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Plasmid EUPp_09

Plasmid

#157745

Purpose

PgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)

Depositor

Kang Zhou

Article

Liang et al Metab Eng. 2020 Nov 25. pii: S1096-7176(

Inserts

acetaldehyde dehydrogenase
alcohol dehydrogenase (ADH2 Budding Yeast)

Expression

Bacterial

Promoter

PgyrA

Available Since

June 4, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-EN1201

Plasmid

#92144

Purpose

For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus

Expression

Mammalian

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-LifeAct-GFP

Plasmid

#190199

Purpose

Expresses the small actin binding peptide, LifeAct, with a GFP reporter

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

LifeAct-eGFP

Use

AAV

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQAT28

Plasmid

#223400

Purpose

T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and sgRNA was driven by OsU3; Hygromycin for plant select.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP2

Plasmid

#25031

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DCP2 (DCP2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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IgK-IFN-luc

Plasmid

#14886

Purpose

Reporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expression

Depositor

David Baltimore

Article

Pomerantz et al EMBO J. 2002 Oct 1. 21(19):5184-94.

Insert

IgK-IFN (IFNB1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

May 1, 2007

Availability

Academic Institutions and Nonprofits only

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pENTR2b-mScarlet

Plasmid

#207961

Purpose

Gateway entry vector with the mScarlet fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

mScarlet

Use

Gateway entry vector

Promoter

None

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pC003 - LshC2C2 locus into pACYC184 for spacer cloning

Plasmid

#79152

Purpose

LshC2c2 locus in pACYC184 with BsaI sites for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

LshC2c2 locus

Mutation

*

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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PZac2.1 gfaABC1D-Aqp4-eGFP

Plasmid

#176747

Purpose

Expresses Aquaporin-4 fused with eGFP at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Aqp4-eGFP

Use

AAV

Tags

GFP

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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C3 luc (T1 del)

Plasmid

#11358

Depositor

Donald McDonnell

Article

Fan et al Mol Endocrinol. 1996 Dec . 10(12):1605-1

Insert

C3 promoter (C3 Human)

Use

Luciferase

Expression

Mammalian

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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pLentiCas9-GFP

Plasmid

#78546

Purpose

Fusion protein Cas9+GFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Promoter

Fusion protein with Cas9

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pBT372

Plasmid

#125613

Purpose

Expresses evoCDA1-BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoCDA1-BE4max-NG

Expression

Mammalian

Mutation

F23S A123V I195F

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pPerimCh

Plasmid

#227013

Purpose

Expresses periplasmic mCherry

Depositor

Suzan Rooijakkers

Article

Heesterbeek et al Sci Rep. 2019 Feb 28;9(1):3074. doi: 10.

Insert

PelB-mCherry, arabinose inducible cytosolic GFP

Expression

Bacterial

Promoter

rpsM and pBAD

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCIG3 (pCMV-IRES-GFP version 3)

Plasmid

#78264

Purpose

Lentiviral empty cloning vector containing IRES and GFP

Depositor

Felicia Goodrum

Article

Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CMV, lentiviral LTR

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp9 C287A

Plasmid

#11819

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

Insert

Caspase 9 catalytically incompetent mutant (CASP9 Human)

Expression

Mammalian

Mutation

C287A, catalytically incompetent mutant.

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

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pLentiCas9-T2A-GFP

Plasmid

#78548

Purpose

Cas9 protein with GFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

T2A-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

In frame with Cas9

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pLIB-HIS-TEV-scCAK1

Plasmid

#177014

Purpose

Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)

Tags

Polyhistidine-TEV

Expression

Insect

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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ARL13B-(ΔGTPase)- GFP

Plasmid

#246843

Purpose

Express Arl13B (resistance to sgRNA) with deletion of GTPase, tagged with GFP

Depositor

Lei Lu

Article

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

deleted GTPase domain

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAcCas12nHS_RV6_empty

Plasmid

#203811

Purpose

Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12nHS_RV6

Use

CRISPR

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGoldenGreenGate-M (pGGG-M)

Plasmid

#165422

Purpose

A Golden Gate (MoClo) compatible Level 2 acceptor binary vector based on pGreenII (Hellens et al., 2000).

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Article

Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Level 2 golden gate (moclo) du…

Promoter

Contain GG cassette and MCS with LacZ for blue/wh…

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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