We narrowed to 11,840 results for: cel.2

1. pEGFP-hGATE-16

   Plasmid

   #87870

   Purpose

   Express a EGFP-human GATE-16 in mammalian cells

   Depositor

   Eiki Kominami, Isei Tanida

   Article

   Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

   Insert

   GATE-16 (GABARAPL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-NLC-DHFR-SpCas9

   Plasmid

   #124523

   Purpose

   Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

   Insert

   NLC-DHFR-SpCas9
   

   Use

   AAV

   Tags

   Destabilized domain of E.coli dihydrofolate reduc…

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-N1 VAMP2 R125TAG

   Plasmid

   #69876

   Purpose

   express in mammalian cells for unnatural amino acid incorporation into VAMP2-GFP

   Depositor

   Silvio Rizzoli

   Article

   Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

   Insert

   VAMP2-GFP (Vamp2 Rat)
   

   Use

   Synthetic Biology

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Amber stop codon in the linker between VAMP2 and …

   Promoter

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pPB-tetpA-hNEUROG2-iRPT

   Plasmid

   #140764

   Purpose

   Expresses iRFP713, PuroR and rtTAM2 in mammalian cells, with TRE-hNEUROG2

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Neurosci Res. 2019 Oct 3. pii: S0168-010

   Inserts

   iRFP713
   PuroR
   rtTAM2
   NEUROG2 (NEUROG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pN1 VAMP2 R125TAG

   Plasmid

   #69877

   Purpose

   express in mammalian cells for unnatural amino acid incorporation into VAMP2

   Depositor

   Silvio Rizzoli

   Article

   Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

   Insert

   VAMP2 (Vamp2 Rat)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Amber and Ochre stop codons in the former linker …

   Promoter

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pCSIIneo-TRE-hPhyB621- mCherry-HRasCT

   Plasmid

   #139482

   Purpose

   Expresses PhyB621-mCherry-HRasCT in mammalian cells.

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

   Insert

   PhyB621 (PHYB Mustard Weed)
   

   Use

   Lentiviral

   Tags

   mCherry-HRasCT

   Expression

   Mammalian

   Mutation

   G229L and deletion of aa Y at position 235 in mch…

   Promoter

   Tet responsible element

   Available since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac-M13-8xHis-ZZ-LTLT-HOOK3

   Plasmid

   #222287

   Purpose

   Expression of HOOK3 in insect cells for purification of untagged adapter (8xHIS-ZZtag can be cleaved off with TEV protease).

   Depositor

   Anne Straube

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Insert

   HOOK3 (HOOK3 Human)
   

   Use

   Tags

   8 His and ZZ tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYO1015

   Plasmid

   #235704

   Purpose

   Expression of FL human VPS15-ZZ in mammalian cells

   Depositor

   Roger Williams

   Article

   Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

   Insert

   VPS15 (PIK3R4 Human)
   

   Use

   Tags

   3xTEV-ZZ

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYO350

   Plasmid

   #235717

   Purpose

   Expression of FL human VPS15 in mammalian cells

   Depositor

   Roger Williams

   Article

   Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

   Insert

   VPS15 (PIK3R4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYO1005

    Plasmid

    #235702

    Purpose

    Expression of FL human UVRAG in mammalian cells

    Depositor

    Roger Williams

    Article

    Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

    Insert

    UVRAG (UVRAG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYO1274

    Plasmid

    #235709

    Purpose

    Expression of FL human VPS34 REIE > AAAA mutant in mammalian cells

    Depositor

    Roger Williams

    Article

    Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

    Insert

    VPS34 (PIK3C3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-FKBP-mEGFP-WIPI2 IDR (364-425aa)

    Plasmid

    #223758

    Purpose

    Stable expression and inducible recruitment of protein in mammalian cell culture

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    WIPI2 IDR (WIPI2 Human)
    

    Use

    Lentiviral

    Tags

    FKBP-mEGFP

    Expression

    Mutation

    aa 364-425 only

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218)

    Plasmid

    #229819

    Purpose

    A gateway compatible Tol2 destination vector containing the fli1a promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTol2-DEST-hsp70-zCreI-BFP (JDW 1184)

    Plasmid

    #229831

    Purpose

    A gateway compatible Tol2 destination vector containing the hsp70 promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMRXIP-GFP-TAPP1(PHD)

    Plasmid

    #221757

    Purpose

    Stable expression of GFP-TAPP1(PHD) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    PLEKHA1 (PLEKHA1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    tandem TAPP1 (PHD) (amino acids 184-304)

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-RepoMan-T394A

    Plasmid

    #204994

    Purpose

    Expresses shRNA resistant GFP-RepoMan T394A Mutant

    Depositor

    Lienhard Schmitz

    Article

    Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

    Insert

    cell devision cycle associated 2 (CDCA2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    changed Threonine 394 to Alanin

    Promoter

    CMV

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP-RepoMan-RATA

    Plasmid

    #184270

    Purpose

    Expresses shRNA resistant GFP-RepoMan RATA Mutant

    Depositor

    Lienhard Schmitz

    Article

    Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

    Insert

    cell division cycle associated 2 (CDCA2 Human)
    

    Use

    Tags

    3xHA and GFP

    Expression

    Mammalian

    Mutation

    V383A, F395A

    Promoter

    CMV

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MC2R-DuET

    Plasmid

    #213336

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MC2R (MC2R Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. F2RL2-DuET

    Plasmid

    #213236

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    F2RL2 (F2RL2 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CCRL2-DuET

    Plasmid

    #213205

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CCRL2 (CCRL2 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AVPR2-DuET

    Plasmid

    #213188

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    AVPR2 (AVPR2 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAGGS-AnkB ZU5ex-3xHA

    Plasmid

    #213732

    Purpose

    Mammalian cell expression vector for overexpression of AnkB ZU5ex isoform in motor neurons.

    Depositor

    Chaolin Zhang

    Article

    Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

    Insert

    AnkB ZU5ex (Ank2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE2-TetO-mSox2C17

    Plasmid

    #206369

    Purpose

    Expresses mouse SOX2-C17 in mammalian cells, for lentivirus generation.

    Depositor

    Ralf Jauch

    Article

    Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

    Insert

    Sox2-C17 chimera (Sox2, Sox17 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. eSpCas9-EF-5Flnc

    Plasmid

    #174870

    Purpose

    CRISPR vector for generating Flnc STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Flnc (Flnc Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1 Flag YFP hNKCC1 P676C I730C (NT809)

    Plasmid

    #50866

    Purpose

    Expresses human NKCC1 P676C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    P676C I730C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1 Flag YFP hNKCC1 I674C (NT475)

    Plasmid

    #50875

    Purpose

    Expresses human NKCC1 I674C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    I674C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1 Flag YFP hNKCC1 N680C A734C (NT816)

    Plasmid

    #50870

    Purpose

    Expresses human NKCC1 N680C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    N680C A734C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1 Flag YFP hNKCC1 L737C (NT523)

    Plasmid

    #50891

    Purpose

    Expresses human NKCC1 L737C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    L737C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1 Flag YFP hNKCC1 P676C A734C (NT810)

    Plasmid

    #50867

    Purpose

    Expresses human NKCC1 P676C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    P676C A734C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1 Flag YFP hNKCC1 N672C (NT474)

    Plasmid

    #50873

    Purpose

    Expresses human NKCC1 N672C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    N672C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge