159,557 results

1. TFORF3131

   Plasmid

   #144607

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NR5A2 (NR5A2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDD427

   Plasmid

   #202080

   Purpose

   Frame +2/-1 selector for NHEJ knock-in

   Depositor

   Daniel Dickinson

   Article

   Shi et al Development. 2023 May 15;150(10):dev2013

   Insert

   pEF1a-mCherry-Cas9 + Frame +2/-1 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. DYRK1A_pCSdest

   Plasmid

   #53791

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   DYRK1A (DYRK1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   Sept. 8, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pBiFC-PBZ-4A-VC

   Plasmid

   #110649

   Purpose

   expresses a PAR-binding (PBZ) domain fused to venus C-terminus, contains point mutations that abolish PAR binding

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

   Insert

   PBZ domain from APLF, amino acids 371-451 (APLF Human)
   

   Use

   Tags

   HA and VC

   Expression

   Mammalian

   Mutation

   C379A, C385A, C421A and C427A

   Promoter

   CMV

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tom20-CR

   Plasmid

   #171461

   Purpose

   Expresses Tom20-CR, an anchor protein of iCMM/NiCMM, in mammalian cells

   Depositor

   Takafumi Miyamoto

   Article

   Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

   Insert

   Tom20 (1-34 aa)-ECFP-FRB (2021-2113 aa)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. V5-GFP-APEX2-NES_pLX208

   Plasmid

   #202018

   Purpose

   expresses GFP-APEX2 in the mammalian cytosol, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   V5-GFP-APEX2-NES
   

   Use

   Lentiviral

   Tags

   GFP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDZ2FE_Pogz_1-1410

   Plasmid

   #105674

   Purpose

   Expresses Flag-tagged full-length human POGZ protein

   Depositor

   Thanos Halazonetis

   Insert

   POGZ (POGZ Human)
   

   Use

   Tags

   2X Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-TREtight-mTagBFP2-B19G

   Plasmid

   #100799

   Purpose

   helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

   Depositor

   Ian Wickersham

   Article

   Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

   Has Service

   AAV1

   Insert

   mTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TREtight

   Available since

   Oct. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEXP(V5_H2B_APEX2)

   Plasmid

   #107597

   Purpose

   Expresses V5_H2B_APEX2 in mammallian cells

   Depositor

   Markus Hafner

   Article

   Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

   Insert

   H2B-APEX2
   

   Use

   Tags

   H2B and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Dox inducible

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. EBV BGLF4

    Plasmid

    #26691

    Depositor

    Robert Kalejta

    Article

    Kuny et al PLoS Pathog. 2010 . 6(9):.

    Insert

    BGLF4
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pJT119b

    Plasmid

    #50551

    Purpose

    Expresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoter

    Depositor

    Jeffrey Tabor

    Article

    Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

    Inserts

    CcaS
    CcaR
    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pInducer-myc-ERK1-R84S/S170D

    Plasmid

    #213593

    Purpose

    Doxycycline inducible expression of myc-tagged ERK1-R84S/S170D cDNA in mammilian cells

    Depositor

    Channing Der

    Article

    Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

    Insert

    MAPK3 (MAPK3 Human)
    

    Use

    Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    R84S, S170D

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. mRFP-Rab5

    Plasmid

    #14437

    Purpose

    Mammalian expression of Rab5 fused to mRFP. Used for localization to the early endosome, and other early endosomal vacuoles.

    Depositor

    Ari Helenius

    Article

    Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

    Insert

    Rab 5 (RAB5A Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJH373

    Plasmid

    #86840

    Purpose

    mamalian expression of anti-CRISPR protein AcrIIA2

    Depositor

    Joseph Bondy-Denomy

    Article

    Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

    Insert

    acrIIA2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. enz.099-PKA

    Plasmid

    #154048

    Purpose

    Expresses PKA in E. coli

    Depositor

    Christopher Voigt

    Article

    Wallace et al Advanced Functional Materials 2020

    Insert

    Protein kinase cAMP-activated catalytic subunit alpha (PKA) (PRKACA Human)
    

    Use

    Tags

    His, MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-N1-1x

    Plasmid

    #172281

    Purpose

    Expressing EGFP mRNA fused with 1 tandem repeat of a 50-base sequence

    Depositor

    Antony K Chen

    Article

    Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

    Insert

    EGFP-N1-1x
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. H11-mScarletSIIN

    Plasmid

    #172438

    Purpose

    Hipp11 targeting vector, expresses mScarlet-SIINFEKL

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Insert

    mScarlet-SIIN
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pORTMAGE311B

    Plasmid

    #120418

    Purpose

    Expresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanR

    Depositor

    Csaba Pál

    Article

    Szili et al bioRxiv (2018)

    Inserts

    MutL E32K
    Lambda Beta
    XylS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E32K mutation conferring dominant mutator phenoty…

    Promoter

    Pm

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTP955

    Plasmid

    #104164

    Purpose

    Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (3x Cysteines)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-rabbit IgG Fc specific nanobody TP897 (3xCysteines)
    

    Use

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-ND1 Left -GSVG-UGI

    Plasmid

    #187412

    Purpose

    human mitochondrial DNA ND1 target TALE

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA GSVG variant
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge