12,469 results

1. sg2

   Plasmid

   #113967

   Purpose

   Single short guide RNA targeting TACCACATTTGTAGAGGTT

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   sg2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. sg3

   Plasmid

   #113968

   Purpose

   Single short guide RNA targeting CAATGTATCTTATCATGTC

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   sg3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. sg2+sg3

   Plasmid

   #113970

   Purpose

   Double short guide RNA targeting TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   sg2+sg3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMBP-parallel1-GK5_v2 (D30N+D280N+D443N)

   Plasmid

   #134291

   Purpose

   Expresses GK5 (isoform 2)-D30N/D280N/D443N mutant in bacteria

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   Gk5 (Gk5 Mouse)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed Aspartic Acids 30, 280 and 443 to Asparag…

   Promoter

   T7

   Available since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBOB-N-3HA-GK_v2

   Plasmid

   #134277

   Purpose

   Lentivector encoding 3XHA-tagged GK (isoform 2)

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   Gk (Gk Mouse)
   

   Use

   Lentiviral

   Tags

   3X HA

   Expression

   Mammalian

   Mutation

   553 amino acids, isoform 2

   Promoter

   CMV

   Available since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBOB-N-Flag-GK (281-473)

   Plasmid

   #134273

   Purpose

   Lentivector encoding Flag-tagged GK truncation (amino acids 281-473)

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   Gk (Gk Mouse)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   mouse Gk amino acids 281-473

   Promoter

   CMV

   Available since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBOB-N-Flag-GK (12-272)

   Plasmid

   #134272

   Purpose

   Lentivector encoding Flag-tagged GK truncation (amino acids 12-272)

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   Gk (Gk Mouse)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   mouse Gk amino acids 12-272

   Promoter

   CMV

   Available since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBOB-N-Flag-GK_v1

   Plasmid

   #134270

   Purpose

   Lentivector encoding Flag-tagged GK (isoform 1)

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   Gk (Gk Mouse)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   mouse Gk isoform 1; 524 amino acids

   Promoter

   CMV

   Available since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRDAX-VALENTIA-005 Lyn-Neptune//BsaI

   Plasmid

   #130186

   Purpose

   EMMA Kit # 1000000119 extension

   Depositor

   Gary Lewin

   Insert

   Lyn-Neptune
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRDAX-VALENTIA-010 LifeAct-Neptune//BsaI

    Plasmid

    #130191

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    LifeAct-Neptune
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRDAX-VALENTIA-015 Vimentin-Neptune//BsaI

    Plasmid

    #130196

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    Vimentin-Neptune
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRDAX-VALENTIA-016 NLSNoLS-Sirius//BsaI

    Plasmid

    #130197

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    NLSNoLS-Sirius
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRDAX-VALENTIA-017 NLSNoLS-SuperNova//BsaI

    Plasmid

    #130198

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    NLSNoLS-SuperNova
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRDAX-VALENTIA-033 NLSNoLS-Eos//BsaI

    Plasmid

    #130214

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    NLSNoLS-Eos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRDAX-615 C-Tag Venus210[1] L20 Stat3(R609Q) EBPF2

    Plasmid

    #130090

    Purpose

    PPI (BiFC/TriFC) assay vector

    Depositor

    Gary Lewin

    Insert

    Stat3(R609Q)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R609Q

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRDAX-614 N-Tag Venus210[1] L10 Stat3(R609Q) EBPF2

    Plasmid

    #130089

    Purpose

    PPI (BiFC/TriFC) assay vector

    Depositor

    Gary Lewin

    Insert

    Stat3(R609Q)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R609Q

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRDAX-611 C-Tag Venus210[2] L32 Stat3 mCherry

    Plasmid

    #130086

    Purpose

    PPI (BiFC/TriFC) assay vector

    Depositor

    Gary Lewin

    Insert

    Stat3 (Stat3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRDAX-603 C-Tag Venus210[1] L20 Stat3 EBPF2

    Plasmid

    #130078

    Purpose

    PPI (BiFC/TriFC) assay vector

    Depositor

    Gary Lewin

    Insert

    Stat3 (Stat3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRDAX-527 C-Tag GFP[1] L32 Tln1(rod) mCherry

    Plasmid

    #130054

    Purpose

    PPI (BiFC/TriFC) assay vector

    Depositor

    Gary Lewin

    Insert

    Tln1 rod
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRDAX-526 N-Tag GFP[1] L20 Tln1(rod) mCherry

    Plasmid

    #130053

    Purpose

    PPI (BiFC/TriFC) assay vector

    Depositor

    Gary Lewin

    Insert

    Tln1 rod
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBOB-C-Flag-GK5-toku (1-174)

    Plasmid

    #134261

    Purpose

    Lentivector encoding Flag-tagged toku form of GK5 (amino acids 1-174)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse Gk5 amino acids 1-174

    Promoter

    CMV

    Available since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Antares-SEPLuc IRESv24

    Plasmid

    #141087

    Purpose

    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-IRESv24-SEPLuc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mPLD2-PX (62-192)

    Plasmid

    #119128

    Purpose

    Bacterial expression of human phox homology (PX) domain, mPLD2-PX (62-192)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    mPLD2-PX (62-192)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Y365A or A365Y inducible rescue

    Plasmid

    #131323

    Purpose

    gRNA to generate i-WT-r or i-MT-r systems

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    Y365A or A365Y inducible rescue
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSECC-sgSIK1-B

    Plasmid

    #138666

    Purpose

    Expresses a mouse SIK1-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK1 mouse (Sik1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMBP-parallel1-GK5_v2 (D443N)

    Plasmid

    #134293

    Purpose

    Expresses GK5 (isoform 2)-D443N mutant in bacteria

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed Aspartic Acid 443 to Asparagine

    Promoter

    T7

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMBP-parallel1-GK5_v2 (D30N+D280N)

    Plasmid

    #134292

    Purpose

    Expresses GK5 (isoform 2)-D30N/D280N mutant in bacteria

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed Aspartic Acids 30 and 280 to Asparagine

    Promoter

    T7

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMBP-parallel1-GK5_v2 (D280N)

    Plasmid

    #134290

    Purpose

    Expresses GK5 (isoform 2)-D280N mutant in bacteria

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed Aspartic Acid 280 to Asparagine

    Promoter

    T7

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMBP-parallel1-GK5_v2 (D30N)

    Plasmid

    #134289

    Purpose

    Expresses GK5 (isoform 2)-D30N mutant in bacteria

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed Aspartic Acid 30 to Asparagine

    Promoter

    T7

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA6-C-Flag-GK5_v1

    Plasmid

    #134281

    Purpose

    Expresses Flag-tagged GK5 (isoform 1) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse Gk5 isoform 1; 516 amino acids

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge