We narrowed to 19,092 results for: iNOS

1. pXPR_023d-sgCiSF3B1

   Plasmid

   #202446

   Purpose

   Expression of a CRISPRi guide targeting SF3B1

   Depositor

   William Hahn

   Article

   Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

   Insert

   SF3B1 gRNA (SF3B1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Pvalb-2A-Cre targeting vector

   Plasmid

   #61570

   Purpose

   Target the Cre recombinase gene to the stop codon of the mouse Pvalb gene

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   Pvalb exon 4 - 2A - Cre
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Pvalb-2A-Flpo Flp-in replacement vector

   Plasmid

   #61572

   Purpose

   Recombinase-mediated cassette exchange in ES cells to insert the Flpo recombinase gene at the mouse Pvalb gene stop codon

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   Pvalb-2A-Flpo
   

   Use

   Recombinase-mediated cassette exchange using flp …

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-2xFLAG-2xSTREP-CDK2

   Plasmid

   #172616

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged CDK2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDK2 (CDK2 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-eSpCas9-2A-GFP-sgRNA-HSD17B11

   Plasmid

   #161923

   Purpose

   To generate HSD17B11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against HSD17B11 exon 1.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   sgRNA targeting HSD17B11 exon 1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-FuG-B

   Plasmid

   #67523

   Purpose

   This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B envelope coating virus particle with HiRet by using this plasmid.

   Depositor

   Kazuto Kobayashi

   Article

   Kato et al Hum Gene Ther. 2011 Feb;22(2):197-206. d

   Insert

   Fusion Glycoprotein type B
   

   Use

   Lentiviral

   Tags

   Fusion protein of Vesicular stomatitis Indiana vi…

   Expression

   Mutation

   Promoter

   CAGGS

   Available Since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-nEF-Con/Foff 2.0-bREACHes-EYFP

   Plasmid

   #137146

   Purpose

   Intersectional viral expression of bREACHes-EYFP in cells expressing Cre AND NOT Flp

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Insert

   Con/Foff 2.0-bREACHes-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV2-U6gRNA5(GATA3(13))-PGKpuro2ABFP-W

   Plasmid

   #208548

   Purpose

   Lentiviral vector expressing gRNA targeting human GATA3

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   GATA3(13) (GATA3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV2-U6gRNA5(GATA3(12))-PGKpuro2ABFP-W

   Plasmid

   #208547

   Purpose

   Lentiviral vector expressing gRNA targeting human GATA3

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   GATA3(12) (GATA3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. MEK1C222

    Plasmid

    #164638

    Purpose

    Bacterial expression plasmid for His6-MEK1C222

    Depositor

    Ben Davis

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    G(-19)F/S222C/C277S/C376S

    Promoter

    T7

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-opCas12a-Ultra-6xNLS

    Plasmid

    #209024

    Purpose

    Lentiviral vector expressing human codon-optimized opCas12a-Ultra with 6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    opCas12a-Ultra-6xNLS-Flag-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and MYC

    Expression

    Mammalian

    Mutation

    E174R/M537R/S542R/F870L

    Promoter

    EF-1a

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP

    Plasmid

    #137150

    Purpose

    Intersectional viral expression of Arch3.3-EYFP in cells expressing Flp AND NOT Cre

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-Arch3.3-p2a-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    nEF

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRE-HRI-V5-IRES-BFP

    Plasmid

    #202435

    Purpose

    Lentiviral vector expression of HRI and TagBFP

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    HRI (EIF2AK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiCRISPRv2-sgCD20

    Plasmid

    #209749

    Purpose

    Lentiviral transfer plasmid to express Cas9 and a gRNA targeting the human CD20 gene, MS4A1.

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Insert

    MS4A1 (MS4A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCW57-Cx32-IRES-GCaMP6s

    Plasmid

    #216795

    Purpose

    Inducible bicistronic lentiviral plasmid for the simultaneous expression of Connexin 32 and cytosolic GCaMP6s

    Depositor

    Fabio Mammano

    Article

    Tettey-Matey et al Cell Commun Signal. 2024 Dec 5;22(1):589

    Insert

    Connexin 32-IRES-GCaMP6s (GJB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. HUHe variant click editor (CE1) - pCMV-T7-TGMV-nCas9-EcKlenow (JO659)

    Plasmid

    #208951

    Purpose

    A variant CE1 construct with TGMV HUH enconuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    TGMV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. HUHe variant click editor (CE1) - pCMV-T7-FBYNV-nCas9-EcKlenow (JO610)

    Plasmid

    #208950

    Purpose

    A variant CE1 construct with FBYNV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    FBYNV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. HUHe variant click editor (CE1) - pCMV-T7-RepBM-nCas9-EcKlenow (JO606)

    Plasmid

    #208949

    Purpose

    A variant CE1 construct with RepBM HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    RepBm-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. HUHe variant click editor (CE1) - pCMV-T7-TraI-nCas9-EcKlenow (JO604)

    Plasmid

    #208948

    Purpose

    A variant CE1 construct with TraI HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    TraI-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSH-Csy4-T2A-SpRFN

    Plasmid

    #85754

    Purpose

    Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).

    Depositor

    Lawrence Stanton

    Article

    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

    Insert

    Csy4-T2A-FokI-dCas9
    

    Use

    CRISPR

    Tags

    Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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