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pMSSM105: pCMV-HA-NLS-SpuFz1-T513K

Plasmid

#205276

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-T513K

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

T513K

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMSSM58: pCMV-HA-NLS-SpuFz1-D487K

Plasmid

#205274

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D487K

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

D487K

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMSSM59: pCMV-HA-NLS-SpuFz1-D300R

Plasmid

#205272

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D300R

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

D300R

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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EC.SurA.pTYB1.a21-281&390-428.wt

Plasmid

#12607

Depositor

David McKay

Article

Bitto et al J Biol Chem. 2003 Dec 5. 278(49):49316-2

Insert

Escherichia coli surA delta PPIase domain 2

Tags

intein

Expression

Bacterial

Mutation

residues 282-389 deleted

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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TOPO mKO2

Plasmid

#68442

Purpose

TOPO construct expressing mKusabira-Orange2 for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mKusabira-Orange2

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FRB-LplA(W37I)

Plasmid

#34840

Depositor

Alice Ting

Article

Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

Insert

FRB-LplA(W37I)

Tags

FLAG (internal fusion), HA, and His Tag

Expression

Mammalian

Mutation

LplA has Trp37 to Val mutation

Promoter

CMV

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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RAP2B

Plasmid

#55667

Purpose

expression of mouse RAP2B

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RAP2B (Rap2b Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pHPdestVenus

Plasmid

#24568

Depositor

Ingrid Lohmann

Article

Boy et al Genesis. 2010 Apr 22. [Epub ahead of pri

Insert

Use

Reporter construct

Tags

Venus

Available Since

May 19, 2010

Availability

Academic Institutions and Nonprofits only

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pPI184

Plasmid

#113221

Purpose

generic knockin vector for C-terminal tagging with mCherry

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Generic knockin vector for c-terminal tagging wit…

Tags

mCherry

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pmCIT-CFPGgVcl 1-419 control 2 FRET

Plasmid

#46283

Depositor

Susan Craig

Article

Chen et al J Biol Chem. 2006 Dec 29;281(52):40389-9

Insert

ECFP + Vcl 1-419 (VCL Chicken)

Tags

mCitrine

Expression

Mammalian

Mutation

aa 1-419

Promoter

CMV

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pJM410 ZF1x6-C mKate2 in TUPV1

Plasmid

#161526

Purpose

Inducible expression of mKate2 under the ZF1x6-C promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

mKate2

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF1x6-C

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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peSpCas9-J23109

Plasmid

#113152

Purpose

Plasmid constitutively expressing eSpCas9 protein

Depositor

Chong Zhang

Article

Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

Insert

eSpCas9

Expression

Bacterial

Mutation

K810A & K1003A & R1060A

Available Since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pPI464

Plasmid

#113216

Purpose

generic knockin vector for C-terminal tagging with mScarlet

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Generic knockin vector for c-terminal tagging wit…

Tags

mScarlet

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;basPEgfp

Plasmid

#90169

Purpose

contains a basal promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

basalPEgfp

Tags

EGFP

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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human K18

Plasmid

#14741

Depositor

M Bishr Omary

Article

Ku et al J Cell Biol. 1994 Oct . 127(1):161-71.

Insert

Keratin 18 (KRT18 Human)

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pET-3a-His-MBP-TEV-VC_Cas1_WT

Plasmid

#120364

Purpose

Expresses wildtype V-C Cas1

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

VC_Cas1

Use

CRISPR

Expression

Bacterial

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET-3a-His-MBP-TEV-VD_Cas1_WT

Plasmid

#120365

Purpose

Expresses wildtype V-D Cas1

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

VD_Cas1

Use

CRISPR

Expression

Bacterial

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pVC-CRISPR1

Plasmid

#120366

Purpose

Plasmid containing shortened version of upstream V-C CRISPR array for integration assays

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

CRISPR1

Use

CRISPR

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pVC-CRISPR

Plasmid

#120369

Purpose

Plasmid containing shortened versions of both natural V-C CRISPR arrays for preparing sequencing library

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Inserts

CRISPR1
CRISPR2

Use

CRISPR

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pVD_CRISPR

Plasmid

#120370

Purpose

Plasmid containing shortened version of V-D CRISPR array for integration assays

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

VD_CRISPR

Use

CRISPR

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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