159,352 results

1. pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-mcherry)

   Plasmid

   #217306

   Purpose

   sgRNA mcherry + Puromycin

   Depositor

   Luke Gilbert

   Insert

   pU6-sgRNA, Ef1alpha-Puro-T2A-mcherry
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6, Ef1alpha

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF0662

   Plasmid

   #143063

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CHD6 (CHD6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. FRP792_insul-(lexA-box)2-PminCYC1-Citrine-TCYC1

   Plasmid

   #58433

   Purpose

   Induces Citrine expression from a promoter containing 2 lexA boxes in yeast cells

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   CitrineA206K
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   insul-(lexA-box)2-PminCYC1

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3

   Plasmid

   #189752

   Purpose

   Produces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysis

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mTurquoise2_spatial-linker-P2A_mScarlet3
   

   Use

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV & Rha

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSCD-TEVP

   Plasmid

   #226225

   Purpose

   Express TEVP

   Depositor

   Michael Elowitz

   Article

   Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

   Insert

   TEVP (TEVgp1 Tobacco etch virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET32a-HD46Q

   Plasmid

   #11515

   Depositor

   Pamela Bjorkman

   Article

   Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

   Insert

   huntingtin, exon 1, 46Q glutamines (HTT Human)
   

   Use

   Tags

   His, His (out of frame), and Trx

   Expression

   Bacterial

   Mutation

   huntingtin exon 1 with 46 glutamines, vector m…

   Promoter

   Available since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-mCherry-CaM

   Plasmid

   #127393

   Purpose

   Fluorescent reporter for calmodulin 1

   Depositor

   Ryohei Yasuda

   Article

   Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

   Insert

   Calmodulin 1 (Calm1 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIRESpuroN3xFLAG-His-CDK5RAP3

   Plasmid

   #204555

   Purpose

   Expresses 3xFLAG-His-tagged CDK5RAP3 in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   CDK5RAP3 (AMY2A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYES2-HTH-HHT2

   Plasmid

   #86482

   Purpose

   yeast expression of HHT2 with His10-TEV-HA tag

   Depositor

   Akash Gunjan

   Article

   Singh et al Nat Cell Biol. 2009 Aug;11(8):925-33. do

   Insert

   HHT2 (HHT2 Budding Yeast)
   

   Use

   Tags

   HIS10-TEV-HA (HTH)

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Jan. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. CFP-Parkin

    Plasmid

    #47560

    Purpose

    CFP tagged Parkin for colocalization study

    Depositor

    Richard Youle

    Article

    Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

    Insert

    Parkin (PRKN Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19/mChe-Cs

    Plasmid

    #183171

    Purpose

    Construct for transient expression of cytoskeleton-targeted mCherry in plants.

    Depositor

    Qinlong Zhu, Yao-Guang Liu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    mCherry-Cs
    

    Use

    Tags

    363 aa of A. thaliana FIMBRIN 1 (AtFIM1)

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPOTv7-blast-10Myc

    Plasmid

    #181939

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    10xMyc
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    read through

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJOG1336

    Plasmid

    #167303

    Purpose

    Level 0 module (3U+Ter) for further assemblies based on the Modular Cloning system. 3'UTR and flanking sequence of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Insert

    3'UTR and Terminator SlOLE2
    

    Use

    Synthetic Biology; Moclo level 0 vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJOG1334

    Plasmid

    #167302

    Purpose

    Level 0 module (Pro+5U) for further assemblies based on the Modular Cloning system. Promoter and 5'UTR of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Insert

    Promoter SlOLE2
    

    Use

    Synthetic Biology; Moclo level 0 vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETduet+p300HAT

    Plasmid

    #157793

    Purpose

    Expression of p300 HAT domain

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    p300HAT (EP300 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBabepuro-IL8

    Plasmid

    #189608

    Purpose

    Retroviral expression vector for IL8

    Depositor

    Thierry Tchenio

    Insert

    IL8 (CXCL8 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    SV40 early promoter

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19/mChe-Golgi

    Plasmid

    #183169

    Purpose

    Construct for transient expression of Golgi apparatus-targeted mCherry in plants.

    Depositor

    Qinlong Zhu, Yao-Guang Liu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    mCherry-Golgi
    

    Use

    Tags

    AtRER1B, A. thaliana endoplasmic reticulum retrie…

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmCherryC1 VAP-A

    Plasmid

    #226407

    Purpose

    Expression of human VAP-A fused to mCherry in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    VAP-A (VAPA Human)
    

    Use

    Tags

    T7-Xpress tag and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. AbVec2.0-IGHG3

    Plasmid

    #99577

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG3 isotype

    Depositor

    Hedda Wardemann

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSCV-BCL6-t2A-BCL2

    Plasmid

    #135305

    Purpose

    Overexpression of multiple human genes (BCL2 and BCL6)

    Depositor

    Daniel Hodson

    Article

    Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

    Inserts

    BCL6 (BCL6 Human)
    BCL2 (BCL2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    LTR

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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