165,307 results

1. pAAV-RAM-d2TTA::TRE-ChR2-WPREpA

   Plasmid

   #84471

   Purpose

   pAAV-RAM-ChR2

   Depositor

   Yingxi Lin

   Article

   Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

   Insert

   Channelrhodopsin 2
   

   Use

   AAV

   Tags

   EYFP

   Mutation

   H134R

   Promoter

   TRE

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIS1

   Plasmid

   #12179

   Depositor

   David Bartel

   Type

   Empty backbone

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWM_12x601_30bpLinker

   Plasmid

   #157789

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_30bp linker
   

   Expression

   Bacterial

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-N1 hMOG-alpha1-EGFP

   Plasmid

   #126463

   Purpose

   Expression a human MOG (isoform alpha1) EGFP fusion protein in mammalian cells

   Depositor

   Markus Reindl

   Article

   Mader et al J Neuroinflammation. 2011 Dec 28;8:184.

   Insert

   Myelin oligodendrocyte glycoprotein (MOG Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCENTR_aco_mScarlet3

   Plasmid

   #225984

   Purpose

   Arabidopsis codon-optimized mScarlet3 in an entry clone

   Depositor

   Imran Siddiqi

   Insert

   mScarlet3 Arabidopsis codon-optimized
   

   Use

   Synthetic Biology

   Mutation

   Arabidopsis Codon-Optimized mScarlet3

   Available Since

   Oct. 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAAV-hSyn-Cre-P2A-dTomato

   Plasmid

   #107738

   Purpose

   Can be used to express Cre recombinase and simultaneously the red fluorescent protein dTomato. Can also be used to create adeno-associated virus for delivery of these genes.

   Depositor

   Rylan Larsen

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   Cre, dTomato
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-∆Hapln1

   Plasmid

   #200778

   Purpose

   Expresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoter

   Depositor

   Alexander Dityatev

   Article

   Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

   Insert

   Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
   

   Use

   AAV

   Tags

   cfGFP

   Mutation

   deleted lectican binding domain (aminoacids 40-15…

   Promoter

   Synapsin

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOGG001

   Plasmid

   #113978

   Purpose

   Level 0 module. nptII promoter for constitutive gene expression

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   nptII promoter
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA6a-GST-TRP1

   Plasmid

   #41603

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   TRP1 (TRP1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   GST and yeast ADH1 terminator

   Available Since

   Dec. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-ClopHensor

    Plasmid

    #25938

    Depositor

    Daniele Arosio

    Article

    Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

    Insert

    ClopHensor
    

    Tags

    Strep-II

    Expression

    Mammalian

    Mutation

    E2GFP is EGFP-T203Y

    Available Since

    Sept. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJSK659

    Plasmid

    #205128

    Purpose

    Expression of GFP-SnxA in mammalian cells

    Depositor

    Jason King

    Article

    Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

    Insert

    SnxA
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1 shRUNX1 puro

    Plasmid

    #45816

    Depositor

    Kevin Janes

    Article

    Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

    Insert

    Runx1 (RUNX1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. HsTPC2-EYFP

    Plasmid

    #135194

    Purpose

    Expresses tagged TPC2 in mammalian cells

    Depositor

    Antony Galione

    Article

    Davis et al Curr Biol. 2012 Dec 18;22(24):2331-7. do

    Insert

    TPC2 (TPCN2 Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCdrA-gfpC

    Plasmid

    #111614

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(Mut3)-T0-T1
    

    Expression

    Bacterial

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBa.TfR.GFP

    Plasmid

    #45060

    Depositor

    Gary Banker, Marvin Bentley

    Article

    Burack et al Neuron. 2000 May;26(2):465-72.

    Insert

    TfR (TFRC Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    Chicken Beta Actin

    Available Since

    Oct. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. Gq-CASE

    Plasmid

    #168125

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gq. Composed of the subunits G alpha q (GNAQ) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAQ, GNB3, GNG9 (GNAQ, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at F124/E125 within GNAQ

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFA6a-GBP-RFP-kanMX6

    Plasmid

    #105160

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

    Insert

    GBP-RFP-kanMX6
    

    Tags

    GBP-RFP

    Expression

    Bacterial and Yeast

    Available Since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1 - TRC mTagBFP2

    Plasmid

    #191566

    Purpose

    Expresses an shRNA and mTagBFP2

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6 for shRNA

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-RTP1S

    Plasmid

    #149364

    Purpose

    Expresses RTP1S in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Inagaki et al Cell Rep. 2020 Jun 30;31(13):107814. doi

    Insert

    RTP1S (Rtp1 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pML104-KanMx4

    Plasmid

    #83476

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    KanMX4
    

    Expression

    Yeast

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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