We narrowed to 9,406 results for: pho;

1. pET29-hMIGA2(170-593)-6xhis

   Plasmid

   #192834

   Purpose

   Express codon-optimized human MIGA2 fragment 170-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

   Depositor

   Karin Reinisch

   Article

   Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

   Insert

   Codon-optimized human Mitoguardin 2 (MIGA2 Human)
   

   Tags

   6xhis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMY-CD3-P2A-CD90.2

   Plasmid

   #163338

   Purpose

   Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the LTR promoter

   Depositor

   Louis Ates, Teunis Geijtenbeek

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Insert

   CD3
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMX-CAG-CD3-P2A-CD90.2

   Plasmid

   #163336

   Purpose

   Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the CAG promoter

   Depositor

   Louis Ates, Teunis Geijtenbeek

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Insert

   CD3
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRX-IP-hWIPI4-3×FLAG

   Plasmid

   #215512

   Purpose

   Expresses FLAG tagged human WIPI4.

   Depositor

   Noboru Mizushima

   Article

   Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

   Insert

   WD-repeat protein interacting with phosphoinositides 4 (WDR45 Human)
   

   Use

   Retroviral

   Tags

   3×FLAG

   Expression

   Mammalian

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. U6-hGRIN2B-CAG-ps-SaCas9

   Plasmid

   #102853

   Purpose

   A single-chain light-controllable dSaCas9 with pdDronpa1 domains for hGRIN2B gene editing

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   sa-hGRIN2B-sgRNA; dSaCas9; pdDronpa1 (GRIN2B S. aureus, Synthetic, Human)
   

   Use

   CRISPR

   Tags

   3X Flag and NLS

   Expression

   Mammalian

   Promoter

   U6 promoter;

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV hSyn mCh-IRES-BoNT/B(1-146)-iLID

   Plasmid

   #122982

   Purpose

   AAV plasmid with human synapsin promoter driving mCh and BoNT/B amino acids 1-146 fused to iLID(V416I), separated with an IRES element. Co-express with SSPB-BoNT(147-441, Y365A) for sPA-BoNT

   Depositor

   Matthew Kennedy

   Article

   Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

   Insert

   mCh-IRES-BoNT/B(1-146)-iLID
   

   Use

   AAV

   Tags

   mCh

   Expression

   Mammalian

   Mutation

   iLID contains long lived V416I mutation

   Promoter

   human synapsin

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A

   Plasmid

   #22023

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   mVenus-PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   The LOV domain contains the mutation C450A.Rac1 s…

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-mCherry-TSC2(AA,3Q)-NLS

   Plasmid

   #192445

   Purpose

   Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity; targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q )-NLS (TSC2 Human)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-mCherry.3xFLAG.NOS1AP-S-WPRE

   Plasmid

   #127867

   Purpose

   pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP-S fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   Nos1ap (Nos1ap Mouse, Human)
   

   Use

   AAV

   Tags

   3xFLAG and mCherry

   Mutation

   constructed by blunt end fusion of the 5’ 51 nucl…

   Promoter

   hSyn

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-OptoFGFR1-Y766F

    Plasmid

    #59777

    Purpose

    Expresses optoFGFR1 with mutation in PLC-gamma binding phosphotyrosine site of FGFR1

    Depositor

    Won Do Heo

    Article

    Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

    Insert

    OptoFGFR1-Y766F (FGFR1 Mustard Weed, Human)
    

    Tags

    mCitrine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBT270_(pCA-G-intron(Neo)-tTA2-iiTRE-tdT3Mycii)

    Plasmid

    #36881

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tTA2
    beta-globin intron
    Neo
    tdT-3Myc
    insulator
    

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available Since

    Aug. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRIP-SFFV-mtagBFP-2A STINGHAQ V155M

    Plasmid

    #102592

    Purpose

    Lentivector to express STING V155M HAQ and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    V155M R71H-G230A-R293Q

    Promoter

    SFFV

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. LENTICRISPR-HNRNPD_sgRNA1

    Plasmid

    #201598

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    HNRNPD (HNRNPD Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBabe-TetCMV-puro-mPA-GFP-PA-Rac1

    Plasmid

    #22032

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mPA-GFP-PA-Rac1 (RAC1 Avena sativa (oat), Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA6-N-3XFLAG-Gp78

    Plasmid

    #123590

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    autocrine motility factor receptor (Amfr Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. LENTICRISPR-PABPC1_sgRNA1

    Plasmid

    #201608

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    PABPC1 (PABPC1 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP Kind2 S159/181/666A

    Plasmid

    #105310

    Purpose

    To study altered downstream functions of phospholylation-site null kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S159/181/666A

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET29-hMIGA2(306-593)-6xhis

    Plasmid

    #192837

    Purpose

    Express codon-optimized human MIGA2 fragment 306-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    

    Tags

    6xhis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR1A-PIK3CA E545K

    Plasmid

    #180032

    Purpose

    Entry vector of PIK3CA E545K for gateway cloning.

    Depositor

    Xin Chen

    Article

    Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv

    Insert

    PIK3CA E545K (PIK3CA Human)
    

    Use

    Entry vector

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    E545K

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-EF1a-Ly6G-P2A-PuroR-HA-miR30

    Plasmid

    #163359

    Purpose

    Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a Ly6G surface marker and a puromycin selection marker.

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    Ly-6G (Ly6g Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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