We narrowed to 2,123 results for: PORA

1. pET21-GFP-AURKA-mCherry

   Plasmid

   #99876

   Purpose

   Expression of GFP-AURKA-mCherry for protein purification in bacteria

   Depositor

   Marc Tramier

   Article

   Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   GFP and mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDisplay-GRAB_OTmut

   Plasmid

   #185385

   Purpose

   Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in mammalian cells

   Depositor

   Yulong Li

   Article

   Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   GPCR activation based oxytocin control sensor GRAB_OTmut
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-DRD2-V2tail-TevN-BLITz1-TetR-VP16

   Plasmid

   #89870

   Purpose

   expresses DRD2-iTango component in mammalian cells

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   DRD2-V2tail-TevN-BLITz1-TetR-VP16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   short isoform of DRD2 (deletion of 242-270)

   Promoter

   CMV

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBEST-p70a-UTR3-deGFP-6xHis-T500

   Plasmid

   #92224

   Purpose

   Expression of deGFP using the p70a promoter and UTR3

   Depositor

   Lital Alfonta

   Article

   Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

   Insert

   deGFP
   

   Use

   Synthetic Biology; Cell free protein synthesis (c…

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   p70a

   Available since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAS_4xG1 PylT sfGFP 150TAG

   Plasmid

   #154767

   Purpose

   plasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TAG

   Promoter

   EF1

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. XW17

   Plasmid

   #65835

   Purpose

   Punc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed for the convenience of cloning, most sites are compatible with pPD95.77)

   Depositor

   Kang Shen

   Article

   Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

   Insert

   OR74A quinic acid utilization activator (qa-1F)
   

   Use

   Tags

   mCherry

   Expression

   Worm

   Mutation

   The endogenous SphI, KpnI and SacI sites in QF ar…

   Promoter

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AID-BFP-loxP_myo2_neoR

   Plasmid

   #194054

   Purpose

   Dual-selection cassette plasmid for knocking-in AID-BFP into the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   IAA17 (AXR3 Mustard Weed)
   

   Use

   Tags

   bfp

   Expression

   Worm

   Mutation

   Promoter

   None

   Available since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLL7.0-iRFP670-micro

   Plasmid

   #135954

   Purpose

   Expresses an iLID micro (SspB R73Q)-iRFP670 fusion in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   iLID micro (SspB R73Q)
   

   Use

   Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAMV:nAChRgamma-eGFP

   Plasmid

   #29693

   Depositor

   Henry Lester

   Article

   Pantoja et al Biophys J. 2009 Jan . 96(1):226-37.

   Insert

   GFP tagged Mus musculus cholinergic receptor, nicotinic, gamma (Chrng Mouse)
   

   Use

   Tags

   AMV promotor and poly(A) tail

   Expression

   Bacterial

   Mutation

   eGFP inserted after position S380

   Promoter

   Available since

   July 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAS_4xhyb PylT sfGFP 150TAG

    Plasmid

    #154771

    Purpose

    plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRP_8xgRNA-FLuc

    Plasmid

    #135937

    Purpose

    Firefly luciferase reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minCMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28:LanII-57

    Plasmid

    #225074

    Purpose

    Expresses two precursor peptides and the lanthipeptide synthetase from LanII-57 (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA(A) (LanII-57)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRXIP-GFP-PKD C1ab

    Plasmid

    #221755

    Purpose

    Stable expression of GFP-PKD C1ab in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    Prkd1(138-343) (Prkd1 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    amino acids 138-343

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA^EcTyr

    Plasmid

    #218766

    Purpose

    encodes EcTyrRS-VSMA* and EcTyr-tRNA-TAG

    Depositor

    Abhishek Chatterjee

    Article

    Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

    Insert

    EcTyrRS-VSMA*
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y37V, D167G, D182S, F183M, L186A, D265R

    Promoter

    tacI

    Available since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr

    Plasmid

    #218767

    Purpose

    encodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAG

    Depositor

    Abhishek Chatterjee

    Article

    Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

    Insert

    EcTyrRS-pAAFRS-9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y37G, L71V, D182C, F183Y, L186C, D265R

