We narrowed to 11,713 results for: NSI

1. pCMV7.1_3xFLAG-LATS1

   Plasmid

   #172986

   Purpose

   constitutive expression of FLAG-tagged LATS1

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   3xFLAG-LATS1 (LATS1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX_TRC311 ETV4-L

   Plasmid

   #74982

   Purpose

   Constitutive ETV4 ORF, blasticidin selection

   Depositor

   William Hahn

   Article

   Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

   Insert

   ETV4 (ETV4 Human)
   

   Expression

   Mammalian

   Mutation

   S396N

   Promoter

   EF1a

   Available Since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. His-MBP human SPOP (aa 28-337)

   Plasmid

   #52294

   Purpose

   bacterial expression of human SPOP (aa 28-337) fused to His-MBP

   Depositor

   Brenda Schulman

   Article

   Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

   Insert

   SPOP (SPOP Human)
   

   Tags

   His-MBP and TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 11, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pCDNA3-EMTB-TurboID-V5

   Plasmid

   #190736

   Purpose

   Mammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

   Depositor

   Jeremy Baskin

   Article

   Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

   Insert

   ensconsin (MAP7 Human)
   

   Tags

   Myc, TurboID, and V5

   Expression

   Mammalian

   Mutation

   Microtubule binding-domain of ensconsin (amino ac…

   Promoter

   CMV

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX302_HOXB13

   Plasmid

   #70089

   Purpose

   Expression of human HOXB13 from CMV promoter, puromycin selection marker

   Depositor

   William Hahn

   Article

   Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

   Insert

   HOXB13 (HOXB13 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQ54-VC

   Plasmid

   #175060

   Purpose

   expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ54) in mammalian cells

   Depositor

   Arnold Boersma

   Article

   Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

   Insert

   polyQ54 (HTT Human)
   

   Tags

   mVenus + mCherry

   Expression

   Mammalian

   Mutation

   mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

   Promoter

   CMV Promoter

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQ25-VC

   Plasmid

   #175062

   Purpose

   expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ25) in mammalian cells

   Depositor

   Arnold Boersma

   Article

   Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

   Insert

   polyQ25 (HTT Human)
   

   Tags

   mVenus + mCherry

   Expression

   Mammalian

   Mutation

   mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

   Promoter

   CMV Promoter

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW-Cdt1-mCherry-Puro

   Plasmid

   #193763

   Purpose

   Doxycyline-inducible (TetOn) human Cdt1 (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   Cdt1-mCherry (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   WT

   Mutation

   human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

   Promoter

   TRE

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. LCV2 IRF3 KO

   Plasmid

   #217446

   Purpose

   Lentiviral vector expressing Cas9 and an sgRNA targeting human IRF3

   Depositor

   Jarrod Johnson

   Article

   Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

   Insert

   sgRNA targeting IRF3 (IRF3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC1-dmito-oROS-HT

    Plasmid

    #216418

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to mitochondrial matrix.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    dmito-oROS-HT
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_HCK-1/1

    Plasmid

    #91273

    Purpose

    Protein expression and purification of human SH3 domain construct HCK-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    HCK-1/1 (HCK Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQ39-VC

    Plasmid

    #175061

    Purpose

    expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ39) in mammalian cells

    Depositor

    Arnold Boersma

    Article

    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

    Insert

    polyQ39 (HTT Human)
    

    Tags

    mVenus + mCherry

    Expression

    Mammalian

    Mutation

    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

    Promoter

    CMV Promoter

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. INS-2A-luciferase-2A-CD19-GFP

    Plasmid

    #159349

    Purpose

    Human INS targeting vector for knockin of 2A separated luciferase, humand CD19, and GFP. Targeted cells will be puromycin resistant.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

    Inserts

    Luciferase-2A-human_CD19-2A-GFP
    Luciferase-2A-human_CD19-2A-GFP (CD19 Human)
    

    Use

    Ins locus knockin donor vector

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW-Cdt1ΔPIP-mCherry-Puro

    Plasmid

    #193765

    Purpose

    Doxycyline-inducible (TetOn) human Cdt1ΔPIP (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    Cdt1ΔPIP-mCherry (CDT1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Mutation

    PIP degron removed (a.a.1-19 removed)

    Promoter

    TRE

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMA7-SacB

    Plasmid

    #79968

    Purpose

    TM-MAGE strain

    Depositor

    Morten Sommer

    Article

    Lennen et al Nucleic Acids Res. 2016 Feb 29;44(4):e36

    Inserts

    Lambda Red recombinase beta subunit
    DNA adenine methylase
    Levansucrase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Epac-S-H81

    Plasmid

    #170339

    Purpose

    GFPDel(UST)_EPACdDEPCD_mRFP biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

    Insert

    GFPDel(UST)_EPACdDEPCD_mRFP (RAPGEF3 Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available Since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX6p1-GST-Rnf31

    Plasmid

    #63134

    Purpose

    Expression of human RNF31/HOIP codon optimized for E. coli and insect cells

    Depositor

    Titia Sixma

    Article

    Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:

    Insert

    Rnf31 (RNF31 Synthetic, Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Synthetic based on human protein sequence

    Promoter

    tac

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

18. UPB-esophagus (UPB5)

    Plasmid

    #180783

    Purpose

    Unique Projection Barcode (UPB5)-Esophagus for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    hM3Dq ( partial ) (CHRM3 Human)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP-Nsp15

    Plasmid

    #165120

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp15 (ORF1ab codon optimized SARS-COV-2)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLX_TRC311 ETV4-S

    Plasmid

    #74983

    Purpose

    Constitutive ETV4 ORF, blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV4 (ETV4 )
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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