We narrowed to 13,006 results for: Hal

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pDRf1-4CL5-AtSCT (JBEI-11557)

Plasmid

#87937

Purpose

Co-expression in S.cerevisiae of 4CL5 and AtSCT

Depositor

Dominique Loque

Article

Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

Inserts

AtSCT
4CL5 (4CL5 Mustard Weed)

Expression

Yeast

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV Luc2 (FF-Luciferase) L5-L2

Plasmid

#62168

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and firefly luciferase module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

Luciferase

Use

Gateway Cloning and Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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AKT-APEX2-P2A-mKate2

Plasmid

#67654

Purpose

Mammalian expression vector driving AKT-APEX2 (Human AKT-PH domain, [marks PI(3,4)P2 and PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

AKT-PH-APEX2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-DIO-erHRP(N175S mutant)

Plasmid

#79906

Purpose

Adeno-associated virus plasmid, Cre-dependent FLEX switch, erHRP(N175S mutant)

Depositor

Joshua Sanes

Article

Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

Insert

erHRP(N175S mutant)

Use

AAV and Cre/Lox

Tags

HA tag

Expression

Mammalian

Mutation

N175S mutation

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pOPINK-VCPIP (OTU, aa 25-561)

Plasmid

#61583

Purpose

Expresses human VCPIP (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

VCPIP (VCPIP1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-24 and aa 562-1222.

Promoter

T7

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jip1 JBD

Plasmid

#15751

Purpose

binds to Jnk

Depositor

Roger Davis

Article

Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

Insert

Jip1 (Jnk binding domain) (Mapk8ip1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

deleted 1-126 and 282-660

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only
CFP-GAI(1-532)

Plasmid

#37310

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Tags

CFP

Expression

Mammalian

Mutation

contains aa 1-532

Promoter

CMV

Available Since

June 15, 2012

Availability

Academic Institutions and Nonprofits only
pORANGE WASF1-GFP KI

Plasmid

#131502

Purpose

Endogenous tagging of WASP1/WAVE1: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Wasf1 Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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CRY2-mCherry-iTrkA

Plasmid

#106168

Purpose

mammalian expression of CRY2PHR (aa 1-498) fused to mCherry and intracellular domain of TrkA (aa 450-799)

Depositor

Bianxiao Cui

Article

Duan et al ACS Synth Biol. 2018 Jul 20;7(7):1685-16

Insert

CRY2PHR mCherry iTrkA (CRY2 Mustard Weed, Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-PAZ(aa1-480)

Plasmid

#21529

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago2 (AGO2 Human)

Tags

3XFLAG

Expression

Mammalian

Mutation

contain only aa1-480

Available Since

July 29, 2009

Availability

Academic Institutions and Nonprofits only
CoV2-N-D3L

Plasmid

#177945

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (D3L) (N )

Expression

Mammalian

Mutation

D3L

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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BTK-APEX2-P2A-mKate2

Plasmid

#67653

Purpose

Mammalian expression vector driving BTK-APEX2 (Human BTK-PH domain, [marks PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

BTK-PH-APEX2

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD1 (OTU, aa 287-437)

Plasmid

#61407

Purpose

Expresses human OTUD1 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD1 (OTUD1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-286 and aa 438-481.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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CoV2-N-A119S

Plasmid

#177948

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (A119S). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (A119S) (N )

Expression

Mammalian

Mutation

A119S

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-β-arrestin2 RRK/Q

Plasmid

#38261

Depositor

Robert Lefkowitz

Article

Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

Insert

β-arrestin2 (Arrb2 Rat)

Tags

Flag

Expression

Mammalian

Mutation

Arg233, Arg237, and Lys251 converted to Glutamines

Promoter

CMV

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
pSL0373 (His-MBP-TniQ)

Plasmid

#130638

Purpose

Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite tag for purification.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTniQ

Use

CRISPR; Transposon

Tags

His10-MBP-TEVsite on TniQ

Expression

Bacterial

Promoter

T7

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV CreERT2 R4-R3

Plasmid

#62173

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and CreERT2 module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

CreERT2

Use

Gateway Cloning

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMDC32B-AtMIR390a-NbSu-2

Plasmid

#213400

Purpose

Plant expression vector (2x35S) for expressing an amiRNA against Nicotiana benthamiana SULFUR gene from AtMIR390a precursor

Depositor

Alberto Carbonell

Article

Cisneros et al Plant J. 2022 May;110(4):1166-1181. doi:

Insert

Arabidopsis MIR390a precursor including amiRNA/amiRNA* sequences for silencing N. benthamiana SULFUR gene

Expression

Plant

Promoter

2x35S

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pJL1-mRFP1 gRNA

Plasmid

#117052

Purpose

In vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

Insert

anti-mRFP1 SpCas9 gRNA

Expression

Bacterial

Promoter

T7

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUB2 (full-length, aa 1-234)

Plasmid

#61421

Purpose

Expresses human OTUB2 (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUB2 (Ubiquitin thioesterase OTUB2) (OTUB2 Human)

Tags

His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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