We narrowed to 3,078 results for: camp

1. pcDNA3-EGFP-AC8(1-106)

   Plasmid

   #181958

   Purpose

   Residues 1-106 of rat adenylyl cyclase 8 (AC8) tagged with EGFP.

   Depositor

   Jin Zhang

   Article

   Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

   Insert

   EGFP-AC8(1-106) (Adcy8 Rat)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. CRAF_S259A_pcDNA3.1

   Plasmid

   #86505

   Purpose

   Expresses CRAF_S259A in mammalian cells

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

   Insert

   CRAF (RAF1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S259A

   Promoter

   CMV

   Available Since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEM-HE-2xLyn-ERex-Venus-bPAC(F198Y)

   Plasmid

   #165489

   Purpose

   Xenopus oocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

   Depositor

   Thomas Oertner

   Article

   Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

   Insert

   2xLyn-ERex-Venus-bPAC(F198Y)
   

   Use

   Cdna expression, xenopus oocyte

   Tags

   Venus

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV gfaABC1D 2xLyn-ERex-Venus-bPAC(F198Y)

   Plasmid

   #171117

   Purpose

   Astrocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

   Depositor

   Thomas Oertner

   Article

   Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

   Insert

   2xLyn-ERex-Venus-bPAC(F198Y)
   

   Use

   AAV

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   gfaABC1D

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CaMKII-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

   Plasmid

   #165493

   Purpose

   Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

   Depositor

   Thomas Oertner

   Article

   Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

   Insert

   2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
   

   Use

   AAV

   Tags

   darkVenus

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV hSyn1 CaRhAC T2A tDimer

   Plasmid

   #101722

   Purpose

   - humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neurons

   Depositor

   Thomas Oertner

   Article

   Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

   Inserts

   CatRhAC
   red fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mutation

   E497K,C569D

   Promoter

   human Synapsin1 promotor and ribosomal skip seque…

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. CIAR_pcDNA5/FRT/TO

   Plasmid

   #86502

   Purpose

   Expresses CIAR in mammalian cells. Used to generate Flp-In stables.

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

   Insert

   CIAR (SOS1 Human)
   

   Use

   Frt

   Tags

   Expression

   Mammalian

   Mutation

   T968L in SOScat

   Promoter

   CMV (tet operator)

   Available Since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Syn-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

   Plasmid

   #165492

   Purpose

   Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

   Depositor

   Thomas Oertner

   Article

   Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

   Insert

   2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
   

   Use

   AAV

   Tags

   darkVenus

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-RanV19 E1RA2

   Plasmid

   #118317

   Purpose

   Expression of human Ran

   Depositor

   Philip Stork

   Article

   Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

   Inserts

   RanV19 (RAN Human)
   EPac1RA2 (RAPGEF3, RAPGEF4 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   in-frame insertion of RanV19 into GFPEpac1RA2 and…

   Promoter

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. NC1 pGLUE WTX (1-804)

    Plasmid

    #36956

    Depositor

    Randall Moon

    Article

    Major et al Science. 2007 May 18;316(5827):1043-6.

    Insert

    WTX (AMER1 Human)
    

    Use

    Tags

    CBP tag, HA, and streptag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

11. xCIAR_pcDNA5/FRT/TO

    Plasmid

    #86504

    Purpose

    Expresses inactive control CIAR (xCIAR) in mammalian cells. Used to generate Flp-In stables.

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    xCIAR (SOS1 Human)
    

    Use

    Frt

    Tags

    Expression

    Mammalian

    Mutation

    F929A and T968L in SOScat

    Promoter

    CMV (tet operator)

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSV-PKImut-v2

    Plasmid

    #45067

    Purpose

    Expression of intactive PKI (PKA inhibitor) mutant

    Depositor

    Richard Maurer

    Insert

    cAMP-dependent protein kinase inhibitor alpha
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Arg 20 & 21 to Glycines

    Promoter

    RSV

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-DIO-EF1α-mKate2-PDE4D3-Cat

    Plasmid

    #169128

    Purpose

    AAV plasmid containing a mKate2 and a truncated PDE4D3

    Depositor

    Georg Nagel

    Article

    Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

    Has Service

    AAV1

    Insert

    mKate2-PDE4DCatL (PDE4D Human)
    

    Use

    AAV and Cre/Lox

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    truncated, Catalatic domain only

    Promoter

    EF1a

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA 3.1(-) mouse C/EBP delta

    Plasmid

    #12559

    Depositor

    Peter Johnson

    Article

    McCauslin et al J Biol Chem. 2006 Jun 30;281(26):17681-8

    Insert

    CCAAT/Enhancer-binding Protein delta (Cebpd Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S2G introduced during cloning

    Promoter

    Available Since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX6P1-3xMBT_D355N

    Plasmid

    #46988

    Purpose

    Inducible expression of 3xMBT (D355N) from L3MBTL1 as a GST fusion protein in E.coli

    Depositor

    Or Gozani

    Article

    Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:

    Insert

    Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)
    

    Use

    Tags

    GST and PreScission cleavage site

    Expression

    Bacterial

    Mutation

    Mutation aspartic acid 355 to asparagine (D355N);…

    Promoter

    tac

    Available Since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-RIαΔ(D/D+linker)-EGFP

    Plasmid

    #181843

    Purpose

    GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-(D/D+linker)EGFP (PRKAR1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of residues 12-112 in PKA RIα.

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)-RIα(D/D+linker)-EGFP

    Plasmid

    #181842

    Purpose

    EGFP-tagged docking/dimerization (D/D) and linker region of PKA RIα; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-(D/D+linker)EGFP (PRKAR1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Contains only residues 12-112 of PKA RIα.

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. CRAF_S29A_pcDNA3.1

    Plasmid

    #86506

    Purpose

    Expresses CRAF_S29A in mammalian cells [Edit]

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    CRAF (RAF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S29A

    Promoter

    CMV

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)-PKAcat(G1A)-mCherry

    Plasmid

    #181852

    Purpose

    mCherry-tagged PKA catalytic subunit lacking N-terminal myristoylation site; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    PKAcat-mCherry (PRKACA Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Glycine residue immediately after start site in P…

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)-DnaJB1-PKAcat-mCherry

    Plasmid

    #181850

    Purpose

    mCherry-tagged DnaJB1-PKA catalytic subunit fusion; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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