid

#57761

Purpose

Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Actin (ACTB Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pPB-cT3G-cERP2-NR1H2

Plasmid

#192919

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of NR1H2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

NR1H2 (NR1H2 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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