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19,819 results

Showing: 7521 - 7540 of 19819 results

  2. pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)

    Plasmid
    #103866
    Purpose
    dPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GSGGGGS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103866-plasmid-map-sequence-id-199147

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  5. pCS2 GFP-GSK3mut-MAPK

    Plasmid
    #29690
    Purpose
    GFP reporter protein fused to three mutated GSK3 phosphorylation sites
    Depositor
    Edward De Robertis
    Article
    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.
    Insert
    GFP glycogen synthase kinase 3 recognition site mutated primed by MAPK
    Tags
    Flag and Flag-EGFP
    Expression
    Mammalian
    Mutation
    recognition sites only, and E3 ligase site, GSK3 …
    Promoter
    SP6
    Available Since
    Jan. 6, 2012
    Availability
    Academic Institutions and Nonprofits only
    29690-plasmid-map-sequence-id-129831

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  9. lentiCRISPR - EGFP sgRNA 1

    Plasmid
    #51760
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available Since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51760-plasmid-map-sequence-id-392672

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  15. pMito-TrxRFP1

    Plasmid
    #98997
    Purpose
    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, mitochondrially locaclized
    Depositor
    Huiwang Ai
    Article
    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038
    Insert
    TrxRFP1 and mitochondrial localization sequence
    Tags
    His-tag and Mitochondria localization sequence
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    98997-plasmid-map-sequence-id-189692

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  20. tol2-mpeg1-mcherry

    Plasmid
    #58935
    Purpose
    labels macrophages with mcherry
    Depositor
    Anna Huttenlocher
    Insert
    mpeg1-mcherry
    Promoter
    mpeg1
    Available Since
    Sept. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    58935-plasmid-map-sequence-id-91295

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Showing: 7521 - 7540 of 19819 results