Addgene

We narrowed to 3,177 results for: Ubc

Showing: 741 - 760 of 3177 results

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Ubiquitin K48C

Plasmid

#86588

Purpose

Bacterial expression of Ubiquitin K48C for cross linking studies

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

Ubiquitin (UBC Human)

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

TEV site; K48C

Promoter

Tac

Available since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pET23a-HsRPS27a

Plasmid

#69561

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

RPS27A (RPS27A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only
FUW-RLuc-T2A-PuroR

Plasmid

#102320

Purpose

expression of r-luciferase

Depositor

Rudolf Jaenisch

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

Renilla luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Ubc

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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Ubiquitin K63C

Plasmid

#86589

Purpose

Bacterial expression of Ubiquitin K63C for cross linking studies

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

Ubiquitin (UBC Human)

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

TEV site; K63C

Promoter

Tac

Available since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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Ubiquitin G76C

Plasmid

#86583

Purpose

Bacterial expression of Ubiquitin G76C for cross linking studies

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

Ubiquitin (UBC Human)

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

TEV site; G76C

Promoter

Tac

Available since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pEntry Tshld(FKBP F36V)-GFP

Plasmid

#53260

Purpose

pEntry Tshld(FKBP F36V)-GFP is a product of BP recombination reaction between attB flanked Tshld(F36V) system-encoded PCR product and pDonor 201 plasmid.

Depositor

Matthew Pratt

Article

Lin et al Chembiochem. 2014 Mar 11. doi: 10.1002/c

Insert

Tshld(FKBP F36V)-GFP

Use

Tags

EGFP, Flag, and HA

Expression

Mutation

FKBP F36V

Promoter

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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Ubiquitin K6C

Plasmid

#86587

Purpose

Bacterial expression of Ubiquitin K6C for cross linking studies

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

Ubiquitin (UBC Human)

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

TEV site; K6C

Promoter

Tac

Available since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pUB_SMFLuc0---MS2

Plasmid

#210568

Purpose

Expresses SM(FLAG)-nonoptimal Firefly with 3'UTR MS2 hairpins

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-nonoptimal FLuc

Use

Lentiviral and Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Mutation

Promoter

UBC human

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_SMFLuc100---MS2

Plasmid

#210570

Purpose

Expresses SM(FLAG)-optimal Firefly with 3'UTR MS2 hairpins

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal FLuc

Use

Lentiviral and Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Mutation

Promoter

UBC human

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_OptλHAramp-FLuc100

Plasmid

#210577

Purpose

Expresses Optimal lambda HA-Optimal Firefly luciferase

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Optimal lambda HA-Optimal Firefly luciferase

Use

Tags

lambda HA

Expression

Mammalian

Mutation

Promoter

UBC human

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_NonoptλHAramp-FLuc100

Plasmid

#210579

Purpose

Expresses Nonoptimal lambda HA-Optimal Firefly luciferase

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Nonoptimal lambda HA-Optimal Firefly luciferase

Use

Lentiviral and Luciferase

Tags

lambda HA

Expression

Mammalian

Mutation

Promoter

UBC human

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Ubiquitin K6C/G76C

Plasmid

#86584

Purpose

Bacterial expression of Ubiquitin K6C/G76C

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

Ubiquitin (UBC Human)

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

TEV site; K6C/G76C

Promoter

Tac

Available since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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Ubiquitin K48C/G76C

Plasmid

#86585

Purpose

Bacterial expression of Ubiquitin K48C/G76C

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

Ubiquitin (UBC Human)

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

TEV site; K48C/G76C

Promoter

Tac

Available since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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Ubiquitin K63C/G76C

Plasmid

#86586

Purpose

Bacterial expression of Ubiquitin K63C/G76C

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

Ubiquitin (UBC Human)

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

TEV site; K63C/G76C

Promoter

Tac

Available since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pSLHDR03_mCherry_tCD19_unique guide

Plasmid

#124062

Purpose

Construction of 1st donor for scarless genome editing with unique guide

Depositor

Matthew Porteus

Article

Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

Inserts

mCherry
tCD19

Use

Mouse Targeting; Human targeting

Tags

Expression

Mutation

Promoter

Human UbC modified

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pLJC5-LAMP1-RFP-3xHA

Plasmid

#102932

Purpose

Express a lysosomal Tag

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

LAMP1

Use

Lentiviral

Tags

RFP-HA

Expression

Mutation

Promoter

ubc

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pTBL3478 PB-PEmax-mTagBFP2

Plasmid

#226664

Purpose

PiggyBac cargo vector encoding PEmax and mTagBFP2

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Inserts

PEmax
mTagBFP2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG and UBC

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pLJC5-LAMP1-RFP-2xFlag

Plasmid

#102931

Purpose

Express a lysosomal Tag

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

LAMP1

Use

Lentiviral

Tags

mRFP-2xFlag

Expression

Mutation

Promoter

ubc

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pFudio-Cpg15-IRES2-TdTomatoW

Plasmid

#134309

Purpose

Expresses cpg15 and TdTomato upon coexpression with Cre recombinase

Depositor

Elly Nedivi

Article

Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

Inserts

cpg15 (Nrn1 Mouse, Rat)
TdTomato

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

UbC

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pEYFP-C3-HA-Ubiquitin

Plasmid

#28236

Depositor

Michael Mancini

Article

Stenoien et al Nat Cell Biol. 2002 Oct . 4(10):806-10.

Insert

Ubiquitin (UBB Human)

Use

Tags

EYFP and HA

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2011

Availability

Academic Institutions and Nonprofits only

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