19,660 results

1. pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag

   Plasmid

   #132400

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPguCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H151A; H1121A

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-PS11-scFvFc-CD28-gp41 (706-713)

   Plasmid

   #60606

   Purpose

   Mammalian Display vector

   Depositor

   Wayne Marasco

   Article

   Taube et al PLoS One. 2008 Sep 11;3(9):e3181. doi: 1

   Insert

   PS11-scFvFc-CD28-gp41 (706-713) (CD28 Human)
   

   Tags

   C9

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pC019 - LwCas13a from Leptotrichia wadei

   Plasmid

   #91909

   Purpose

   Expresses LwCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LwCas13a and guide expression cassette
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 4, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pBG100-TCPTP

   Plasmid

   #33365

   Depositor

   Ambra Pozzi

   Article

   Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

   Insert

   TCPTP-45 (PTPN2 Human)
   

   Tags

   6xHIS - cleavable

   Expression

   Bacterial

   Promoter

   T7lac

   Available Since

   Dec. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pRSFDuet-sumo-h-cGAS

   Plasmid

   #127161

   Purpose

   expression of human cGAS protein

   Depositor

   Thomas Tuschl

   Article

   Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

   Insert

   MB21D1 (CGAS Human)
   

   Tags

   His-SUMO tag

   Expression

   Bacterial

   Mutation

   None

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-WT-STAT1

   Plasmid

   #71454

   Purpose

   Lentiviral expression of WT-STAT1

   Depositor

   George Stark

   Article

   Cheon et al Proc Natl Acad Sci U S A. 2009 Jun 9;106

   Insert

   STAT1 (STAT1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Promoter

   CMV

   Available Since

   Dec. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1-V5-PTK2B

   Plasmid

   #127233

   Purpose

   Eukaryotic expression of PTK2B

   Depositor

   Kai Johnsson

   Article

   Haruki et al PLoS One. 2012;7(5):e37598. doi: 10.1371

   Insert

   PTK2B
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. p6XHis_NLS-SaCas9

   Plasmid

   #101086

   Purpose

   Express SaCas9 in bacteria with a 6xHis tag for purification

   Depositor

   Rick Tarleton

   Article

   Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

   Insert

   CRISPR-associated protein Cas9/Csn1
   

   Use

   CRISPR

   Tags

   TrxA-6xHis-STag

   Expression

   Bacterial

   Promoter

   T7lac

   Available Since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSBtet-Bla

   Plasmid

   #60510

   Purpose

   SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and blasticidin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Expression

   Mammalian

   Promoter

   TCE & RPBSA

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-D3cpv

    Plasmid

    #36323

    Depositor

    Amy Palmer, Roger Tsien

    Article

    Palmer et al Chem Biol. 2006 May;13(5):521-30.

    Insert

    D3cpv second generation cameleon (calcium sensor)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. Col1a-frt-hygro-pA

    Plasmid

    #20730

    Depositor

    Rudolf Jaenisch

    Article

    Beard et al Genesis. 2006 Jan . 44(1):23-8.

    Insert

    Col1A-frt-hygro-pA
    

    Use

    Mouse Targeting

    Available Since

    April 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. dCas9-CBP FL

    Plasmid

    #179550

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-GFAP-hM4D(Gi)-mCherry

    Plasmid

    #50479

    Purpose

    Gi-coupled hM4D DREADD fused with mCherry under the control of GFAP promoter

    Depositor

    Bryan Roth

    Has Service

    AAV5

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    GFAP

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. Dazl-CPG-TV

    Plasmid

    #70144

    Purpose

    a targeting vector, to target the endogenous DAZL CPG island with Snrp-GFP-polyA

    Depositor

    Rudolf Jaenisch

    Article

    Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

    Insert

    5'hDAZL-snrp-GFP-polyA-pgK-PURO-3'hDAZL
    

    Use

    Mouse Targeting and Synthetic Biology

    Available Since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLCAG EGFP

    Plasmid

    #14857

    Depositor

    Inder Verma

    Article

    Pfeifer et al Proc Natl Acad Sci U S A. 2002 Feb 19. 9

    Insert

    EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 11, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pRsetB-PercevalHR

    Plasmid

    #49081

    Purpose

    Bacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

    Depositor

    Gary Yellen

    Article

    Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

    Insert

    PercevalHR
    

    Tags

    7xHis

    Expression

    Bacterial

    Available Since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. PSD-95-pTagRFP

    Plasmid

    #52671

    Purpose

    expression of TagRFP-tagged PSD-95

    Depositor

    Johannes Hell

    Insert

    PSD-95 (Dlg4 Rat)
    

    Tags

    tagRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.2 TDP-43 NLS1, 5F-L YFP

    Plasmid

    #84913

    Purpose

    Mammalian expresion of TDP-43 NLS1, 5F-L YFP

    Depositor

    Aaron Gitler

    Article

    Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    K82A, R83A, K84A, F147L, F149L, F194L, F229L, F23…

    Promoter

    CMV

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNLS-iRFP713

    Plasmid

    #45468

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP713
    

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104T, V114I, E161K, E193K, F198…

    Promoter

    CMV

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMCL-HA-MAPKK1-R4F [delta(31-51)/S218E/S222D]

    Plasmid

    #40810

    Depositor

    Natalie Ahn

    Article

    Mansour et al Science. 1994 Aug 12;265(5174):966-70.

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta(31-51), S218E, S222D

    Promoter

    CMV

    Available Since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only