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pRU321

Plasmid

#167681

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUB4-2::asCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU320

Plasmid

#167682

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::asCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer-TAZ-S89A

Plasmid

#213588

Purpose

Doxycycline inducible expression of TAZ-S89A cDNA in mammalian cells

Depositor

Channing Der

Article

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

TAZ (WWTR1 Human)

Use

Lentiviral

Tags

2xHA

Expression

Mammalian

Mutation

S89A

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST26-NAT1-C-HA

Plasmid

#195178

Purpose

Vector for transient expression of NAT1 gene with C-terminal HA tag, gene flanked by att sites for gateway cloning

Depositor

Stephanie Roessler

Insert

NAT1 (NAT1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV promoter

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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MTOR-F2108L_CD19-CAR-28z-2A-EGFP_AAV6_Donor

Plasmid

#211904

Purpose

Vector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.

Depositor

Yannick Doyon

Article

Levesque et al bioRxiv 2023.09.14.557485

Insert

CD19-CAR-28z-2A-EGFP

Use

AAV

Expression

Mammalian

Promoter

hPGK1

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Bet1-mCherry

Plasmid

#209848

Purpose

Expresses Bet1-mCherry in mammalian cells

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

Bet1 (BET1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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Bet1-GFP

Plasmid

#209850

Purpose

Expresses Bet1-GFP in mammalian cells

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

Bet1 (BET1 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-Lyn11C32S-PIP4K2A

Plasmid

#202748

Purpose

weakly plasma membrane targeted fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:LYN (1-11) (C3S):PIP4K2A (PIP4K2A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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PDGFRalpha-D842V #1

Plasmid

#201407

Purpose

Inserts PDGFRalpha-D842V to activate the MAP kinase pathway.

Depositor

Maria Castro

Article

Carney et al Clin Cancer Res. 2023 Jan 24:CCR-22-1896

Insert

PDGFRalpha-D842V (Pdgfra Mouse)

Expression

Mammalian

Mutation

D842V

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRCP

Plasmid

#195862

Purpose

Encodes chromogenic protein (CP). CP inducible with rhamnose.

Depositor

Gregory Phillips

Article

DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

Insert

Chromogenic Protein

Expression

Bacterial

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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DDX11

Plasmid

#155502

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX11 (DDX11 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PPAPDC2

Plasmid

#155836

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PPAPDC2 (PLPP6 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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RAT

Plasmid

#187416

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1 (Atp1a1 Rat)
ATP1B1 (Atp1b1 Rat)

Expression

Insect

Promoter

PH and p10

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBlueScript II SK-SNAP donor for H3f3b

Plasmid

#186933

Purpose

Genomic targeting of SNAP tag at H3f3b C-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

SNAP donor for H3f3b (H3f3b Mouse)

Tags

SNAP tag

Expression

Mammalian

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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C204

Plasmid

#174358

Purpose

For expressing siRNA-resistant human PABPC1 in human cell lines via transfection

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

PABPC1 (PABPC1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Multiple silent mutations introduced to be resist…

Available Since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pT7C-HSPA1L

Plasmid

#177660

Purpose

Recombinant protein synthesis of human HSPA1L

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

HSPA1L (HSPA1L Human)

Tags

6xHis-Thrombin

Expression

Bacterial

Promoter

T7

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-AMBRA1-3xFLAG

Plasmid

#174159

Purpose

PiggyBac vector to stably express 3xFLAG-tagged AMBRA1

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA1 (AMBRA1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

EF1A

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pETM10 with rat FL Annexin A11

Plasmid

#164496

Purpose

For bacterial expression of rat His-tagged FL Annexin A11

Depositor

Anni Vedeler

Article

Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

Insert

rat FL Annexin A11 (Anxa11 Rat)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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SnFR-gamma2

Plasmid

#165495

Purpose

Green fluorescent glutamate reporter fused to TARP gamma-2 for mammalian expression.

Depositor

Andrew Plested

Article

Hao et al Elife. 2023 Jan 9;12:e84029. doi: 10.755

Insert

iGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Synthetic, Rat)

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX/PD-L1 3SA

Plasmid

#121467

Purpose

Expression of GST-human PD-L1 in E. Coli

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Tags

GST

Expression

Bacterial

Mutation

S176A, T180A, S184A

Promoter

Tac

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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