We narrowed to 10,936 results for: AGA

1. pKLV2-U6gRNA5(PDLIM1_Bru)-PGKpuroBFP-W

   Plasmid

   #211979

   Purpose

   Express gRNA against PDLIM1 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting PDLIM1 (PDLIM1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Human U6 promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFPC2-gephyrin S268E/270E

   Plasmid

   #199609

   Purpose

   Encodes N-terminal eGFP-tagged P1-gephyrin S268/270E for expression in mammalian cells.

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S268E/S270E

   Promoter

   CMV

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLCHKO_TK1_HPRT1_Lb_2

   Plasmid

   #155060

   Purpose

   Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and an intergenic site & HPRT1 using LbCas12a (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Empty Bridge Control Zfp462-Klf4SE

   Plasmid

   #127666

   Purpose

   Encodes for 4 gRNAs expressed from their individual hU6 promoters. Two gRNAs target the Zfp462 promoter while the other two target the Klf4 stretch enhancer.

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Insert

   gRNAs 129, 135, 115, 117
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAcGFP1-N1-Dsup-deltaC

   Plasmid

   #90023

   Purpose

   To express GFP-tagged Dsup lacking C-terminal region in mammalian cells transiently

   Depositor

   Takekazu Kunieda

   Article

   Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

   Insert

   Dsup
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Deletion of C-terminal region

   Promoter

   CMV

   Available Since

   Aug. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pStreptagII 3C EGFR EABR

   Plasmid

   #234994

   Purpose

   For production of Extracellular Vesicles (EVs), with Epithelial Growth Factor Receptor on their surface, Strep-tag II labeled on its N-terminus

   Depositor

   Daniel Leahy, Monica Gonzalez-Magaldi

   Article

   Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

   Insert

   EGFR-EABR (EGFR Human)
   

   Tags

   Streptag-II, HRV 3C site

   Expression

   Mammalian

   Available Since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHA StreptagII 3C NIS EABR

   Plasmid

   #234988

   Purpose

   For production of Extracellular Vesicles (EVs), with a Strep-tag II labeled Sodium-iodide symporter (NIS), and an HA epitope at its N-terminus on their surface

   Depositor

   Daniel Leahy, Monica Gonzalez-Magaldi

   Article

   Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

   Insert

   NIS-EABR (SLC5A5 Human)
   

   Tags

   HA, Streptag-II, HRV 3C site

   Expression

   Mammalian

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-ADAR2-2a-Fezf2 sesRNA-2a-tTA2-WPRE

   Plasmid

   #239027

   Purpose

   Expression of ADAR2, sesRNA in mammalian cells with hSyn promoter, with tTA2 as efRNA and ADAR2 overexpression.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for mouse Fezf2 (Fezf2 Mouse)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCXN2KS-Dsup

   Plasmid

   #90019

   Purpose

   To establish stable line expressing Dsup in constitutive manner

   Depositor

   Takekazu Kunieda

   Article

   Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

   Insert

   Dsup
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Circular 200,100 with interspersed loops (GAPDH)

    Plasmid

    #180183

    Purpose

    AAV vector carrying a guide RNA targeting the human GAPDH mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with interspersed loops (GAPDH Human)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Circular 200,100 with 8 bp bulge loops (GAPDH)

    Plasmid

    #170121

    Purpose

    AAV vector carrying a guide RNA targeting the human GAPDH mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with 8bp bulge loops (GAPDH Human)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLEX307_PDHA2S293A

    Plasmid

    #115197

    Purpose

    Lentiviral transduction and expression of PDHA2S293A into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    PDHA2S293A (PDHA2 Human)
    

    Use

    Lentiviral

    Mutation

    p.S293A

    Promoter

    EF1alpha

    Available Since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPHAGE-EF1α-Luc-C20orf24-3’UTR-WT

    Plasmid

    #128551

    Purpose

    Lentiviral constitutive expression of Firefly luciferase under control of WT 3'UTR of human C20orf24.

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. LADL Bridge + Target Zfp462-Klf4SE

    Plasmid

    #127668

    Purpose

    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a and hU6

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. CDK11B gRNA (BRDN0001146011)

    Plasmid

    #77065

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK11B

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK11B (CDK11B Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV2-U6gRNA5(ETS2_5-5)-PGKpuroBFP-W

    Plasmid

    #211959

    Purpose

    Express gRNA against ETS2 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting ETS2 (ETS2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Human U6 promoter

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKLV2-U6gRNA5(FOXH1_5-4)-PGKpuroBFP-W

    Plasmid

    #211963

    Purpose

    Express gRNA against FOXH1 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting FOXH1 (FOXH1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Human U6 promoter

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKLV2-U6gRNA5(ETS2_5-2)-PGKpuroBFP-W

    Plasmid

    #211958

    Purpose

    Express gRNA against ETS2 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting ETS2 (ETS2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Human U6 promoter

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR223-PARK7H126A

    Plasmid

    #115180

    Purpose

    Gateway cloning of PARK7H126A into any destination vector

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    PARK7H126A (PARK7 Human)
    

    Use

    Gateway cloning into appropriate destination vect…

    Mutation

    p.H126A

    Available Since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR223-PARK7E163K

    Plasmid

    #115181

    Purpose

    Gateway cloning of PARK7E163K into any destination vector

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    PARK7E163K (PARK7 Human)
    

    Use

    Gateway cloning into appropriate destination vect…

    Mutation

    p.E163K

    Available Since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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