19,660 results

1. GFP-PTEN

   Plasmid

   #13039

   Depositor

   Alonzo Ross

   Article

   Liu et al J Cell Biochem. 2005 Oct 1. 96(2):221-34

   Insert

   PTEN (PTEN Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLZRS-IRES-deltaNGFR

   Plasmid

   #72930

   Purpose

   retroviral expression with FACS-selectable marker (extracellular part of NGFR)

   Depositor

   Esther van de Vosse

   Article

   van_de_Vosse et al Blood. 2009 May 7;113(19):4548-55. doi:

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIND-STAT1-V5

   Plasmid

   #11618

   Depositor

   Steven Johnson

   Insert

   Signal transducer and activator of transcription 1 (STAT1 Human)
   

   Use

   Ecdysone inducible

   Tags

   V5

   Expression

   Mammalian

   Mutation

   no mutations

   Available Since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pLEX307-SARS-CoV-2-3CL

   Plasmid

   #160278

   Purpose

   Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. hMLKL-Venus

   Plasmid

   #106078

   Purpose

   expression in mammalian cells

   Depositor

   Douglas Green

   Article

   Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

   Insert

   human MLKL-Venus (MLKL Human)
   

   Use

   Retroviral; Tet-on retrovirus

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   Dox-inducible

   Available Since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pR_LG lenti-(empty)-del-CTS

   Plasmid

   #112895

   Purpose

   Non-integrating lentiviral co-packaging plasmid designed to reduce multiple integrations and recombination during transduction of nucleic acid libraries

   Depositor

   Paul Blainey

   Article

   Feldman et al bioRxiv 262121

   Type

   Empty backbone

   Use

   Lentiviral

   Promoter

   none

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-ZFN-AAVS1

   Plasmid

   #89707

   Purpose

   Mammalian expression plasmid for zinc finger nuclease targeting human AAVS1 locus

   Depositor

   Dmitriy Mazurov

   Article

   Zotova et al Viruses. 2017 Nov 2;9(11). pii: v9110325

   Insert

   ZFN-AAVS1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. 7a sgRNA for EJ7-GFP and 4-μHOM reporters

   Plasmid

   #113620

   Purpose

   sgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reporters

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   7a sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

9. PGK1p-Csy4-pA (Construct 2)

   Plasmid

   #55196

   Purpose

   Expresses Csy4 in Mammalian cells for site-specific RNA processing

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   Csy4/Cas6
   

   Use

   Synthetic Biology

   Tags

   6xHIS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVCT-tTR-KRAB

    Plasmid

    #11643

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    CAG promoters, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-p2A-CreERT2-FRT-kan-FRT

    Plasmid

    #108454

    Purpose

    This plasmid can be used to recombine the mCherry-p2a-CreERT2-pA cassette (or only CreERT2-pA) into a BAC. Also to produce mCherry-p2A-CreERT2 mRNA using SP6.

    Depositor

    Nadia Mercader Huber

    Article

    Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

    Insert

    mCherry-p2a-CreERT2
    

    Use

    Cre/Lox

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. p3xFLAG-EYFP

    Plasmid

    #34582

    Depositor

    Celeste Nelson

    Article

    Lee et al EMBO J. 2011 May 24;30(13):2662-74. doi:

    Type

    Empty backbone

    Tags

    3xFLAG and EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV 3xFLAG-GABARAP WT

    Plasmid

    #123097

    Purpose

    Expresses 3xFLAG-GABARAP wild-type in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX4T3-GST-Tev

    Plasmid

    #177142

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of the Tev protease

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    Tev
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. eGFP L202 Reporter

    Plasmid

    #119129

    Purpose

    Acts as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter.

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    mCherry and eGFP
    

    Use

    Lentiviral

    Mutation

    eGFP L202S

    Promoter

    CMV

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAJM.773

    Plasmid

    #108527

    Purpose

    VanRAM + PVanCC-YFP Reporter. Van sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    VanRAM + PVanCC-YFP
    

    Expression

    Bacterial

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEB2-mScarlet

    Plasmid

    #104006

    Purpose

    Plasmid encoding mScarlet

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mScarlet
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. FLT3-MSCV-IRES-GFP

    Plasmid

    #184778

    Purpose

    Expresses wildtype FLT3 in mammalian cells

    Depositor

    Timothy Ley

    Insert

    FLT3 (FLT3 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. p4489 Flag-betaTrCP

    Plasmid

    #10865

    Depositor

    Peter Howley

    Article

    Zhou et al Mol Cell. 2000 Sep . 6(3):751-6.

    Insert

    betaTrCP (BTRC Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-N-Myc_C223S USP7

    Plasmid

    #131243

    Purpose

    Mammalian expression of catalytically inactive Myc-tagged USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Contains a point mutation that converts USP7 cyst…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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