    Promoter

    tacI

    Available since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lenti-Nova1-GFP

    Plasmid

    #217033

    Purpose

    Overexpression of Nova in DRG neurons promotes the "mature" splicing pattern observed in CNS neurons

    Depositor

    Chaolin Zhang

    Article

    Weyn-Vanhentenryck et al Nat Commun. 2018 Jun 6;9(1):2189. doi: 1

    Insert

    Nova1 (Nova1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TDP-43 mRuby K84ONBK

    Plasmid

    #216226

    Purpose

    TDP-43 with a C-terminal mRuby tag and Lysine 84 in the NLS mutated to a TAG stop codon for amber codon suppression.

    Depositor

    Jennifer Lee

    Article

    Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

    Insert

    Transactive response DNA binding protein of 43 kDa (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lysine 84 mutated to a TAG stop codon

    Promoter

    CMV Promoter

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EGFP-ST-P2A-pSC-CAAX

    Plasmid

    #207637

    Purpose

    A plasmid encoding EGFP fused to SpyTag (ST) and photocaged SpyCatcher (pSC) localized to the cell membrane via CAAX tag.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    EGFP-SpyTag-P2A-photocaged SpyCatcher-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Amber stop codon at SC's critical lysine

    Promoter

    CMV

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Aga2p-LOV-Turbo1-myc_pCTCON2

    Plasmid

    #199673

    Purpose

    expresses pre-evolved version of LOV-Turbo on the yeast surface

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo1
    

    Use

    Tags

    Aga2p and Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. iEscSnFR

    Plasmid

    #182807

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

    Insert

    iEscSnFR
    

    Use

    Tags

    6xHis, HA and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. iFluoxSnFR

    Plasmid

    #182808

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

    Insert

    iFluoxSnFR
    

    Use

    Tags

    6xHis, HA and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTV-Flnc-5prime-1000-24MS96T-3F_NeoR

    Plasmid

    #174881

    Purpose

    Targeting vector for generating Flnc STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag with homology arms for Flnc
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTV-Usp5-5prime-1000-24MS96T-3F_NeoR

    Plasmid

    #174885

    Purpose

    Targeting vector for generating Usp5 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag with homology arms for Usp5
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTV-Wnk1-5prime-1000-24MS96T-3F_NeoR

    Plasmid

    #174886

    Purpose

    Targeting vector for generating Wnk1 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag with homology arms for Wnk1
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLV-Hygro-EF1A-KR-Linker-eGFP

    Plasmid

    #176066

    Purpose

    EGFP fused to the C-terminus of a KR domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    KR
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET22b-ecDHFR-D27S/Y100F

    Plasmid

    #110827

    Purpose

    Bacterial expression of E.coli DHFR D27S/Y100F mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

    Insert

    ecDHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D27 mutated to S27, Y100 mutated to F100

    Promoter

    T7

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHW564 (Pmyo-2::cGAL-N(C1A)::let-858 3'UTR)

    Plasmid

    #107739

    Purpose

    mutated cGAL-N (C1A) driver under the control of C. elegans myo-2 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::cGAL(DBD)::gp41-1-N-intein(C1A)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    change the first cysteine of gp41-1 N-intein to a…

    Promoter

    C. elegans myo-2 promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHR-HNRNPA1C-mCh-Cry2WT

    Plasmid

    #101226

    Purpose

    hnRNPA1(186-320) fused to mCh-Cry2WT

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    hnRNPA1(186-320) (HNRNPA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Mfn2-YFP

    Plasmid

    #28010

    Depositor

    Richard Youle

    Article

    Karbowski et al J Cell Biol. 2002 Dec 23. 159(6):931-8.

    Insert

    mitofusin 2 (MFN2 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    silent S508S

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHR Grb2-TagBFP

    Plasmid

    #188631

    Purpose

    Human Grb2 C-terminally labeled with TagBFP

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    GRB2 (GRB2 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge