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Plasmid#222078PurposeAeBlue visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.DepositorTypeEmpty backboneUseChromoprotein-marked pvs1 backboneTagsExpressionMutationPromoterAvailable sinceJan. 27, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits
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pCRISPRainbow-DONOR1
Plasmid#75398PurposeCRISPRainbow-DONOR1DepositorInsertCm(R)-CcdB
UseCRISPRTagsnoExpressionMutationPromoterBacteriaAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAct-GW-HygroR
Plasmid#149610PurposeGateway compatible expression plasmid for Drosophila and establishing stable cell lines by Hygromycin selection. Actin5c ubiquitous promoter.DepositorInsertGateway cassette (ccdb + Chlor.R.)
UseTagsExpressionInsectMutationPromoterAct5cAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSWAP_Entry_H2_beta_CM
Plasmid#184870PurposepSwap plasmid part with H2_beta homology arms and chloramphenicol cassetteDepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 2, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSWAP_Entry_H1_alpha
Plasmid#184865PurposepSwap plasmid part with H1_alpha homology armsDepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSWAP_Entry_HAIB
Plasmid#184869PurposeFor scarless integration into the chromosome, HAIB plasmid can be usedin place of the HA, INS and HB modules.DepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSWAP_Entry_H2_beta_GM
Plasmid#184871PurposepSwap plasmid part with H2_beta homology arms and gentamycin cassetteDepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSWAP_Entry_INS
Plasmid#184867PurposepSwap plasmid part with insert fragment moduleDepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSWAP_Entry_HA
Plasmid#184866PurposepSwap plasmid part with HA homology armsDepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSWAP_Entry_HB
Plasmid#184868PurposepSwap plasmid part with HB homology armsDepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNJB80
Plasmid#51518PurposeGateway entry vector with attL5 and attL2 sites flanking a CmR and ccdB gene. In our hands, the multisite reaction still proceeds efficiently.DepositorTypeEmpty backboneUseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFunnyfarm
Plasmid#24755DepositorInsertCAT-ccdB cassette
UseGateway-adapted entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_FL Gcn4
Plasmid#232955PurposeGalactose iduced expression of full-length Gcn4 in yeastDepositorInsertGcn4
UseTagsTEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_DBDHA
Plasmid#232987PurposeGalactose iduced expression of Gcn4 DBDAHA in yeastDepositorInsertDBD
UseTags3xHA and TEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 L123A
Plasmid#232969PurposeGalactose iduced expression of Gcn4 L123A in yeastDepositorInsertGcn4 L123A
UseTagsTEV cleavage siteExpressionYeastMutationL123APromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Swap2
Plasmid#232965PurposeGalactose iduced expression of Gcn4 k0.19 in yeastDepositorInsertGcn4 k0.19
UseTagsTEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Swap3
Plasmid#232960PurposeGalactose iduced expression of Gcn4 k0.47 in yeastDepositorInsertGcn4 k0.47
UseTagsTEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 W120A
Plasmid#232962PurposeGalactose iduced expression of Gcn4 W120A in yeastDepositorInsertGcn4 W120A
UseTagsTEV cleavage siteExpressionYeastMutationW120APromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_DBD
Plasmid#232985PurposeGalactose iduced expression of Gcn4 DBD in yeastDepositorInsertGcn4 DBD
UseTagsTEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 F124I
Plasmid#232964PurposeGalactose iduced expression of Gcn4 F124I in yeastDepositorInsertGcn4 F124I
UseTagsTEV cleavage siteExpressionYeastMutationF124IPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only