We narrowed to 9,264 results for: pho;

1. pKLV2-U6gRNA5(CDK6-E2(12))-PGKpuro2ABFP-W

   Plasmid

   #200488

   Purpose

   Lentiviral vector expressing gRNA targeting human CDK6-E2

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   CDK6-E2(12) (CDK6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV2-U6gRNA5(CDK6-E1(1))-PGKpuro2ABFP-W

   Plasmid

   #200486

   Purpose

   Lentiviral vector expressing gRNA targeting human CDK6-E1

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   CDK6-E1(1) (CDK6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLV2-U6gRNA5(CDK6-E2(15))-PGKpuro2ABFP-W

   Plasmid

   #200489

   Purpose

   Lentiviral vector expressing gRNA targeting human CDK6-E2

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   CDK6-E2(15) (CDK6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV2-U6gRNA5(BRD9(91))-PGKpuro2ABFP-W

   Plasmid

   #200474

   Purpose

   Lentiviral vector expressing gRNA targeting human BRD9

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   BRD9(91) (BRD9 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6gRNA5(ARID2(14))-PGKpuro2ABFP-W

   Plasmid

   #200472

   Purpose

   Lentiviral vector expressing gRNA targeting human ARID2

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   ARID2(14) (ARID2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti gRNA KO OCLNx2 spCas9-T2A-iRFP670-P2A-puro

   Plasmid

   #208399

   Purpose

   codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, iRFP670 fluorescent protein and puromycin resistance

   Depositor

   Raphael Gaudin

   Article

   Baptiste Pradel et al bioRxiv

   Insert

   hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti gRNA KO OCLNx2 spCas9-T2A-Crimson-P2A-puro

   Plasmid

   #208398

   Purpose

   codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, E2-Crimson fluorescent protein and puromycin resistance

   Depositor

   Raphael Gaudin

   Article

   Baptiste Pradel et al bioRxiv

   Insert

   hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   E2-Crimson

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti gRNA KO OCLNx2 spCas9-T2A-mNeonGreen-P2A-puro

   Plasmid

   #208400

   Purpose

   codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, mNeonGreen fluorescent protein and puromycin resistance

   Depositor

   Raphael Gaudin

   Article

   Baptiste Pradel et al bioRxiv

   Insert

   hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUb FLAG-Mts

   Plasmid

   #195065

   Purpose

   Expresses FLAG-tagged Drosophila mts protein

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   microtubule star (mts Fly)
   

   Use

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   Promoter

   Ubi-p63e

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV10-3xFLAG-prs-hMIGA2(170-593)-25GS-6xhis

    Plasmid

    #192835

    Purpose

    Express codon-optimized human MIGA2 fragment 170-593 with a GS-linker and 6xhis tag at C-terminus (lipid transfer construct) in Expi293 cells

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    

    Use

    Tags

    3xFLAG-PreScission cleavage site and 6xhis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Coexpressed Free deadFPPS-NES

    Plasmid

    #182492

    Purpose

    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein deadFPPS-AcNES1 (GAL7 promoter). deadFPPS is ERG20(K197G-K254A), an inactive mutant of ERG20.

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Inserts

    FPPS
    deadFPPS-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10 and GAL7

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDR111_mannose_Stat1Klf6

    Plasmid

    #188400

    Purpose

    Expresses mouse transcription factors Stat-1 and Klf6 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Inserts

    Stat-1 (Stat1 Escherichia coli, Mouse)
    Klf6 (Klf6 Mouse)
    

    Use

    Tags

    PhoD secretion peptide

    Expression

    Bacterial

    Mutation

    Promoter

    D-mannose

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMY-CD90.2-P2A-BlastDYK

    Plasmid

    #163349

    Purpose

    Retroviral vector to express the surface marker CD90.2 and a DYK-tagged blasticidin resistance marker

    Depositor

    Teunis Geijtenbeek, Louis Ates

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    CD90.2 (Thy1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL3-Basic-ZC3H12D_PromoterMUT

    Plasmid

    #153067

    Purpose

    ZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporter

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    ZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Mutagenesis to disrupt 3 BHLHE4040 binding sites.…

    Promoter

    None

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZIP-hCMV-ZsGreen-P2A-Puro-IKZF1 sh1

    Plasmid

    #123324

    Purpose

    Lentiviral vector for expression of shRNA targeting IKZF1 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

    Insert

    shRNA targeting IKZF1 embedded in miR30 backbone (IKZF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZIP-hCMV-ZsGreen-P2A-Puro-IKZF1 sh2

    Plasmid

    #123325

    Purpose

    Lentiviral vector for expression of shRNA targeting IKZF1 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

    Insert

    shRNA targeting IKZF1 embedded in miR30 backbone (IKZF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5 FLAG membrane-bound lipin-1 (delta200)

    Plasmid

    #120278

    Purpose

    Expression of ER/Golgi-tethered lipin-1 in mammalian cells

    Depositor

    Sirio Dupont

    Article

    Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

    Insert

    Lipin-1 (Lpin1 Mouse)
    

    Use

    Tags

    FLAG and in-frame fusion with the delta200 Golgi-…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-hPC1_MTS_L4139P-eGFP-3HA

    Plasmid

    #108404

    Purpose

    Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Use

    Tags

    3xHA and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-mPC1_4119-eGFP

    Plasmid

    #108394

    Purpose

    Genomic region, including intron 45, to express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    variant ID rs13468401 missense variant R4237Q

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-mPC1_4148-3HA

    Plasmid

    #108398

    Purpose

    Express cytoplasmic tail mouse PC1 from amino acid 4148 to 4293

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    Pkd1 (Pkd1 Mouse)
    

    Use

    Tags

    3HA

    Expression

    Mammalian

    Mutation

    variant ID rs13468401 missense variant R4237Q

    Promoter

    CMV

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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