162,408 results

1. SPLICS LY-MT Short P2A

   Plasmid

   #213614

   Purpose

   Detect the short-range Lysosome-Mitochondria contact

   Depositor

   Tito Calì

   Article

   Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

   Insert

   SPLICS LY-MT Short P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2,0_3M5M_eGFP

   Plasmid

   #135474

   Purpose

   Encodes Marburg virus minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoter

   Depositor

   Elke Mühlberger

   Article

   Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

   Insert

   Marburg virus minigenome, eGFP reporter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   T7

   Available since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. 35S-GWY-GAL4BD/pAM

   Plasmid

   #201231

   Purpose

   Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

   Depositor

   Michael Ayliffe

   Article

   Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

   Insert

   35S-GWY-GAL4BD
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. RCASBP-Y DV

   Plasmid

   #11478

   Depositor

   William Pavan

   Article

   Loftus et al DNA Res. 2001 Oct 31. 8(5):221-6.

   Insert

   Gateway ccdB reading frame cassette
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pORANGE GFP-Grin1 KI

   Plasmid

   #131485

   Purpose

   Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Grin1, Grin1 Mouse, Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Flag-IDH1

   Plasmid

   #62906

   Purpose

   Expresses wildtype IDH1 in mammalian cells

   Depositor

   Yue Xiong

   Article

   Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

   Insert

   isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-Rab5DN(S34N)

   Plasmid

   #35139

   Depositor

   Sergio Grinstein

   Article

   Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

   Insert

   Rab5a (RAB5A Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Dominant Negative- S34N

   Promoter

   CMV

   Available since

   March 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tad-CBE(CCG3)

   Plasmid

   #235074

   Purpose

   Expresses Tad-CBE(CCG3) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(CCG3)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. GRM1-DuET

   Plasmid

   #213300

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GRM1 (GRM1 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. EFSp-GFP-YAP (5SA)

    Plasmid

    #174170

    Purpose

    Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the EFS promoter.

    Depositor

    Rod Bremner

    Article

    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

    Insert

    YAP (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    3x FLAG tag

    Expression

    Mammalian

    Mutation

    Five LATS phosphorylation sites (S61, S109, S127,…

    Promoter

    EFS

    Available since

    Sept. 28, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40

    Plasmid

    #105545

    Purpose

    AAV expression of EGFP-Cre from CMV promoter

    Depositor

    James M. Wilson

    Has Service

    AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP-Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HI

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-SFPQ

    Plasmid

    #166959

    Purpose

    Expresses HA-tagged SFPQ protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ (SFPQ Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Piggybac-TetO-hNurr1-blast

    Plasmid

    #176485

    Purpose

    TetO-hNurr1 in a PiggyBac vector with Blasticidin resistance

    Depositor

    Marius Wernig

    Article

    Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

    Insert

    Nurr1 (NR4A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. NLS-SpCas9mSA-His

    Plasmid

    #182951

    Purpose

    Express SpCas9mSA in bacteria

    Depositor

    Wen Xue

    Article

    Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

    Insert

    NLS-SpCas9mSA-His
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOPINK-OTUD3 (OTU, aa 52-209)

    Plasmid

    #61412

    Purpose

    Expresses human OTUD3 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD3 (OTUD3 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-51 and aa 210-398.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET23b-Casp3 C163A-His

    Plasmid

    #11822

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 3 catalytic mutant (CASP3 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    C163A

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-hSyn-GRAB-rDA3m

    Plasmid

    #208702

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neurons

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Has Service

    AAV1

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA3m
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. V51 pIRESpuro-GLUE (pGLUE)

    Plasmid

    #15100

    Purpose

    tandem-affinity purification plasmid; streptavidin-(streptag) and calmodulin-(CBP) binding affinity tags

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Type

    Empty backbone

    Use

    Tags

    CBP tag, HA, and streptag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. TFORF1035

    Plasmid

    #143746

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    WT1 (WT1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TFORF1038

    Plasmid

    #144075

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    WT1 (WT1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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