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We narrowed to 13,072 results for: BASE;

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Showing: 7501 - 7520 of 13072 results

  2. pSW002-Pc-TorT(sp)-mNeonGreen

    Plasmid
    #205021
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    TorT-mNeonGreen
    Expression
    Bacterial
    Mutation
    mNeonGreen is codon optimized for expression in P…
    Promoter
    Pc
    Available Since
    Sept. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    205021-plasmid-map-sequence-id-407999

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  5. pNOC_dCas9_sgRNA NC

    Plasmid
    #176259
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176259-plasmid-map-sequence-id-346581

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  6. pdCas9 (GB1079)

    Plasmid
    #75399
    Purpose
    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusions
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75399-plasmid-map-sequence-id-151817

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  7. p426_Cas9_gRNA-ARS106a

    Plasmid
    #87382
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS106a sequence ATACGGTCAGGGTAGCGCCC in yeast chromosome 1.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS106a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87382-plasmid-map-sequence-id-193424

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  9. LF701: pMAGIC (L1-R5) hU6::xCas9(3.7) gRNA scaffold

    Plasmid
    #121817
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121817-plasmid-map-sequence-id-237365

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  11. pRD421

    Plasmid
    #163112
    Purpose
    Production of His6_TEV-cleavable-linker_mEos3.2_H-NSdbd in BL21 (DE3) pLysS
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    His-tag_TEV-cleavable-linker_mEos3.2_H-NSdbd
    Tags
    His-tag with TEV-cleavable linker
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163112-plasmid-map-sequence-id-321831

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  13. tRNA-gRNA position [M1_n-1] (GB1210)

    Plasmid
    #75411
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_n-1]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (2-part multiplexing)
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    tRNA-gRNA position [M1_n-1]
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75411-plasmid-map-sequence-id-211947

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  15. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N

    Plasmid
    #113952
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, I132S and S212N su…
    Promoter
    cmv
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113952-plasmid-map-sequence-id-215166

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  18. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N-D231G

    Plasmid
    #113953
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, I132S, S212N, and …
    Promoter
    cmv
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113953-plasmid-map-sequence-id-215167

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  20. p426_Cas9_gRNA-ARS1114a

    Plasmid
    #87397
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1114a sequence CTTGTGAAACAAATAATTGG in yeast chromosome 11.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1114a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87397-plasmid-map-sequence-id-193440

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Showing: 7501 - 7520 of 13072 results