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pDest_U6_A2_CG2_T2_gRNA

Plasmid

#199388

Purpose

pDEL112; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type II sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

nrg1 type II sgRNA

Use

Tol2 destination vector

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_T3_gRNA

Plasmid

#199389

Purpose

pDEL113; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type III sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

nrg1 type III sgRNA

Use

Tol2 destination vector

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_typeEGF_gRNA

Plasmid

#199386

Purpose

pDEL110; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 EGF sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

nrg1 EGF sgRNA

Use

Tol2 destination vector

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-ShadowY

Plasmid

#193555

Purpose

Expresses mEGFP-ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

ShadowY

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-Scarlet-I

Plasmid

#193558

Purpose

Expresses mTurquoise2-mScarlet-I in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

mTurquoise2-mScarlet-I

Expression

Mammalian

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCIS-rPAM3

Plasmid

#145803

Purpose

expresses rat PAM3 in mammalian cells

Depositor

Betty Eipper, Richard Mains

Article

Milgram et al J Cell Biol. 1994 Jan;124(1-2):33-41. do

Insert

rat PAM3

Expression

Mammalian

Mutation

See depositor comments below

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pQuantA-hygro-kana

Plasmid

#45585

Depositor

Andrzej Dziembowski

Article

Orlowska et al Nucleic Acids Res. 2013 Sep;41(17):e167.

Type

Empty backbone

Use

Cre/Lox; Dt40 cell line targeting

Tags

Quant-A tag

Available Since

Oct. 3, 2013

Availability

Academic Institutions and Nonprofits only

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Doughnut (DNT)

Plasmid

#17580

Depositor

Denise Montell

Article

Savant-Bhonsale et al Gene. 1999 Apr 29. 231(1-2):155-61.

Insert

dnt (dnt Fly)

Expression

Bacterial

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pSKB3.MPN314

Plasmid

#11433

Depositor

Sung-Hou Kim

Article

Chen et al Proteins. 2004 Jun 1. 55(4):785-91.

Insert

cell division protein

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pPB-CAG-rtTA3G-IH

Plasmid

#214118

Purpose

Gene expression by Piggybac transposase in mammalian cells

Depositor

Sho Yoshimatsu

Insert

trTA3G

Expression

Mammalian

Mutation

Codon-optimized for mammalian cells

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-rtTAM2-IB

Plasmid

#214117

Purpose

Gene expression by Piggybac transposase in mammalian cells

Depositor

Sho Yoshimatsu

Insert

rtTAM2

Expression

Mammalian

Mutation

Codon-optimized for mammalian cells

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCSC-NGN2-IRES-ISL1-T2A-LHX3

Plasmid

#221814

Purpose

Lentiviral vector expressing human NEUROG2, ISL1 and LHX3 for inducing pluripotent stem cells into cholinergic motor neurons.

Depositor

Baojin Ding

Article

Sepehrimanesh et al Am J Physiol Cell Physiol. 2020 Oct 1;31

Inserts

NEUROG2 (NEUROG2 Human)
ISL1 (ISL1 Human)
LHX3 (LHX3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-hSOX2-17

Plasmid

#193350

Purpose

Retroviral vector expressing human SOX2-17 chimeric transcription factor (super-SOX)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2-17 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

SOX2-17 is engineered highly cooperative chimeric…

Promoter

5'LTR

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-Sox2-17

Plasmid

#193353

Purpose

Retroviral vector expressing mouse Sox2-17 chimeric transcription factor (super-Sox)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Sox2-17 (Sox2 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

Sox2-17 is engineered highly cooperative chimeric…

Promoter

5'LTR

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-OSK

Plasmid

#136552

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4-P2A-Sox2-T2A-Klf4 (Klf4, Sox2, Pou5f1 Mouse)

Use

Lentiviral

Expression

Bacterial

Promoter

tetO-miniCMV (dox-inducible)

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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peGFP-N1-Cofilin

Plasmid

#186747

Purpose

Expresses wildtype GFP-tagged human cofilin in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Cofilin-1 (CFL1 Human)

Tags

GFP

Expression

Mammalian

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCXLE-SOX2-p2a-KLF4

Plasmid

#193292

Purpose

episomal hSK (expressing human SOX2 + KLF4)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2-p2a-KLF4 (SOX2, KLF4 Human)

Expression

Mammalian

Mutation

F2A of original pCXLE-SK vector was replaced with…

Promoter

CAG

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pmCherry-N1-cofilin

Plasmid

#186750

Purpose

Expresses wildtype mCherry-tagged human cofilin in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Cofilin-1 (CFL1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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peGFP-N1-Cofilin-S41E

Plasmid

#186748

Purpose

Expresses S41E phosphomimetic GFP-tagged human cofilin in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Cofilin-1 (CFL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed serine 41 to glutamic acid

Promoter

CMV

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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peGFP-N1-Cofilin-S41A

Plasmid

#186749

Purpose

Expresses S41A phosphodead GFP-tagged human cofilin in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Cofilin-1 (CFL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed serine 41 to alanine

Promoter

CMV

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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